Theoretical and Applied Genetics

, Volume 85, Issue 6–7, pp 873–881 | Cite as

Abnormal macrosporogenesis in five alfalfa (Medicago sativa) mutants producing 4n pollen

  • A. Mariani
  • S. Tavoletti
  • F. Veronesi
Originals

Summary

An analysis of micro- and macrosporogenesis in five diploid alfalfa mutants was carried out using a stain-clearing technique. All plants produced tetranucleated microspores and jumbo pollen due to the complete failure of the postmeiotic cytokinesis as well as bi- and trinucleated macrospores. The latter was due to the absence of cytokinesis after the first and second meiotic division of macrosporogenesis. Only one out of the five clones analyzed formed tetranucleated macrospores as a consequence of the total lack of cytokinesis after both meiotic divisions. The fusion of nuclei within binucleated macrospores resulted in 2n macrospores of the SDR type, recognizable on the basis of nucleolus dimension, confirming the ability of jumbo pollen (jp) mutants to produce 2n eggs at a high frequency. Nuclear fusion was also observed within tri- and tetranucleated macrospores. Although having the same genetic background, the five clones showed significant variability in the expression of abnormal cytokinesis during macrosporogenesis.

Key words

Reproductive mutants Jumbo pollen Alfalfa Cytogenetics Sexual polyploidization 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Mariani
    • 1
  • S. Tavoletti
    • 2
  • F. Veronesi
    • 2
  1. 1.Istituto di Ricerche sul Miglioramento Genetico delle Plante Foraggere, C.N.R.PerugiaItaly
  2. 2.Dipartimento di Biotecnologie Agrarie e Ambientali, Facoltá di AgrariaUniversitá degli Studi di AnconaAnconaItaly

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