Advertisement

Theoretical and Applied Genetics

, Volume 85, Issue 6–7, pp 801–808 | Cite as

Molecular analysis of highly repeated genome fractions in Solanum and their use as markers for the characterization of species and cultivars

  • G. Schweizer
  • N. Borisjuk
  • L. Borisjuk
  • M. Stadler
  • T. Stelzer
  • L. Schilde
  • V. Hemleben
Originals

Summary

Highly repeated DNA of potato (Solanum sp.) was characterized by cloning various major repeated elements of the nuclear genome. The percentage of the nuclear genome of the specific fractions and the restriction enzyme patterns were determined in order to show the distribution and organization of the respective repeats in the genome of Solanum tuberosum cultivars, dihaploid breeding lines and in wild species of Solanum. Several of the clones obtained were represented in a high copy number but showed no informative RFLP patterns. More information was gained from ‘restriction satellite’ repeats. The clone pR1T320 was found to contain satellite repeats (360 bp in length) that are proportionally present in the genome of all Solanum species at frequencies, between 0.5% and 2.6% and which are differently organized. This repeat was also found in the genera Lycopersicon, Datura and Nicotiana. With various restriction enzymes characteristic RFLP patterns were detected. A more or less genus-specific element for Solanum was the 183-bp repeat (clone pSA287; between 0.2–0.4% of the nuclear genome) that was present in the majority of the Solanum species analyzed except S. kurtzianum, S. bulbocastanum and S. pinnatisectum. In a few wild species (prominently in S. kurtzianum, S. demissum and S. acaule) a specific repeat type was detected (clone pSDT382; repeat length approximately 370 bp) that could be used to trace the wild species introduced into S. tuberosum cultivars. The repeats analyzed together with the 18S, 5.8S and 25S ribosomal DNA (1.9–5.2%, corresponding to 1800-5500 rDNA copies) comprised approximately 4–7% of the Solanum genome.

Key words

Repeated DNA Restriction satellite RFLP Solanaceae Species-specificity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218Google Scholar
  2. Birnboim HC, Doly I (1979) A rapid alkaline extraction for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523PubMedGoogle Scholar
  3. Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103Google Scholar
  4. Bostock C (1986) Mechanisms of DNA sequence amplification and their evolutionary consequences. Philos Trans R Soc London 312:261–273Google Scholar
  5. Debener T, Salamini F, Gebhardt C (1990) Phytogeny of wild and cultivated Solanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 79:360–368Google Scholar
  6. Feinberg A, Vogelstein B (1983) A technique for radio-labelling DNA restriction fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  7. Flavell RB (1985) Repeated sequences and genome change. In: Hohn B, Dennis E (eds) Genetic flux in plants. Springer, Berlin Heidelberg, New York, pp 139–156Google Scholar
  8. Ganal MW, Tanksley SD (1989) Analysis of tomato DNA by pulsed field gel electrophoresis. Plant Mol Biol Rep 7:17–27Google Scholar
  9. Ganal M, Riede I, Hemleben V (1986) Organization and sequence analysis of two related satellite DNAs in cucumber (Cucumis sativus). J Mol Evol 23:23–30Google Scholar
  10. Ganal MW, Lapitan NLV, Tanksley SD (1988) A molecular and cytogenetic survey of major repeated DNA sequences in tomato (Lycopersicon esculentum). Mol Gen Genet 213:262–268Google Scholar
  11. Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75Google Scholar
  12. Gruber V (1991) Heterozygosity in tetraploid potatoes revealed by rDNA polymophism analysis of their dihaploid progenies: a contribution to chromosome assignment. Plant Mol Biol 17:1045–1054Google Scholar
  13. Grunstem M, Hogness D (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965Google Scholar
  14. Hawkes JG (1990) The potato evolution, biodiversity and genetic resources. Smithsonian Institution Press, Washington, D.C.Google Scholar
  15. Hemleben V, Leweke B, Roth A, Stadler I (1982) Organization of highly repeated satellite DNA of two Cucurbitaceae species (Cucumis melo and Cucumis sativus). Nucleic Acids Res 10:631–644Google Scholar
  16. Hemleben V, Zentgraf U, King K, Borisjuk N, Schweizer G (1992) Middle repetitive and highly repetitive sequences detect polymorphisms in plants. In: Kahl G, Appelhans H, Kömpf J, Driesel AJ (eds) DNA polymorphisms in eucaryotic genomes. Hüthig, Heidelberg, pp 157–170Google Scholar
  17. Hosaka K (1986) Who is the mother of the potato? — restriction endonuclease analysis of chloroplast DNA of cultivated potatoes. Theor Appl Genet 72:606–618Google Scholar
  18. Jung C, Kleine M, Fischer F, Herrmann RG (1990) Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672Google Scholar
  19. Koukalova B, Reich J, Matyasek R, Kuhrova V, Bezdek M (1989) A BamHI family of repeated DNA sequences of Nicotiana tabacum. Theor Appl Genet 78:77–80Google Scholar
  20. Kuhrova V, Bezdek M, Vyskot B, Koukalova B, Fajkus J (1991) Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genome. Theor Appl Genet 81:740–744Google Scholar
  21. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  22. Metzlaff M, Troebner W, Baldauf F, Schlegel R, Cullum J (1986) Wheat-specific repetitive DNA sequences — construction and characterization of four different genomic clones. Theor Appl Genet 72:207–210Google Scholar
  23. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  24. Pech M, Igo-Kemenes T, Zachau HG (1979) Nucleotide sequence of a highly repetitive component of rat DNA. Nucleic Acids Res 7:417–432Google Scholar
  25. Pehu E, Thomas M, Poutala T, Karp A, Jones MGK (1990) Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum. Theor Appl Genet 80:693–698Google Scholar
  26. Ross H (1986) Potato breeding — problems and perspectives. Advances in plant breeding. J Plant Breed [Suppl 13]Google Scholar
  27. Schilde-Rentschler L, Doos G, Ninnemann H (1987) Somatic hybridization of dihaploid potato breeding lines, a tool in potato breeding. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornef M, Krens FA (eds) Current plant science and biotechnology in agriculture: progress in protoplast research. Kluwer Academic Publ, Dordrecht Boston London, pp 195–196Google Scholar
  28. Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor Appl Genet 75:679–684Google Scholar
  29. Schweizer G, Stelzer T, Hemleben V (1990) RFLP-Analyse mit spezifischen Genomkomponenten der Kartoffel zur Identifikation von symmetrischen und asymmetrischen Hybriden. Vortr Pflanzenzuecht 18:178–192Google Scholar
  30. Shure M, Wessler S, Fedoroff N (1983) Molecular identification and isolation of the waxy locus in maize. Cell 35:225–233CrossRefPubMedGoogle Scholar
  31. Torres RA (1985) Struktur, Sequenzhomogenität und Methylierung der ribosomalen RNA-Gene von Cucurbita pepo L. Diplomarbeit, Universität TübingenGoogle Scholar
  32. Wenzel W, Hemleben V (1982) A comparative study of genomes in angiosperms. Plant Syst Evol 139:209–227Google Scholar
  33. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13 mp 18 and pUC 19 vectors. Gene 33:103–199CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • G. Schweizer
    • 1
  • N. Borisjuk
    • 3
  • L. Borisjuk
    • 3
  • M. Stadler
    • 3
  • T. Stelzer
    • 3
  • L. Schilde
    • 2
  • V. Hemleben
    • 3
  1. 1.Bayrische Landesanstalt für Bodenkultur und Pflanzenbau, PZ 1.3 ‘Biotechnologie’FreisingGermany
  2. 2.Medizinisch-Naturwissenschaftliches Zentrum der Universität TübingenTübingenGermany
  3. 3.Lehrstuhl für Allgemeine GenetikBiologisches Institut, Universität TübingenTübingenGermany

Personalised recommendations