Molecular and Cellular Biochemistry

, Volume 112, Issue 2, pp 155–162 | Cite as

Regulation of calcium transport in pancreatic acinar plasma membranes from guinea pig

  • Rajesh Mahey
  • Bruce G. Allen
  • Michael A. Bridges
  • Sidney Katz
Article
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Accepted:

Abstract

The regulation of the guinea-pig pancreatic acinar plasma membrane Ca2+ pump by protein kinase A, protein kinase C and calmodulin was investigated. The results were compared with the effects of these regulators on the high affinity Ca2+-ATPase found in this membrane preparation. The catalytic subunit of cyclic AMP-dependent protein kinase stimulated Ca2+ transport 2-fold, but had no effect on Ca2+-dependent ATPase activity. Purified protein kinase C, the phorbol ester 12-O-tetradecanoyl phorbol-13-acetate and diacylglycerol derivative, 1-stearoyl-2-arachidonoyl-sn-glycerol, failed to stimulate the Ca2+-uptake but augmented the Ca2+-dependent ATPase activity. Exogenously added calmodulin failed to stimulate either activity. In addition, two antagonists of calmodulin activity, trifluoperazine and compound 48/80 produced a concentration-dependent inhibition of Ca2+-transport. These data suggest the presence of endogenous calmodulin within guinea-pig pancreatic acinar plasma membranes. Both calmodulin antagonists failed to influence the Ca2+-dependent ATPase activity. The ability of boiled extracts from guinea-pig pancreatic acinar plasma membranes to stimulate the Ca2+-ATPase activity in calmodulin-depleted erythrocyte plasma membranes confirmed the presence of endogenous calmodulin. Our results imply a role for calmodulin and cAMP-dependent protein kinase, but not protein kinase C, in the regulation of Ca2+ efflux from pancreatic acinar cells. These results also provide further evidence suggesting that the high affinity Ca2+-ATPase does not catalyze the plasma membrane Ca2+-transport activity observed in pancreatic acini.

Key words

Ca2+-pump Ca2+-ATPase protein kinase A protein kinase C calmodulin 

Abbreviations

AMP

adenosine 5′-monophosphate

CaM

calmodulin

EDTA

ethylenediaminetetraacetate

EGTA

ethylene glycol bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

PKA

protein kinase A

PKC

protein kinase C

PS

phosphatidylserine

SA-DG

1 stearoyl-2-arachidonoyl-sn-glycerol

TFP

trifluoperazine

TPA

12-O-tetradecanoyl phorbol-13-acetate

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Rajesh Mahey
    • 1
  • Bruce G. Allen
    • 1
  • Michael A. Bridges
    • 2
  • Sidney Katz
    • 1
  1. 1.Division of Pharmacology and Toxicology, Faculty of Pharmaceutical SciencesUniversity of British ColumbiaVancouverCanada
  2. 2.Department of Pharmacology, Faculty of MedicineUniversity of British ColumbiaVancouverCanada

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