Advertisement

Cell and Tissue Research

, Volume 242, Issue 3, pp 527–530 | Cite as

Immunohistochemical investigation of urotensins in the caudal spinal cord of four species of elasmobranchs and the lamprey, Lampetra japonica

  • Kyoko Owada
  • Chifumi Yamada
  • Hideshi Kobayashi
Article

Summary

In the four species of elasmobranchs examined (Triakis scyllia, Heterodontus japonicus, Scyliorhinus torazame, Dasyatis akajei), all identifiable caudal neurosecretory cells and their corresponding neurohemal areas showed urotensin II (UII)-immunoreactivity with varied intensity. To localize urotensin I (UI) in the caudal neurosecretory system of the dogfish, Triakis scyllia, h-CRF (1–20) antiserum that cross-reacts with UI was used in place of UI antiserum. CRF/UI-immunoreactivity was demonstrated in the neurosecretory cells and neurohemal areas. A considerable number of neurons showed both UII- and CRF/UI-immunoreactivities, suggesting that UII and UI are produced in the same neurosecretory cells. However, some neurons exhibited UII-immunoreactivity, but no CRF/UI-immunoreactivity. Cells immunoreactive only to CRF antiserum were not detected. At least two populations of neurons exist in the dogfish caudal neurosecretory system: (i) cells immunoreactive for both CRF/UI and UII, and (ii) cells immunoreactive for UII. The dorsal cells of the lamprey, Lampetra japonica, did not react with either UII or CRF antiserum.

Key words

Caudal neurosecretory system Urotensin II CRF Immunohistochemistry Elasmobranchs Cyclostomes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bern HA, Pearson D, Larson BA, Nishioka RS (1985) Neurohormones from fish tails — the caudal neurosecretory system I. “ Urophysiology ” and the caudal neurosecretory system of fishes. Recent Prog Horm Res 41: (in press)Google Scholar
  2. Fisher AWF, Wong K, Gill V, Lederis K (1984) Immunocytochemical localization of urotensin I neurons in the caudal neurosecretory system of the white sucker (Catostomus commersoni). Cell Tissue Res 235:19–23Google Scholar
  3. Fridberg G (1962) The caudal neurosecretory system in some elasmobranchs. Gen Comp Endocrinol 2:249–265Google Scholar
  4. Fridberg G, Bern HA (1968) The urophysis and the caudal neurosecretory system of fishes. Biol Rev 43:175–199Google Scholar
  5. Ichikawa T, McMaster D, Lederis K, Kobayashi H (1982) Isolation and amino acid sequence of urotensin I, a vasoactive and ACTH-releasing neuropeptide, from the carp (Cyprinus carpio) urophysis. Peptides 3:859–867Google Scholar
  6. Ichikawa T, Lederis K, Kobayashi H (1984) Primary structure of multiple forms of urotensin II in the urophysis of the carp, Cyprinus carpio. Gen Comp Endocrinol 55:133–141Google Scholar
  7. Ishimura K, Lederis K (1983) Immunohistochemistry of the caudal neurosecretory system in the sucker Catostomus commersoni. Folia Endocrinol Jpn 59:354Google Scholar
  8. Kobayashi H, Owada K, Okawara Y (1985) Morphology of the caudal neurosecretory system in the fish. In: Pang KT, Shreibman MP (eds) Vertebrate endocrinology: Fundamentals and biomedical applications. I Morphological considerations. Academic Press, New York (in press)Google Scholar
  9. Kriebel RM, Parsons RL, Miller KE (1985) Innervation of caudal neurosecretory cells. In: Kobayashi H, Bern HA, Urano A (eds) Neurosecretion. Jpn Sci Soc Press, Tokyo/Springer, Berlin, 457–463Google Scholar
  10. Larson BA, Nishioka RS, Pearson D, Bern HA (1984) Urotensins I and II: Presence in the same or different neurons of a fish caudal neurosecretory system? Am Zool 24:54AGoogle Scholar
  11. Lederis K, Letter A, McMaster D, Moore G, Schlesinger D (1982) Complete amino acid sequence of urotensin I, a hypotensive and corticotropin-releasing neuropeptide from Catostomus commersoni. Science 218:162–164Google Scholar
  12. McMaster D, Lederis K (1983) Isolation and amino acid sequence of two urotensin II pepetides form Catostomus commersoni urophyses. Peptides 4:367–373Google Scholar
  13. Onstott D, Elde R (1984) Immunohistochemical localization of urotensin I/corticotropin-releasing factor immunoreactivity in neurosecretory neurons in the caudal spinal cord of fish. Neuroendocrinology 39:503–509Google Scholar
  14. Owada K, Kobayashi H (1984) Urotensin II-immunoreactive neurons in the caudal neurosecretory system of the carp and the dogfish. Zool Sci 1:964Google Scholar
  15. Owada K, Kawata M, Akaji K, Takagi A, Moriga M, Kobayashi H (1985) Urotensin II-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish. Cell Tissue Res 239:349–354Google Scholar
  16. Pearson D, Shively JE, Clark BR, Geschwind II, Barkley M, Nishioka RS, Bern HA (1980) Urotensin II: A somatostatinlike peptide in the caudal neurosecretory system of fishes. Proc Natl Acad Sci, USA 77:5021–5024Google Scholar
  17. Peyrot A (1964) II sistema neurosecernente caudale dogli ittiopsidi: osservazioni sulla lampreda di ruscello (Lampetra zanandrei Vladikov). Bull Soc Ital Biol 40:207–211Google Scholar
  18. Renda T, D'Este L, Negri L, Lomoanto D (1982) Sauvagine-like immunoreactivity in the bony fish urophysis and caudal neurosecretory system. Bas Appl Histochem 26:89–98Google Scholar
  19. Sterba G (1962) Distribution of nerve cells with secretory-like granules in Petromyzontes. Nature 193:400–401Google Scholar
  20. Sternberger LA, Hardy PH, Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Kyoko Owada
    • 1
  • Chifumi Yamada
    • 1
  • Hideshi Kobayashi
    • 1
  1. 1.Department of Biology, Faculty of ScienceToho UniversityChibaJapan

Personalised recommendations