Theoretical and Applied Genetics

, Volume 93, Issue 1–2, pp 48–56 | Cite as

RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley

  • M. Schönfeld
  • A. Ragni
  • G. Fischbeck
  • A. Jahoor


Three new major, race-specific, resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) were identified in three barley lines, ‘RS42-6*O’, ‘RS137-28*E’, and ‘HSY-78*A’, derived from crosses with wild barley (Hordeum vulgare ssp. spontaneum). The resistance gene origining from wild barley in line ‘RS42-6*O’, showed a recessive mode of inheritance, whereas the other wild barley genes were (semi)-dominant. RFLP mapping of these three genes was performed in segregating F2 populations. The recessive gene in line ‘RS42-6*O’, was localized on barley chromosome 1S (7HS), while the (semi)-dominant genes in lines ‘RS137-28*E’, and ‘HSY-78*A’, were localized on chromosomes 1L (7HL) and 7L (5HL), respectively. Closely linked RFLP clones mapped at distances between 2.6cM and 5.3 cM. Hitherto, specific loci for powdery mildew resistance in barley had not been located on these chromosomes. Furthermore, tests for linkage to the unlocalized resistance gene Mlp revealed free segregation. Therefore, these genes represent new loci and new designations are suggested: mlt (‘RS42-6*O’), Mlf (‘RS137-28*E’), and Mlj (‘HSY-78*A’). Comparisons with mapped QTLs for mildew resistance were made and are discussed in the context of homoeology among the genomes of barley (H-vulgare), wheat (Triticum aestivum), and rye (Secale cereale). Duplications of RFLP bands detected in the neighbourhood of Mlf and mlt might indicate an evolutionary interrelationship to the Mla locus for mildew resistance.

Key words

Hordeum vulgare ssp. spontaneum Erysiphe graminis f. sp. hordei Mildewresistance RFLP mapping Homoeology 


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  1. Anderson JA, Ogihara Y, Sorells ME, Tanksley SD (1992) Development of a chromosomal-arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043Google Scholar
  2. Aslam M, Schwarzbach E (1980) An inoculation technique for quantitative studies of brown rust resistance in barley. Phytopathol Z 99:87–91Google Scholar
  3. Backes G, Foroughi-Wehr B, Graner A, Fischbeck G, Wenzel G, Jahoor A (1995) Localization of quantitative loci (QTLs) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor Appl Genet 90:294–302Google Scholar
  4. Beckmann JS, Søller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67:35–43Google Scholar
  5. Chao S, Sharp PJ, Worland AJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group-7 chromosomes. Theor Appl Genet 78:495–504Google Scholar
  6. Devos KM, Atkinson MD, Chinoy CN, Liu CJ, Gale MD (1992) RFLP-based genetic map of the homoeologous group-3 chromosomes of wheat and rye. Theor Appl Genet 83:931–939Google Scholar
  7. Devos KM, Millan T, Gale MD (1993) Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85:784–792Google Scholar
  8. Driscoll CJ, Bielig LM (1968) Mapping of the Transec wheat-rye translocation. Can J Genet Cytol 10:421–425Google Scholar
  9. Ellingboe AH (1976) Genetics of host parastic interactions. In: Heitefuss R, Williams PH (eds) Encyclopedia of Plant Physiology New Series, vol. 4. Physiological plant pathology, pp 761–778 Springer, Berlin New YorkGoogle Scholar
  10. Ellis JG, Lawrence GJ, Finnegan EJ, Anderson PA (1995) Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci USA 92:4185–4188Google Scholar
  11. Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  12. Fischbeck G, Schwarzbach E, Sobel Z, Wahl I (1976) Types of protection against barley powdery mildew in Germany and Israel selected from Hordeum spontaneum In: Gaul H (eds) Barley genetics III. Proc 3rd Int Barley Genet Symp. Garching, pp 412–417Google Scholar
  13. Flor HH (1955) Host-parasite interaction in flax rust — its genetics and other implication. Phytopathology 45:680–685Google Scholar
  14. Giese H, Holm-Jensen AG, Jensen HP, Jensen J (1993) Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet 85:897–900Google Scholar
  15. Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374Google Scholar
  16. Görg R, Hollricher K, Schultz-Lefert P (1993) Functional analysis and RFLP-mediated mapping of the Mlg resistance locus in barley. Plant Jour 3:857–866Google Scholar
  17. Graner A, Bauer E (1993) RFLP mapping of the ym4 vims resistance gene in barley. Theor Appl Genet 86:689–693Google Scholar
  18. Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256Google Scholar
  19. Graner A, Bauer E, Kellermann A, Kirchner S, Muraya JK, Jahoor A, Wenzel G (1993) Progress of RFLP-map construction in winter barley. Barley Genet Newslett 23:53–59Google Scholar
  20. Hart GE, Gale MD, McIntosh RA (1993) Linkage maps of Triticum aestivum (hexaploid wheat, 2n = 42, Genomes A, B) and D and itT. tauschii 2n = 14, Genome D. In: O'Brien SJ (ed) Genetic maps, Cold Spring Harbor Laboratory Press, Book 6 Plants, 6.204–6.219Google Scholar
  21. Hartl L, Weiss H, Zeller FJ, Jahoor A (1993) Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum). Theor Appl Genet 86:959–963Google Scholar
  22. Hartl L, Weiss H, Stephan U, Zeller FJ, Jahoor A (1995) Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor Appl Genet 90:601–606Google Scholar
  23. Heun M (1992) Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35:1019–1025Google Scholar
  24. Hilbers S, Fischbeck G, Jahoor A (1992) Localization of the Laevigatum resistance gene MILA against powdery mildew in the barley genome by the use of RFLP markers. Plant Breed 109:334–338Google Scholar
  25. Hinze K, Thomson RD, Ritter E, Salmini F, Schluze-Lefert P (1991) Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley. (Hordeum vulgare). Proc Natl Acad Sci USA 88:3691–3695Google Scholar
  26. Hulbert SH, Bennetzen JL (1991) Recombination at the Rp1 locus of maize. Mol Gen Genet 226:377–382Google Scholar
  27. Jahoor A (1987) Mehltauresistenz israelischer Wildgersten — Resistenzspektrum, Vererbung, Lokalisierung (dissertation), Technical University of Munich/WeihenstephanGoogle Scholar
  28. Jahoor A, Fischbeck G (1987a) Genetical studies of resistance of powdery mildew in barley lines derived from Hordeum spontaneum collected from Israel. Plant Breed 99:265–273Google Scholar
  29. Jahoor A, Fischbeck G (1987b) Sources of resistance to powdery mildew in barley lines derived from Hordeum spontaneum collected in Israel. Plant Breed 99:274–281Google Scholar
  30. Jahoor A, Ludwig A, Fischbeck G (1989) New genes for powdery mildew resistance in Hordeum spontaneum-derived lines allelic or closely linked to the Mlp locus. Barley Genet Newslett 19:23–26Google Scholar
  31. Jahoor A Backes G, Graner A, Herrmann RG, Fischbeck G (1991) Development of RFLP markers for barley. Plant Breed 107:73–76Google Scholar
  32. Jia JZ, Miller TE, Reader SM, Gale MD (1994) RFLP tagging of gene Pm12 for powdery mildew resistance in wheat (Triticum aestivum). Science in China, Series B, Chemistry, Life Science and Earth Sciences 37:531–537Google Scholar
  33. Jørgensen, JH (1993) Coordinator's report: disease and pest resistance genes. Barley Genet Newslett 22:110–134Google Scholar
  34. Kilian A, Kudrna DA, Kleinhofs A, Yano M, Kurata N, Steffenson B, Sasaki T (1995) Rice-barley synteny and its application to saturation mapping of the barley Rpg1 region. Nucleic Acids Res 23:2729–2733Google Scholar
  35. Kintzios S, Jahoor A, Fischbeck G (1995) Powdery-mildew-resistance genes Mla29 and Mla32 in H. spontaneum-derived winter barley lines. Plant Breed 114:265–266Google Scholar
  36. Kølster P, Munk L, Stølen P, Løhde J (1986) Near-isogenic barley lines with genes for resistance to powdery mildew. Crop Sci 26:903–907Google Scholar
  37. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  38. Lander E, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  39. Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophtera infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137:67–77Google Scholar
  40. Lincoln SE, Lander ES (1992) Systematic detection of errors in genetic linkage data. Genomics. 14:604–610Google Scholar
  41. Lutz J, Hsam SLK, Limpert E, Zeller FJ (1995) Chromosomal location of powdery mildew resistance genes in common wheat (Triticum aestivum L.). 2. Genes Pm2 and Pm19 from Aegilops squarrosa L. Heredity 74:152–156Google Scholar
  42. Ma ZQ, Sorrels ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3 and Pm4 in wheat. Genome 37:871–875Google Scholar
  43. McIntosch RA, Baker EP (1968) Cytogenetical studies in wheat. IV. Chromosome location and linkage studies involving the Pm2 locus for powdery mildew resistance. Euphytica 19: 71–77Google Scholar
  44. Miller TE, Reader SM (1987) A guide to the homoeology of chromosomes within the Triticeae. Theor Appl Genet 74:214–217Google Scholar
  45. Moseman JG (1955) Sources of resistance to powdery mildew of barley. Plant Dis Rep 39:967–972Google Scholar
  46. Moseman JG (1959) Host-pathogen interaction of the genes for resistance in Hordeum vulgare and for pathogenicity in Erysiphe graminis f. ssp. hordei. Phytopatholgy 49:469–472Google Scholar
  47. Namuth DM, Lapitan NLV, Gill KS, Gill BS (1994) Comparative RFLP mapping of Hordeum vulgare and Triticum tauschii. Theor Appl Genet 89:865–872Google Scholar
  48. Paterson AH, Wing RA (1993) Genomic mapping in plants. Curr Opin Biotechnol 4:142–147Google Scholar
  49. Ronald PC, Albano B, Tabien R, Abenes L, Wu KS, McCouch S, Tanksley SD (1992) Genetic and physical analysis of the rice bacterial blight disease locus, Xa21. Mol Gen Genet 236: 113–120Google Scholar
  50. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedGoogle Scholar
  51. Saghai-Maroof MA, Zhang Q, Biyashev RM (1994) Molecular marker analyses of powdery mildew in barley. Theor Appl Genet 88:733–740Google Scholar
  52. Schüller C, Backes G, Fischbeck G, Jahoor A (1992) RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley. Theor Appl Genet 84:330–338Google Scholar
  53. Sharp PJ, Kreis M, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290Google Scholar
  54. Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of whaet RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348Google Scholar
  55. Sherman JD, Fenwick AL, Namuth DM, Lapitan NLV (1995) A barley RFLP map: alignment of three barley maps and comparisons to Gramineae species. Theor Appl Genet 91:681–690Google Scholar
  56. Suiter KA, Wendel JF, Case JS (1983) LINKAGE: a PASCAL computer program for the detection and analysis of genetic linkage. J Hered 74:203–204Google Scholar
  57. Tanksley S (1983) Molecular markers in plant breeding. Plant Mol Biol Rep 1:3–8Google Scholar
  58. Torp J, Jensen HP, Jørgensen JH (1978) Powdery mildew resistance genes in 106 northwest European spring barley variaties. Kgl Vet-og Landbohysk Arsskr 1978:75–102Google Scholar
  59. Tosa Y, Sakai K (1990) The genetics of resistance of hexaploid wheat to wheatgrass powdery mildew fungus. Genome 33: 225–230Google Scholar
  60. Wang ZY, Second G, Tanksley SD (1992) Polymorphism and phylogenetic relationships among species in the genus Oryza as determined by analysis of nuclear RFLPs. Theor Appl Genet 83:565–581Google Scholar
  61. Wiberg A (1974) Genetical studies of spontaneous sources of ressitance to powdery mildew in barley. Hereditas 77:89–148Google Scholar
  62. Zeller FJ, Lutz J, Remlein EI, Limpert E, Koenig J (1993) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). II. French cultivars. Agronomie 13:201–207Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Schönfeld
    • 1
  • A. Ragni
    • 2
  • G. Fischbeck
    • 1
  • A. Jahoor
    • 1
  1. 1.Lehrstuhl für Pflanzenbau und-Züchtung der TU MünchenWeihen-stephanGermany
  2. 2.Bio Integrated Technology S.r.1PantallaItaly

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