Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)
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- Lehner, A., Campbell, M.A., Wheeler, N.C. et al. Theoret. Appl. Genetics (1995) 91: 1092. doi:10.1007/BF00223924
The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SEr=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.
Key wordsNorway spruce Pendula phenotype Bulked segregant analysis RAPD markers
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