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Theoretical and Applied Genetics

, Volume 83, Issue 2, pp 194–200 | Cite as

Segregation of random amplified DNA markers in F1 progeny of conifers

  • J. E. Carlson
  • L. K. Tulsieram
  • J. C. Glaubitz
  • V. W. K. Luk
  • C. Kauffeldt
  • R. Rutledge
Originals

Summary

The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products (“RAPD” markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.

Key words

Conifers genetic markers Linkage mapping Random amplified DNA markers 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. E. Carlson
    • 1
  • L. K. Tulsieram
    • 1
  • J. C. Glaubitz
    • 1
  • V. W. K. Luk
    • 1
  • C. Kauffeldt
    • 2
  • R. Rutledge
    • 2
  1. 1.The Biotechnology Laboratory and the Faculty of Forestry at the University of British ColumbiaVancouver, B.C.Canada
  2. 2.Forestry Canada, Petawawa National Forestry InstituteChalk RiverCanada

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