Theoretical and Applied Genetics

, Volume 80, Issue 2, pp 183–187 | Cite as

Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin

2. Genetic control of the subunits in species related to wheat
  • R. B. Gupta
  • K. W. Shepherd


Analysis of intergeneric substitution lines in hexaploid wheats by a two-step electrophoretic method of protein separation revealed that low-molecular-weight (LMW) subunits of glutelin in Triticum longissimum, T. Umbelullatum, Elytrigia elongata (2 x) were controlled by chromosomes/chromosome arms 1S l , 1U, and 1ES, respectively. A LMW glutelin band in Secale montanum was detected but its chromosomal location could not be determined. Genes controlling gliadins and HMW subunits of glutelin were also located on chromosome 1S l in T. longissimum.

Key words

Triticum longissimum T. umbelullatum Elytrigia elongata LMW subunits of glutelin Chromosomal location 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Athwal RS, Kimber G (1972) The pairing of an alien chromosome with homoeologous chromosomes of wheat. Can J Genet Cytol 14:325–333Google Scholar
  2. Autran J-C, Laignelet B, Morel MH (1987) Characterization and quantification of low-molecular-weight glutenins in durum wheats. Biochimie 69:699–711Google Scholar
  3. Bartels D, Altosaar I, Harberd NP, Barker RF, Thompson RD (1986) Molecular analysis of γ-gliadin gene families at the complex Gli-1 locus of bread wheat (T. aestivum L.). Theor Appl Genet 72:845–853Google Scholar
  4. Colot V, Bartels D, Thompson R, Flavell R (1989) Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes. Mol Gen Genet 216:81–90Google Scholar
  5. Dvorak J (1980) Homoeology between Agropyron elongatum chromosomes and Triticum aestivum chromosomes. Can J Genet Cytol 22:237–259Google Scholar
  6. Dvorak J (1981) Genome relationships among Elytrigia (=Agropyron) elongata, E. stipifolia, “E. elongata 4x”, E. caespitosa, E. intermedia, and “E. elongata 10 x”. Can J Genet Cytol 23:481–492Google Scholar
  7. Dvorak J, Sosulski FW (1974) Effects of additions and substitutions of Agropyron elongatum chromosomes on quantitative characters in wheat. Can J Genet Cytol 16:627–637Google Scholar
  8. Gupta RB (1989) Low-molecular-weight subunits of glutenin in wheat and related species: their characterization, genetics, and relation to bread-making quality. PhD Thesis, University of Adelaide, AustraliaGoogle Scholar
  9. Gupta RB, Shepherd KW (1987) Genetic control of LMW glutenin subunits in bread wheat and association with physical dough properties. In: Proc 3rd Int Gluten Workshop, Budapest, Hungary. World Scientific Publishers, Singapore, pp 13–19Google Scholar
  10. Gupta RB, Shepherd KW (1988) Low-molecular-weight glutenin subunits in wheat: their variation, inheritance, and association with bread-making quality. In: Proc 7th Int Wheat Genet Symp, Cambridge, England, pp 943–949Google Scholar
  11. Gupta RB, Shepherd KW (1990) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet 80:65–74Google Scholar
  12. Gupta RB, Singh NK, Shepherd KW (1989a) The cumulative effect of allelic variation in LMW and HMW glutenin subunits on physical dough properties in progeny of two bread wheats. Theor Appl Genet 77:57–64Google Scholar
  13. Gupta RB, Shepherd KW, MacRitchie F (1989b) Effects of rye chromosome arm 2RS on flour proteins and physical dough properties in bread wheat. J Cereal Sci 10:169–173Google Scholar
  14. Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126Google Scholar
  15. Jackson EA, Holt LM, Payne PI (1983) Characterization of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal location of their controlling genes. Theor Appl Genet 66:29–37Google Scholar
  16. Kasarda DD, Tao HP, Evans PK, Adalsteins AE, Yuen SW (1988) Sequencing of protein from a single spot of a 2-D gel pattern: N-terminal sequence of a major wheat LMW-glutenin subunit. J Exp Bot 39:899–906Google Scholar
  17. Kimber G (1967) The addition of the chromosomes of Aegilops umbellulata to Triticum aestivum (var. Chinese Spring). Genet Res 9:111–114Google Scholar
  18. Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley, and rye. Oxford Surv Plant Mol Cell Biol 2:253–317Google Scholar
  19. Lawrence GJ, Shepherd KW (1981) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31Google Scholar
  20. Morris R, Sears ER (1967) The cytogenetics of wheat and its relatives. In: Quisenberry KS, Reitz LP (eds) Wheat and wheat improvement. ASA, Madison/WIGoogle Scholar
  21. Netzle S, Zeller FJ (1984) Cytogenetic relationship of Aegilops longissima chromosomes with common wheat chromosomes. Plant Syst Evol 145:1–13PubMedGoogle Scholar
  22. Okita TW, Cheesbrough V, Reeves CD (1985) Evolution and heterogeneity of the α-/β-type and γ-type gliadin DNA sequences. J Biol Chem 260:8203–8213Google Scholar
  23. Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153Google Scholar
  24. Rogers WJ, Payne PI, Miller TE, Holt LM, Law CN, Sayers EJ, Seekings JA (1989) Introduction to hexaploid wheat and assessment for bread-making quality of a Glu-Al locus from Triticum thaoudar encoding two high-molecular-weight subunits of glutenin. In: Proc 12th Eucarpia Congr, Göttingen, FRG, p 15Google Scholar
  25. Shepherd KW (1973) Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Proc 4th Int Wheat Genet Symp. Columbia/MO, pp 745–760Google Scholar
  26. Singh NK, Shepherd KW (1988) Linkage mapping of the genes controlling endosperm proteins in wheat. 1. Genes on the short arms of group 1 chromosomes. Theor Appl Genet 75:628–641Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • R. B. Gupta
    • 1
  • K. W. Shepherd
    • 1
  1. 1.Department of Agronomy, Waite Agricultural Research InstituteUniversity of AdelaideGlen OsmondAustralia

Personalised recommendations