Theoretical and Applied Genetics

, Volume 91, Issue 6–7, pp 1092–1094

Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

  • A. Lehner
  • M. A. Campbell
  • N. C. Wheeler
  • T. Pöykkö
  • J. Glössl
  • J. Kreike
  • D. B. Neale
Article

DOI: 10.1007/BF00223924

Cite this article as:
Lehner, A., Campbell, M.A., Wheeler, N.C. et al. Theoret. Appl. Genetics (1995) 91: 1092. doi:10.1007/BF00223924

Abstract

The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SEr=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.

Key words

Norway spruce Pendula phenotype Bulked segregant analysis RAPD markers 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. Lehner
    • 1
  • M. A. Campbell
    • 2
  • N. C. Wheeler
    • 3
  • T. Pöykkö
    • 4
  • J. Glössl
    • 5
  • J. Kreike
    • 1
  • D. B. Neale
    • 6
  1. 1.Institute of Forensic MedicineViennaAustria
  2. 2.Division of SciencePenn State Erie, The Behrend CollegeErieUSA
  3. 3.Weyerhaeuser CompanyCentraliaUSA
  4. 4.Foundation of Forest Tree BreedingHelsinkiFinland
  5. 5.Zentrum für angewandte Genetik der Universität für Bodenkultur WienViennaAustria
  6. 6.USDA/Forest Service, Institute of Forest GeneticsBerkeleyUSA

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