Theoretical and Applied Genetics

, Volume 92, Issue 6, pp 673–679 | Cite as

A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers

  • M. E. Devey
  • J. C. Bell
  • D. N. Smith
  • D. B. Neale
  • G. F. Moran
Article

Abstract

A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pedigree. A total of 208 loci were analyzed including 165 restriction fragment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 1382 cM. Thirteen loci were unlinked to any other marker. Of the RFLP loci that were mapped, 93 were detected by loblolly pine (P. taeda L.) cDNA probes that had been previously mapped or evaluated in that species. The remaining 72 RFLP loci were detected by radiata pine probes from a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 1∶1 ratio were mapped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to genetic studies in P. radiata and other pine species.

Key words

Pinus radiata Genetic linkage map RFLP RAPD 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. E. Devey
    • 1
  • J. C. Bell
    • 1
  • D. N. Smith
    • 1
  • D. B. Neale
    • 2
  • G. F. Moran
    • 1
  1. 1.CSIRO Forestry and Forest ProductsAustralian Capital TerritoryAustralia
  2. 2.Institute of Forest Genetics, USDA Forest Service, Pacific Southwest Research StationBerkeleyUSA

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