Molecular and Cellular Biochemistry

, Volume 158, Issue 2, pp 189–192 | Cite as

Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

  • Xuan Z. Ding
  • Robert C. Smallridge
  • Richard J. Galloway
  • Juliann G. Kiang
Short Communication
Received:
Accepted:

Abstract

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.

Key words

reverse transcriptase polymerase chain reaction gene method HSF1 HSF2 heat shock proteins epithelia 
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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Xuan Z. Ding
    • 1
  • Robert C. Smallridge
    • 1
  • Richard J. Galloway
    • 1
  • Juliann G. Kiang
    • 1
  1. 1.Department of Clinical Physiology, Division of MedicineWalter Reed Army Institute of ResearchWashingtonUSA

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