Cell and Tissue Research

, Volume 242, Issue 3, pp 527–530 | Cite as

Immunohistochemical investigation of urotensins in the caudal spinal cord of four species of elasmobranchs and the lamprey, Lampetra japonica

  • Kyoko Owada
  • Chifumi Yamada
  • Hideshi Kobayashi


In the four species of elasmobranchs examined (Triakis scyllia, Heterodontus japonicus, Scyliorhinus torazame, Dasyatis akajei), all identifiable caudal neurosecretory cells and their corresponding neurohemal areas showed urotensin II (UII)-immunoreactivity with varied intensity. To localize urotensin I (UI) in the caudal neurosecretory system of the dogfish, Triakis scyllia, h-CRF (1–20) antiserum that cross-reacts with UI was used in place of UI antiserum. CRF/UI-immunoreactivity was demonstrated in the neurosecretory cells and neurohemal areas. A considerable number of neurons showed both UII- and CRF/UI-immunoreactivities, suggesting that UII and UI are produced in the same neurosecretory cells. However, some neurons exhibited UII-immunoreactivity, but no CRF/UI-immunoreactivity. Cells immunoreactive only to CRF antiserum were not detected. At least two populations of neurons exist in the dogfish caudal neurosecretory system: (i) cells immunoreactive for both CRF/UI and UII, and (ii) cells immunoreactive for UII. The dorsal cells of the lamprey, Lampetra japonica, did not react with either UII or CRF antiserum.

Key words

Caudal neurosecretory system Urotensin II CRF Immunohistochemistry Elasmobranchs Cyclostomes 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Kyoko Owada
    • 1
  • Chifumi Yamada
    • 1
  • Hideshi Kobayashi
    • 1
  1. 1.Department of Biology, Faculty of ScienceToho UniversityChibaJapan

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