Advertisement

Cell and Tissue Research

, Volume 185, Issue 1, pp 63–68 | Cite as

Alpha-1-antitrypsin immunoreactivity in islet cells of adult human pancreas

  • M. B. Ray
  • V. J. Desmet
  • W. Gepts
Article

Summary

Alpha-1-antitrypsin immunoreactivity was demonstrated by immunofluorescence technique in the peripheral islet cells of all ten normal adult human pancreata examined; normal adult human liver was negative. The specificity of the reactions was confirmed by applying various control tests including absorption of the specific antisera with purified alpha-1-antitryspin, inhibition and blocking tests and by ensuring the monospecificity of the antisera used. The findings suggest that the pancreatic islet may be an additional source of alpha-1-antitryspin.

Key words

Pancreas (Human) Islet cells Alpha-1-antitrypsin Immunofluorescence 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allan, B.J., Zager, N.I., Keller, P.J.: Human pancreatic proteins: Amylase, proelastase and trypsinogen. Arch. Biochem. Biophys. 136, 529–540 (1970)Google Scholar
  2. Benitez-Bribiesca, L., Freyre, R., De La Vega, G.: Alpha-1-antitrypsin in human mast cells: Immunofluorescent localization. Life Sci. 13, 631–638 (1973)Google Scholar
  3. Bhan, A.K., Grand, R.J., Colten, H.R., Alper, C.A.: Liver in alpha-1-antitrypsin deficiency. Morphological observations and in vitro synthesis of alpha-1-antitrypsin. Pediat. Res. 10, 35–40 (1976)Google Scholar
  4. Deconinck, J.F., Potvliege, P.R., Gepts, W.: The ultrastructure of human pancreatic islets. 1. The islets of adults. Diabetologia 7, 266–282 (1971)Google Scholar
  5. Gordon, H., Dixon, J., Roger, J.C.: Alpha-1-antitrypsin accumulation in the livers of emphysematous patients with alpha-1-antitrypsin deficiency. Human Path. 3, 361–370 (1972)Google Scholar
  6. Lieberman, J.: The multiple causes of alpha-1-antitrypsin deficiency. Pathet Biol. 23, 517–520 (1975)Google Scholar
  7. Lieberman, J., Mittman, C., Gordon, H.W.: Alpha-1-antitrypsin in the livers of patients with emphysema. Science 1975, 63–65 (1972)Google Scholar
  8. Pearse, A.G.E.: Histochemistry, theoretical and applied, Vol. 1, p. 665, 3rd edn. Boston: Little, Brown & Company 1968Google Scholar
  9. Ray, M.B., Desmet, V.: Immunofluorescent detection of alpha-1-antitrypsin in paraffin embedded liver tissue. J. clin. Path. 28, 717–721 (1975)Google Scholar
  10. Rowley, P.T., Miller, L.L.: Serum antitrypsin synthesis by the isolated perfused rat liver. Proc. Soc. Exptl. Bio. Med., 148, 145–150 (1975)Google Scholar
  11. Sharp, H.L.: Alpha-1-antitrypsin deficiency. Hospital Practice 6, 83–96 (1971)Google Scholar
  12. Valverde, I., Villanueva, M.L., Lozano, I., Marco, J.: Presence of glucagon immunoreactivity in the globulin fraction of human plasma (“Big Plasma Glucagon”). J. clin. Endocr. 39, 1090–1098 (1974)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • M. B. Ray
    • 1
  • V. J. Desmet
    • 1
  • W. Gepts
    • 2
  1. 1.Departement Medische Navorsing, Academisch Ziekenhuis Sint RafaëlLaboratorium voor Histochemie en CytochemieLeuvenBelgium
  2. 2.Laboratorium voor Anatomopathologie Universitair Ziekenhuis BrugmanBrusselBelgium

Personalised recommendations