Acta Neuropathologica

, Volume 85, Issue 3, pp 248–255 | Cite as

Small heat-shock protein is expressed in meningiomas and in granulofilamentous inclusion bodies

  • N. Yokoyama
  • T. Iwaki
  • J. E. Goldman
  • J. Tateishi
  • M. Fukui
Regular Papers
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Summary

The cellular expression of estrogen receptor-related small heat-shock protein (HSP27) in meningiomas was investigated immunologically. A cytoplasmic distribution of HSP27 was demonstrated in surgical specimens of 22 of 26 cases with meningiomas and cultured meningioma cells derived from two individuals. By Western blotting, HSP27 was detected in every tissue homogenate of 17 cases studied. Thus, HSP27 appears to be constitutively expressed in most meningiomas. In anaplastic portions of one papillary meningioma, there were numerous granulofilamentous inclusion bodies [Goldman JE et al. (1980) Cancer 46:156–161]. The inclusion bodies were immunopositive for HSP27 despite the negativity of the tumor cytoplasms. Thus, HSP27 seems to participate in the formation of certain inclusion bodies in meningioma cells, like αB-crystallin which participates in the formation of Rosenthal fibers in astrocytes.

Key words

Meningioma Heat-shock protein Small heat-shock protein Ubiquitin Inclusion body 
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References

  1. 1.
    Adams DJ, McGuire WL (1985) Quantitative enzyme-linked immunosorbent assay for the estrogen-regulated M r 24,000 protein in human breast tumors: correlation with estrogen and progesteron receptors. Cancer Res 45:2445–2449Google Scholar
  2. 2.
    Adams DJ, Hajj H, Edwards DP, Bjercke RJ, McGuire WL (1983) Detection of an M r24,000 estrogen-regulated protein in human breast cancer by monoclonal antibody. Cancer Res 43:4297–4301Google Scholar
  3. 3.
    Adams EF, Schrell UMH, Fahlbusch R, Thierauf P (1990) Hormonal dependency in cerebral meningiomas. 2. In vitro effect of steroids, bromocriptine, and epidermal growth factor on growth of meningiomas. J Neurosurg 73:750–755Google Scholar
  4. 4.
    Arrigo A-P, Suhan JP, Welch WJ (1988) Dynamic change in the structure and intracellular locale of the mammalian lowmolecular weight heat shock protein. Mol Cell Biol 8:5059–5071Google Scholar
  5. 5.
    Bickerstaff ER, Small JM, Guest IA (1958) The relapsing course of certain meningiomas in relation to pregnancy and menstruation. J Neurol Neurosurg Psychiatry 21:89–91Google Scholar
  6. 6.
    Bloemendal H (1977) The vertebrate eye lens: a useful system for the study of fundamental biological process on a molecular level. Science 197:127–138Google Scholar
  7. 7.
    Burdon RH (1986) Heat shock and the heat shock proteins. Biochem J 240:313–324Google Scholar
  8. 8.
    Cahill DW, Bashirelahi N, Solomon LW, Dalton T, Salcman M, Ducker TB (1984) Estrogen and progesteron receptors in meningiomas. J Neurosurg 60:985–993Google Scholar
  9. 9.
    Ciocca DR, Adams DJ, Edwards DP, Bjercke RJ, McGuire WL (1983) Distribution of an estrogen-regulated protein with a molecular weight of 24,000 in normal and malignant human tissues and cells. Cancer Res 43:1204–1210Google Scholar
  10. 10.
    Ciocca DR, Puy LA, Fasoli LC (1989) Study of estrogen receptor, progesteron receptor, and the estrogen-regulated M sr 24,000 protein in patient with carcinomas of the endometrium and cervix. Cancer Res 49:4298–4303Google Scholar
  11. 11.
    Donnel MS, Meyer GA, Donegan WL (1979) Estrogen receptor protein in meningiomas. J Neurosurg 50:499–502Google Scholar
  12. 12.
    Edwards DP, Adams DJ, Savage N, McGuire WL (1981) Specific protein synthesis by estrogen in human breast cancer. J Steroid Biochem 15:247–259Google Scholar
  13. 13.
    Fuqua SAW, Blum-Salingaros M, McGuire WL (1989) Induction of the estrogen-regulated “24K” protein by heat shock. Cancer Res 49:4126–4129Google Scholar
  14. 14.
    Goldman JE, Horoupian DS, Johnson AB (1980) Granulofilamentous inclusions in a meningioma. Cancer 46:156–161Google Scholar
  15. 15.
    Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139Google Scholar
  16. 16.
    Hickey E, Brandon SE, Potter R, Stein G, Stein J, Weber LA (1986) Sequence and organization of genes encoding the human 27 kDa heat shock protein. Nucleic Acids Res 14:4127–4145Google Scholar
  17. 17.
    Ingolia TD, Craig EA (1982) Four small Drosophila heat shock proteins are related to each other and to mammalian α-crystallin. Proc Natl Acad Sci USA 79:2360–2364Google Scholar
  18. 18.
    Iwaki T, Kume-Iwaki A, Liem RKH, Goldman JE (1989) αB-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain. Cell 57:71–78Google Scholar
  19. 19.
    Iwaki T, Iwaki A, Miyazono M, Goldman JE (1991) Preferential expression of αB-crystallin in astrocytic elements of neuroectodermal tumors. Cancer 68:2230–2240Google Scholar
  20. 20.
    Kasantikul V, Brown WJ (1980) Estrogen receptors in acoustic neurilemmomas. Surg Neurol 15:105–109Google Scholar
  21. 21.
    Kato M, Herz F, Kato S, Hirano A (1992) Expression of stress-response (heat-shock) protein 27 in human brain tumors: an immunohistochemical study. Acta Neuropathol 83:420–422Google Scholar
  22. 22.
    Kepes JJ (1982) Meningiomas: biology, pathology, and differential diagnosis. Masson Publishing USA, New York, pp 12–19Google Scholar
  23. 23.
    Klemenz R, Fröhli E, Steiger RH, Schäfer R, Aoyama A (1991) αB-crystallin is a small heat shock protein. Proc Natl Acad Sci USA 88:3682–3656Google Scholar
  24. 24.
    Landry J, Bernier D, Chrétien P, Nicole LM, Tanguay RM, Marceau N (1982) Synthesis and degradation of heat shock proteins during development and decay of thermotolerance. Cancer Res 42:2457–2461Google Scholar
  25. 25.
    Markwell MAK, Haas SM, Bieber LL, Tolbert NE (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87:206–210Google Scholar
  26. 26.
    Martuza RL, MacLaughlin DT, Ojemann RG (1981) Specific estradiol binding in schwannomas, meningiomas, and neurofibromas. Neurosurgery 9:665–671Google Scholar
  27. 27.
    Michelson JJ, New PFJ (1969) Brain tumour and pregnancy. J Neurol Neurosurg Psychiatry 32:305–307Google Scholar
  28. 28.
    Oesterreich S, Schunck H, Benndorf R, Bielka H (1991) Cisplatin induces the small heat shock protein HSP25 in Ehrlich ascites tumor cells. Biochem Biophys Res Commun 82:7815–7819Google Scholar
  29. 29.
    Pauli D, Tonka CH, Tissieres A, Arrigo AP (1990) Tissuespecific expression of heat shock protein HSP27 during Drosophila melanogaster development. J Cell Biol 111:817–828Google Scholar
  30. 30.
    Schlesinger M (1986) Heat shock proteins: the search for functions. J Cell Biol 103:321–325Google Scholar
  31. 31.
    Schrell UMH, Adams EF, Fahlbusch R, Greb R, Jirikowski G, Prior R, Ramalho-Ortigao FJ (1990) Hormonal dependency of cerebral meningiomas. 1. Female sex steroid receptors and their significance as specific markers for adjuvant medical therapy. J Neurosurg 73:743–749Google Scholar
  32. 32.
    Shin RW, Iwaki T, Kitamoto T, Tateishi J (1991) Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues. Lab Invest 64:693–702Google Scholar
  33. 33.
    Shoenberg BS, Christine BW, Whisnant JP (1975) Nervous system neoplasms and primary malignancies of other sites. The unique association between meningiomas and breast cancer. Neurology 25:705–712Google Scholar
  34. 34.
    Thomas SR, Lengyel JA (1986) Ecdysteroid-regulated heat shock gene expression during Drosophila melanogaster development. Dev Biol 115:434–438Google Scholar
  35. 35.
    Tilzer LL, Plapp FV, Evans JP, Stone ED, Alward K (1982) Steroid receptor proteins in human meningiomas. Cancer 49:633–636Google Scholar
  36. 36.
    Tomokane N, Iwaki T, Tateishi J, Iwaki A, Goldman E (1991) Rosenthal fibers share epitopes with αB-crytallin, glial fibrillary acidic protein, and ubiquitin, but not with vimentin. Immunoelectron microscopy with colloidal gold. Am J Pathol 138:875–885Google Scholar
  37. 37.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedGoogle Scholar
  38. 38.
    Zülch KJ (1986) Brain tumors: their biology and pathology, 3rd edn. Springer-Verlag, Berlin Heidelberg, pp 357–383Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • N. Yokoyama
    • 1
  • T. Iwaki
    • 2
  • J. E. Goldman
    • 3
  • J. Tateishi
    • 2
  • M. Fukui
    • 1
  1. 1.Department of Neurosurgery, Neurological Institute, Faculty of MedicineKyushu University 60FukuokaJapan
  2. 2.Department of Neuropathology, Neurological Institute, Faculty of MedicineKyushu University 60FukuokaJapan
  3. 3.Department of PathologyColumbia University College of Physicians and SurgeonsNew YorkUSA

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