Theoretical and Applied Genetics

, Volume 93, Issue 1–2, pp 183–189

Somatic hybrid plants between the forage legumes Medicago sativa L. and Medicago arborea L.

  • E. Nenz
  • F. Pupilli
  • F. Damiani
  • S. Arcioni
Article

Abstract

Interspecific somatic hybrid plants were obtained by symmetrical electrofusion of mesophyll protoplasts of Medicago sativa with callus protoplasts of Medicago arborea. Somatic hybrid calli were picked manually from semi-solid culture medium after they were identified by their dual color in fluorescent light. Twelve putative hybrid calli were selected and one of them regenerated plants. The morphogenesis of the somatic hybrid calli was induced by the synthetic growth regulator 1,2 benzisoxazole-3-acetic acid. Somatic hybrid plants showed intensive genome rearrangements, as evidenced by isozyme and RFLP analysis. The morphology of somatic hybrid plants was in general intermediate between the parents. The production of hybrids by protoplast fusion between sexually incompatible Medicago species is related to the in vitro respon siveness of the parental protoplasts. The possibility of using somatic hybrid plants in alfalfa breeding is discussed.

Key words

M. sativa M. arborea Protoplast electrofusion Somatic hybrids Genome rearrangements 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • E. Nenz
    • 1
  • F. Pupilli
    • 1
  • F. Damiani
    • 1
  • S. Arcioni
    • 1
  1. 1.Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del C.N.R.PerugiaItaly

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