Theoretical and Applied Genetics

, Volume 92, Issue 8, pp 1031–1037 | Cite as

Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.)

  • V. Srivastava
  • V. Vasil
  • I. K. Vasil


Molecular analysis of the transgenes bar and gus was carried out over successive generations in six independent transgenic lines of wheat, until the plants attained homozygosity. Data on expression and integration of the transgenes is presented. Five of the lines were found to be stably transformed, duly transferring the transgenes to the next generation. The copy number of the transgenes varied from one to five in the different lines. One line was unstable, first losing expression of and then eliminating both the transgenes in R3 plants. Although the gus gene was detected in all the lines, GUS expression had been lost in R2 plants of all but one line. Rearrangement of transgene sequences was observed, but it had no effect on gene expression. All the stable lines were found to segregate for transgene activity in a Mendelian fashion.

Key words

Triticum aestivum L. Transformation Transgene inheritance Wheat 


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  1. Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant Jour 5:299–307Google Scholar
  2. Castillo AM, Vasil V, Vasil IK (1994) Rapid production of fertile transgenic plants of rye (Secale cereale L.). Bio/Technol 12:1366–1371Google Scholar
  3. Cedar H (1988) DNA methylation and gene activity. Cell 53:3–4Google Scholar
  4. Christensen AH, Sharrock RA, Quail PH (1992) Maize ubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol 18:675–689PubMedGoogle Scholar
  5. Cooley J, Ford T, Christou P (1995) Molecular and genetic characterization of elite transgenic rice plants produced by electric discharge particle acceleration. Theor Appl Genet 90:97–104Google Scholar
  6. Finnegan J, McElroy D (1994) Transgene inactivation: plants fight back! Bio/Technol 12:883–888Google Scholar
  7. Flavell RB (1994) Inactivation of gene expression in plants as a consequence of specific gene duplication. Proc Natl Acad Sci USA 91:3490–3496PubMedGoogle Scholar
  8. Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimeric genes in the progenies of transgenic maize plants. Bio/Technol 8:833–839Google Scholar
  9. Goto F, Toki S, Uchimiya H (1993) Inheritance of a co-transferred foreign gene in the progenies of transgenic rice plants. Transgenic Res 2:300–305Google Scholar
  10. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405Google Scholar
  11. Lassner NW, Peterson P, Yoder JI (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mol Biol Rep 7:116–128Google Scholar
  12. Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Jour 5:285–297Google Scholar
  13. Register III JC, Peterson DJ, Bell PJ, Bullock WP, Evans BF, Greenland AJ, Higgs NS, Jepson I, Jiao S, Lewnau CJ, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951–961Google Scholar
  14. Spencer TM, O'Brien JV, Start WG, Adams TR, Gordan-Kamm WJ, Lemaux PG (1992) Segregation of transgenes in maize. Plant Mol Biol 18:201–210Google Scholar
  15. Vasil IK (1994) Molecular improvement of cereals. Plant Mol Biol 25:925–937Google Scholar
  16. Vasil V, Castillo AM, Fromm ME, Vasil I K (1992) Herbicideresistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Biol/Technol 10:667–674Google Scholar
  17. Vasil V, Srivastava V, Castillo AM, Fromm ME, Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technol 11:1553–1558Google Scholar
  18. Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077–1084Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • V. Srivastava
    • 1
  • V. Vasil
    • 1
  • I. K. Vasil
    • 1
  1. 1.Laboratory of Plant Cell and Molecular BiologyUniversity of FloridaGainesvilleUSA

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