Theoretical and Applied Genetics

, Volume 85, Issue 6–7, pp 729–734 | Cite as

Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens

  • Y. S. Xu
  • M. Murto
  • R. Dunckley
  • M. G. K. Jones
  • E. Pehu
Originals

Summary

Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.

Key words

Protoplast fusion Gamma irradiation Partial genome transfer Solanum tuberosum Solanum brevidens 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Y. S. Xu
    • 1
  • M. Murto
    • 1
  • R. Dunckley
    • 2
  • M. G. K. Jones
    • 3
  • E. Pehu
    • 1
  1. 1.Department of Plant ProductionUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Biochemistry and PhysiologyRothamsted Experimental StationHarpendenUK
  3. 3.School of Biological and Environmental Sciences, Murdoch UniversityPerth

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