Theoretical and Applied Genetics

, Volume 93, Issue 5–6, pp 869–876 | Cite as

Development of simple sequence repeat DNA markers and their integration into a barley linkage map

  • Z. -W. Liu
  • R. M. Biyashev
  • M. A. Saghai Maroof


Simple sequence repeats (SSRs), or microsatellites, are a new class of PCR-based DNA markers for genetic mapping. The objectives of the present study were to develop SSR markers for barley and to integrate them into an existing barley linkage map. DNA sequences containing SSRs were isolated from a barley genomic library and from public databases. It is estimated that the barley genome contains one (GA)n repeat every 330 kb and one (CA)n repeat every 620 kb. A total of 45 SSRs were identified and mapped to seven barley chromosomes using doubled-haploid lines and/or wheat-barley addition-line assays. Segregation analysis for 39 of these SSRs identified 40 loci. These 40 markers were placed on a barley linkage map with respect to 160 restriction fragment length polymorphism (RFLP) and other markers. The results of this study demonstrate the value of SSRs as markers in genetic studies and breeding research in barley.

Key words

Microsatellites Hordeum vulgare Molecular marker Linkage map 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Z. -W. Liu
    • 1
  • R. M. Biyashev
    • 1
  • M. A. Saghai Maroof
    • 1
  1. 1.Department of Crop and Soil Environmental SciencesVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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