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Theoretical and Applied Genetics

, Volume 83, Issue 4, pp 515–527 | Cite as

Physical maps of Nicotiana chloroplast DNA constructed by an efficient procedure

  • Ding-I Yang
  • Teng-Yung Feng
  • Chi-Chang Chen
  • Yiu-Kay Lai
Originals

Summary

The restriction profiles of chloroplast DNA (cpDNA) from Nicotiana tabacum, N. sylvestris, N. plumbaginifolia, and N. otophora were obtained with respect to AvaI, BamHI, BglI, HindIII, PstI, PvuII, SalI, and XhoI. An efficient mapping method for the construction of cpDNA physical maps in Nicotiana was established via a computer-aided analysis of the complete cpDNA sequence of N. tabacum for probe selection. The efficiency of this approach is demonstrated by the determination of cpDNA maps from N. sylvestris, N. plumbaginifolia, and N. otophora with respect to all of the above restriction endonucleases. The size and basic structure of the cpDNA from the three species are almost identical, with an addition of approximately 80 bp in N. plumbaginifolia. The restriction patterns and hence the physical maps between N. tabacum and N. sylvestris cpDNA are identical and there is no difference in the Pvull digests of cpDNA from all four species. Restriction site variations in cpDNA from different species probably result from point mutations, which create or eliminate a particular cutting site, and they were observed spanning the whole chloroplast molecule but highly concentrated in both ends of the large, single-copy region. The results presented here will be used for the forthcoming characterization of chloroplast genomes in the interspecies somatic hybrids of Nicotiana, and will be of great value in completing the exploration of the phylogenetic relationships within this already extensively studied genus.

Key words

Nicotiana Chloroplast DNA Restriction patterns Physical mapping Restriction site variations 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Ding-I Yang
    • 1
  • Teng-Yung Feng
    • 2
  • Chi-Chang Chen
    • 3
  • Yiu-Kay Lai
    • 1
  1. 1.Institute of Life ScienceNational Tsing Hua UniversityHsinchuTaiwan 30043, Republic of China
  2. 2.Institute of Botany, Academic SinicaTaipeiTaiwan 11529, Republic of China
  3. 3.Department of BotanyNational Taiwan UniversityTaipeiTaiwan 10764, Republic of China

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