Sexual Plant Reproduction

, Volume 7, Issue 5, pp 311–312 | Cite as

The isolation of viable egg cells of wheat (Triticum aestivum L.)

  • Miklós Kovács
  • Beáta Barnabás
  • Erhard Kranz
Short Communication
Received:
Accepted:

Abstract

The isolation of viable egg cells of wheat has been achieved without enzymatic maceration of the ovules. 2,4-D applied to the stigmas resulted 3 to 7 days later in soft ovule tissues which disintegrated upon mechanical manipulation. The isolated egg cells were viable even 2 h after isolation. Their morphology corresponded to that of the in situ egg cells. The mean isolation frequency was 20% (two egg cells per ten ovules).

Key words

Egg cell apparatus in situ Egg cell isolation Wheat 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Miklós Kovács
    • 1
  • Beáta Barnabás
    • 1
  • Erhard Kranz
    • 2
  1. 1.Cell Biology DepartmentAgricultural Research Institute of the Hungarian Academy of SciencesMartonvásárHungary
  2. 2.University of Hamburg, Centre for Applied Plant Molecular Biology, AMP II, Institute of General BotanyHamburgGermany

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