Molecular and Cellular Biochemistry

, Volume 32, Issue 3, pp 153–160 | Cite as

Insulin action in intact mouse diaphragm I.

Activation of Glycogen Synthase through Stimulation of Sugar Transport and Phosphorylation
  • Yoram Oron
  • Joseph Larner
Article
Received:

Summary

The incubation of intact mouse diaphragms with insulin caused a dose and time dependent increase in the independent activity of glycogen synthase in tissue extracts. 2-deoxyglucose (2–10 mm) alone markedly stimulated the conversion of glycogen synthase to the independent activity under conditions in which tissue ATP concentrations were not affected. The incubation of diaphragms with both insulin and 2-deoxyglucose resulted in a greater than additive effect. Insulin stimulated the uptake of 2-deoxyglucose into mouse diaphragms, accumulating as 2-deoxyglucose-6-phosphate. The accumulation of 2-deoxyglucose-6-phosphate correlated well with the increase in the independent activity of glycogen synthase and with the activation of glycogen synthase phosphatase in tissue extracts. The uptake of 3-0-methyl glucose was also markedly stimulated by insulin, without affecting the activity of glycogen synthase. Both glucose-6-phosphate and 2-deoxyglucose-6-phosphate stimulated the activation of endogenous glycogen synthase phosphatase activity in muscle homogenates. We conclude that insulin, in addition to its effects in the absence of exogenous sugars, increases the independent activity of glycogen synthase through increased sugar transport resulting in increased concentrations of sugar-phosphates which promote the activity of glycogen synthase phosphatase.

Keywords

Phosphatase Activity Insulin Action Tissue Extract Independent Activity Sugar Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

GS

Glycogen synthase

GS-I

Glycogen synthase activity independent of G6P

GS-D

Glycogen synthase activity dependent on G6P

G6P

Glucose-6-phosphate

ATP

Adenosine triphosphate

EDTA

Ethylene diamine tetracetic acid

Mops

Morpholinopropane sulfonic acid

2DG

2-Deoxy glucose

3-0-MG

3-0-Methyl glucose

tricine

N-tris(Hydroxymethyl)methyl glycine Enzymes: Glycogen Synthase — UDPGlucose — Glycogen Glucosyl — Transferase (EC 2.4.1.11)

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Copyright information

© Dr. W. Junk bv. Publishers 1980

Authors and Affiliations

  • Yoram Oron
    • 1
  • Joseph Larner
    • 1
  1. 1.Department of PharmacologyUniversity of Virginia School of MedicineCharlottesvilleUSA

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