Theoretical and Applied Genetics

, Volume 93, Issue 1–2, pp 57–64 | Cite as

A genetic map of melon (Cucumis melo L.) with RFLP, RAPD, isozyme, disease resistance and morphological markers

  • S. Baudracco-Arnas
  • M. Pitrat
Article

Abstract

One hundred and ten markers were analysed for linkage in 218 F2 plants derived from two divergent cultivars (‘Védrantais’ and ‘Songwhan Charmi’) of Cucumis melo (L.). Thirty-four RFLPs, 64 RAPDs, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cM of the melon genome. RAPD and RFLP markers detected similar polymorphism levels. RFLPs were largely due to base substitutions rather than insertion/deletions. Twelve percent of markers showed distorted segregation. Phenotypic markers consisted of two resistance genes against Fusarium wilt (Fom-1 and Fom-2), one gene (nsv) controlling the resistance to melon necrotic spot virus, one gene (Vat) conferring resistance to Aphis gossypii, and a recessive gene for carpel numbers (3 vs 5 carpels: p).

Key words

Cucumis melo RFLP RAPD Molecular markers Genetic map 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adam-Blondon AF, Sevignac M, Dron M (1994) A genetic map of common bean to localize specific resistance genes against anthracnose. Genome 37:915–924Google Scholar
  2. Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278Google Scholar
  3. Arumanagathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218Google Scholar
  4. Balagué C, Watson CF, Turner AJ, Rouge P, Picton S, Pech JC, Grierson D (1993) Isolation of a ripening and wound-induced cDNA from Cucumber melo L. encoding a protein with homology to the ethylene-forming enzyme. Eur J Bioche, 212:27–34Google Scholar
  5. Baudracco-Arnas S (1995) A simple and inexpensive method for DNA extraction from Cucumis melo L. Cucurbit Genet Coop Rep 18:50–51Google Scholar
  6. Coudriet DL, Kishaba AN, Bohn GW (1981) Inheritance of resistance to muskmelon necrotic spot virus in a melon aphid-resistant breeding line of muskmelon. J Am Soc Hort Sci 106:789–791Google Scholar
  7. Dane F (1983) Cucurbits. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part B. pp 369–390Google Scholar
  8. Ellis THN, Turner L, Hellens RP, Lee D, Harker CL, Enard C, Domoney C, Davies DR (1992) Linkage maps in pea. Genetics 130:649–663PubMedGoogle Scholar
  9. Esquinas JT (1981) Allozyme variation and relationships among Spanish land-races of Cucumis melo L. Kulturpflanze 29: 337–352Google Scholar
  10. Giese H, Holm-Jensen AG, Mathiassen H, Kjœr B, Rasmussen SK, Bay H, Jensen J (1994) Distribution of RAPD markers on a linkage map of barley. Hereditas 120:267–273Google Scholar
  11. Heun M, Helentjaris T (1993) Inheritance of RAPDs in F1 hybrids of corn. Theor Appl Genet 85:961–968Google Scholar
  12. Kennard WC, Poetter K, Dijkhuizen A, Meglic V, Staub JE, Havey MJ (1994) Linkage among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet 89:42–48Google Scholar
  13. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  14. Lander ES, Botstein ED (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199PubMedGoogle Scholar
  15. Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337Google Scholar
  16. Lark KG, Weisemann JM, Matthews BF, Palmer R, Chase K, Macalma T (1993) A genetic map of soyben (Glycine max L.) using an intraspecific cross of two cultivars: ‘Minosy’ and ‘Noir1’ Theor Appl Genet 86:901–906Google Scholar
  17. Lefebvre V, Palloix A, Rives M (1993) Nuclear RFLP between pepper cultivars (Capsicum annum L.) Euphytica 71:189–199Google Scholar
  18. Marrou J (1967) Amélioration des méthodes de transmission mécanique des virus par adsorption des inhibiteurs d'infection sur le charbon végétal. CR Acad Agric France 53:972–981Google Scholar
  19. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829Google Scholar
  20. Neuhausen SL (1992) Evaluation of restriction fragment length polymorphism in Cucumis melo. Theor Appl Genet 83:379–384Google Scholar
  21. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993Google Scholar
  22. Perl-Treves R, Zamir D, Navot N, Galun E (1985) Phytogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny. Theor Appl Genet 71: 430–436Google Scholar
  23. Pitrat M (1991) Linkage groups in Cucumis melo L. J Hered 82:406–411Google Scholar
  24. Pitrat M (1994) Gene list for Cucumis melo L. Cucurbit Genet Coop Rep 17:135–148Google Scholar
  25. Pitrat M, Lecoq H (1980) Inheritance of resistance to cucumber mosaic virus transmission by Aphis gossypii in Cucumis melo. Phytopathology 70:958–961Google Scholar
  26. Quiot JB, Douine L, Gebre Selassie K (1979) Fréquence des principales viroses identifiées dans une exploitation maraîchère du Sud-Est de la France. Ann Phytopathol 11:283–290Google Scholar
  27. Ramachandran C, Seshadri VS (1986) Cytological analysis of the genome of cucumber (Cucumis sativus L) and muskmelon (Cucumis melo L.). Z Pflanzenzuchtg 96:25–38Google Scholar
  28. Risser G (1973) Etude de I'hérédité de la resistance du melon (Cucumis melo) aux races 1 et 2 de Fusarium oxysporum f. sp. melonis. Ann Amélior Plant 23:259–263Google Scholar
  29. Risser G, Mas P (1965) Mise en évidence de plusieurs races de Fusarium oxysporum f. sp. melonis. Ann Amélior Plant 15:405–408Google Scholar
  30. Rosa JT (1928) The inheritance of flower types in Cucumis and Citrullus. Hilgardia 3:233–250Google Scholar
  31. Shattuck-Eidens DM, Bell RN, Neuhausen SL, Helentjaris T (1990) DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing. Genetics 126:207–217Google Scholar
  32. Staub JE, Fredrick L, Marty TL (1987) Electrophoretic variation in cross-compatible wild diploid species of Cucumis. Can J Bot 65:792–798Google Scholar
  33. Wendel JF, Weeden NF (1990) Visualization and interpretation of plant isozymes. In: Soltis, DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides Press, Portland. Oregon, pp 5–45Google Scholar
  34. Williams JGK, Kubelik AR, Livak KJ, Rafalsky JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • S. Baudracco-Arnas
    • 1
  • M. Pitrat
    • 1
  1. 1.INRA, Station d'Amélioration des Plantes MaraîchèresMontfavet CedexFrance

Personalised recommendations