Advertisement

Theoretical and Applied Genetics

, Volume 91, Issue 6–7, pp 1001–1007 | Cite as

Detection of genetic diversity in closely related bread wheat using microsatellite markers

  • J. Plaschke
  • M. W. Ganal
  • M. S. Röder
Article

Abstract

Wheat microsatellites (WMS) were used to estimate the extent of genetic diversity among 40 wheat cultivars and lines, including mainly European elite material. The 23 WMS used were located on 15 different chromosomes, and revealed a total of 142 alleles. The number of alleles ranged from 3 to 16, with an average of 6.2 alleles per WMS. The average dinucleotide repeat number ranged from 13 to 41. The correlation coefficient between the number of alleles and the average number of repeats was only slight (rs = 0.55). Based on percentage difference a dendrogram is presented, calculated by the WMS-derived data. All but two of the wheat cultivars and lines could be distinguished. Some of the resulting groups are strongly related to the pedigrees of the appropriate cultivars. Values for co-ancestry (f) of 179 pairs of cultivars related by their pedigrees (f⩾0.1) averaged 0.29. Genetic similarity (GS) based on WMS of the same pairs averaged 0.44. The rank correlation for these pairs was slight, with rs = 0.55, but highly significant (P<0.001). The results suggest that a relatively small number of microsatellites can be used for the estimation of genetic diversity and cultivar identification in elite material of hexaploid bread wheat.

Key words

Co-ancestry Genetic diversity Microsatellites Wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chen HB, Martin JM, Lavin M, Talbert LE (1994) Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci 34:1629–1632Google Scholar
  2. Cox TS, Lookhart GL, Walker DE, Harrell LG, Albers LD, Rodgers DM (1985) Genetic relationships among hard red winter wheat cultivars as evaluated by pedigree analysis and gliadin polyacrylamide-gel electrophoretic patterns. Crop Sci 25:1058–1063Google Scholar
  3. Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252Google Scholar
  4. Dweikat I, MacKenzie S, Levy M, Ohm H (1993) Pedigree assessment using RAPD-DGGE in cereal crop species. Theor Appl Genet 83:497–505Google Scholar
  5. Graner A, Ludwig WF, Melchinger AE (1995) Relationships among European barley germplasm. II. Comparison of RFLP and pedigree data. Crop Sci 34:1199–1205Google Scholar
  6. He S, Ohm H, Mackenzie S (1992) Detection of DNA sequence polymorphisms among wheat varieties. Theor Appl Genet 84:573–578Google Scholar
  7. Levinson G, Gutman GA (1987) Slipped strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4:203–221PubMedGoogle Scholar
  8. Martynov SP, Dobrotvorskaya TV, Stehno Z, Dotlacil L, Faberova I, Holubec V (1992) Catalogue — genealogies and gene alleles identified in 31000 cultivars and lines of wheat. Research Institute of Crop Production, PragueGoogle Scholar
  9. Melchinger AE, Graner A, Singh M, Messmer MM (1995) Relationships among European barley germplasm I. Genetic diversity among winter and spring cultivars revealed by RFLPs. Crop Sci 34:1191–1199Google Scholar
  10. Murphy GL, Connell TD, Barritt DS, Koomey M, Cannon JG (1989) Phase variation of gonococcal protein. II. Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence. Cell 56:539–547Google Scholar
  11. Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273PubMedGoogle Scholar
  12. Plaschke J, Börner A, Wendehake K, Ganal MW, Röder MS (1995) The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica (in press)Google Scholar
  13. Podani J (1990) SYN-TAX III-pc-supplement3: Macintosh version. Abstr Bot 14:23–29Google Scholar
  14. Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333PubMedGoogle Scholar
  15. Rongwen J, Akkaya MS, Bhagwat AA, Lavi U, Cregan PB (1995) The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90:43–48Google Scholar
  16. Saghai Maroof MA, Biyashev RM, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA 91:5466–5470PubMedGoogle Scholar
  17. Scarth R (1981) The genetic control of daylength response in wheat. Ph. D thesis, The University of CambridgeGoogle Scholar
  18. Sears ER (1966) Nuilisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulation and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45Google Scholar
  19. Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proc 5th Int Wheat Genet Symp. Indian Soc Genet Plant Breed, New Delhi, pp 389–407Google Scholar
  20. Sheen SJ, Snyder LA (1964) Studies on the inheritance of resistance to six stem rust cultures using chromosome substitution lines of a Marquis wheat selection. Can J Genet Cytol 6:74–82Google Scholar
  21. Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794Google Scholar
  22. Tinker NA, Fortin MG, Mather DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet 85:976–984Google Scholar
  23. Tautz D, Schlötterer C (1994) Simple sequences. Curr Opin Genet Dev 4:832–837Google Scholar
  24. Vaccino P, Accerbi M, Corbellini M (1993) Cultivar identification in T. aestivum using highly polymorphic RFLP probes. Theor Appl Genet 86:833–836Google Scholar
  25. Viglasi P (1968) Short-strawed mutants of Karcag 522 winter wheat induced by gamma rays. Acta Agron 17:205–214Google Scholar
  26. Weber JL (1990) Informativeness of human (dC-dA)n·(dG-dT)n polymorphisms. Genomics 7:524–530PubMedGoogle Scholar
  27. Wolff RK, Plaetke R, Jeffreys AJ, White R (1989) Unequal crossingover between homologous chromosomes is not the major mechanism involved in the generation of new alleles at VNTR loci. Genomics 5:382–384Google Scholar
  28. Ynag GP, Saghai Maroof MA, Xu CG, Qifa Zhang, Biyashev RM (1994) Comparative analysis of microsatellite DNA polymorphism in landraces and cultivars of rice. Mol Gen Genet 245:187–194PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • J. Plaschke
    • 1
  • M. W. Ganal
    • 1
  • M. S. Röder
    • 1
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany

Personalised recommendations