Theoretical and Applied Genetics

, Volume 85, Issue 6–7, pp 729–734 | Cite as

Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens

  • Y. S. Xu
  • M. Murto
  • R. Dunckley
  • M. G. K. Jones
  • E. Pehu


Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.

Key words

Protoplast fusion Gamma irradiation Partial genome transfer Solanum tuberosum Solanum brevidens 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet 74:718–726Google Scholar
  2. de Vries SE, Jacobsen E, Jones MGK, Loonen AEHM, Tempelaar MJ, Wijbrandi J, Feenstra WJ (1987) Somatic hybridisation of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion. Theor Appl Genet 73:451–458Google Scholar
  3. Draper J, Scott R, Armitage P, Walden R (1988) Plant genetic transformation and gene expression. A laboratory manual. Black well Scientific Publ London, pp 2112–2114Google Scholar
  4. Dudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey J, Cella R (1987) Transfer of resistance traits from carrot into tobacco by asymmetric hybridization: regeneration of fertile plants. Proc Natl Acad Sci USA 84:8434–8438Google Scholar
  5. Dudits D, Fejer O, Hadlaczky GY, Koncz CS, Lazar G, Horvath G (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288Google Scholar
  6. Ehlenfeldt MK, Helgeson JP (1987) Fertility of somatic hybrids from protoplast fusion of Solanum brevidens and S. tuberosum. Theor Appl Genet 73:395–402Google Scholar
  7. Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parkonny AS, Boryshuk NY, Cherup NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids between Nicotiana plum-baginifolia and Nicotiana sylvestris obtained by ‘gamma fusion’. Plant Sci 61:105–117Google Scholar
  8. Fish N, Karp A, Jones MGK (1987) Improved isolation of dihaploid Solanum tuberosum protoplasts and the production of somatic hybrids between dihaploid S. ttuberosum and S. Brevidens. In Vitro Cell Dev biol 23:575–580Google Scholar
  9. Fish N, Karp A, Jones MGK (1988a) Production of somatic hybrids by electrofusion in Solanum. Theor Appl Genet 76:260–266Google Scholar
  10. Fish N, Steele SH, Jones MGK (1988 b) Field assessment of dihaploid S. tuberosum and S. brevidens somatic hybrids. Theor Appl Genet 76:880–886Google Scholar
  11. Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherup NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plum-baginifolia and Atropa belladonna obtained by ‘gammafusion’. Theor Appl Genet 76:760–766Google Scholar
  12. Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet 197:30–35Google Scholar
  13. Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272Google Scholar
  14. Jones MGK, Dunckley R, Steele S, Karp A, Gibson R, Fish N, Valkonen J, Poutala T, Pehu E (1990) Transfer of resistance to PLRV, PVX and PVY from S. brevidens to potato by somatic hybridization: chracterization and field evaluation. In: Nijkamp HJJ, Vanderplas LHW, Vanaartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer Academic Publ, Dordrecht Boston London, pp 286–292Google Scholar
  15. Murashiga T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  16. National Institute of Agricultural Botany (1975) Guide to the identification of potato varieties. Cambridge University Press, LondonGoogle Scholar
  17. Pehu E, Thomas M, Poutala T, Karp A, Jones MGK (1990) Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum. Theor Appl Genet 80:693–698Google Scholar
  18. Ramanna MS, Hermsen JG (1981) Structural hybridity in the series Etuberosa of the genus Solanum and its bearing on crossability. Euphytica 30:15–31Google Scholar
  19. Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybridization of tomatoes by ‘gamma-fusion’ of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. ‘dentatum’ Dun. protoplasts. Plant Sci 73:65–78Google Scholar
  20. Sidorov V, Zubko MK, Kuchko AA, Komarnitsky IK, Gleba YY (1987) Somatic hybridization in potato: use of gamma-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction. Theor Appl Genet 74:364–368Google Scholar
  21. Valkonen JPT, Brigneti G, Salazar LF, Pehu E, Gibson RW (1992) Interactions of the Solanum spp. of the etuberosa group and nine potato-infecting virus and a viroid. Ann Appl Biol 120:301–313Google Scholar
  22. Wijbrandi J, Posthuma A, Kok RR, Vos JGM, Koornneef M (1990a) Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum. 1. Cytogenetics and morphology. Theor Appl Genet 80:305–312Google Scholar
  23. Wijbrandi J, Wolters AMA, Koornneef M (1990 b) Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peuvianum. 2. Analysis with marker genes. Theor Appl Genet 80:665–672Google Scholar
  24. Yamashita Y, Terada R, Nishibayashi S, Shimamoto K (1989) Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion. Theor Appl Genet 77:189–194Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Y. S. Xu
    • 1
  • M. Murto
    • 1
  • R. Dunckley
    • 2
  • M. G. K. Jones
    • 3
  • E. Pehu
    • 1
  1. 1.Department of Plant ProductionUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Biochemistry and PhysiologyRothamsted Experimental StationHarpendenUK
  3. 3.School of Biological and Environmental Sciences, Murdoch UniversityPerth

Personalised recommendations