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Cell and Tissue Research

, Volume 242, Issue 1, pp 33–39 | Cite as

Ultrastructural organization of the glomerular basement membrane as revealed by a deep-etch replica method

  • Hitoshi Kubosawa
  • Yoichiro Kondo
Article

Summary

The fine structure of the glomerular basement membrane was re-evaluated by using a deep-etch replica method.

The structure of the laminae rarae interna and externa of the rat glomerular basement membrane was basically identical in that 6 to 8 nm fibrils were interconnected to form a three-dimensional, polygonal network. The size of the mesh was quite variable but most often ranged from 20 to 25 nm in width. In addition, a zipper-like substructure of the epithelial slit diaphragm was observed. By contrast, the lamina densa was composed of closely packed particles.

After exposure of the bovine glomerular basement membrane to ultrasonic waves or trypsin, the particles of the lamina densa were effectively removed. The underlying structure showed the fibrillar network closely resembled that seen in the laminae rarae of the rat glomerular basement membrane.

The glomerular basement membrane thus revealed was as principally composed of a fibrillar network, which might be regularly arranged units of type-IV collagen. Numerous fine particles, most likely proper components of the glomerular basement membrane, were attached onto this basic fibrillar structure, giving rise to a morphologic appearance different from that of the laminae rarae.

Key words

Deep-etching Glomerular basement membrane Epithelial slit diaphragm Ultrasonic waves Trypsin Rat 

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References

  1. Carlin B, Jaffe R, Bender B, Chung AE (1981) Entactin, a novel basal lamina-associated sulfated glycoprotein. J Biol Chem 256:5209–5214Google Scholar
  2. Carlson EC, Meezan E, Brendel K, Kenney MC (1981) Ultrastructural analyses of control and enzyme-treated isolated renal basement membranes. Anat Rec 200:421–436Google Scholar
  3. Caulfield JP (1979) Alterations in the distribution of Alcian Bluestaining fibrillar anionic sites in the glomerular basement membrane in aminonucleoside nephrosis. Lab Invest 40:503–511Google Scholar
  4. Caulfield JP, Farquhar MG (1978) Loss of anionic sites from the glomerular basement membrane in aminonucleoside nephrosis. Lab Invest 39:505–512Google Scholar
  5. Farquhar MG, Wissig SL, Palade GE (1961) Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall. J Exp Med 113:47–66Google Scholar
  6. Heuser JE, Salpeter SR (1979) Organization of acetylcholine receptors in quick frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane. J Cell Biol 82:150–173Google Scholar
  7. Inoué S, Leblond CP, Laurie GW (1983) Ultrastructure of Reichert's membrane, a multilayered basement membrane in the parietal wall of the rat yolk sac. J Cell Biol 97:1524–1537Google Scholar
  8. Kanwar YS, Farquhar MG (1979) Anionic sites in the glomerular basement membrane — In vivo and in vitro localization to the laminae rarae by cationic probes. J Cell Biol 81:137–153Google Scholar
  9. Kefalides MA, Alper R, Clark CC (1979) Biochemistry and metabolism of basement membranes. Int Rev Cytol 61:167–228Google Scholar
  10. Latta H (1970) The glomerular capillary wall. J Ultrastruct Res 32:526–544Google Scholar
  11. Laurie GW, Leblond CP, Inoué S, Martin GR, Chung A (1984) Fine structure of the glomerular basement membrane and immunolocalization of five basement membrane components to the lamina densa (basal lamina) and its extensions in both glomeruli and tubules of the rat kidney. Am J Anat 169:463–481Google Scholar
  12. Makino H (1982) Molecular sieve in rat glomerular basement membrane as revealed by negative staining. Acta Med Okayama 36:371–382Google Scholar
  13. Makino H (1983) Changes in the molecular sieve of glomerular basement membrane in rats with Masugi nephritis. Renal Physiol 6:266–274Google Scholar
  14. Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:656–677Google Scholar
  15. Misra RP (1972) Isolation of glomeruli from mammalian kidneys by graded sieving. Am J Clin Pathol 58:135–139Google Scholar
  16. Mynderse LA, Hassell JR, Kleinman HK, Martin GR, Martinez-Hernandez A (1983) Loss of heparan sulfate proteoglycan from glomerular basement membrane of nephrotic rats. Lab Invest 48:292–302Google Scholar
  17. Ota Z, Makino H, Miyoshi A, Hiramatsu M, Takahashi K, Ofuji T (1979) Molecular sieve in glomerular basement membrane as revealed by electron microscopy. J Electron Microsc 28:20–28Google Scholar
  18. Pappenheimer JR (1953) Passage of molecules through capillary walls. Physiol Rev 33:387–423Google Scholar
  19. Pappenheimer JR (1955) Über die Permeabilität der Glomerulummembranen in der Niere. Klin Wochenschr 33:362–365Google Scholar
  20. Rhodin J (1955) Electron microscopy of the glomerular capillary wall. Exp Cell Res 8:572–574Google Scholar
  21. Rodewald R, Karnovsky MJ (1974) Porous substructure of the glomerular slit diaphragm in the rat and mouse. J Cell Biol 60:423–433CrossRefPubMedGoogle Scholar
  22. Sawada H (1982) The fine structure of the bovine Descemet's membrane with special reference to biochemical nature. Cell Tissue Res 226:241–255Google Scholar
  23. Timpl R, Martin RG (1982) Components of basement membranes. In: Furthmayr H (ed) Immunochemistry of the extracellular matrix vol. 2, Applications. CRC Press, Boca Raton, FL, pp 119–150Google Scholar
  24. Timpl R, Wiedemann H, Delden VV, Furthmayr H, Kühn K (1981) A network model for the organization of type IV collagen molecules in basement membranes. Eur J Biochem 120:203–211Google Scholar
  25. Yurchenco PD, Furthmayr H (1984) Self-assembly of basement membrane collagen. Biochemistry 23:1839–1850Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Hitoshi Kubosawa
    • 1
  • Yoichiro Kondo
    • 1
  1. 1.Department of PathologySchool of Medicine, Chiba UniversityChibaJapan

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