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Theoretical and Applied Genetics

, Volume 91, Issue 6–7, pp 964–971 | Cite as

A linkage map with RFLP and isozyme markers for almond

  • M. A. Viruel
  • R. Messeguer
  • M. C. de Vicente
  • J. Garcia-Mas
  • P. Puigdomènech
  • F. Vargas
  • P. Arús
Article

Abstract

Inheritance and linkage studies were conducted with seven isozyme genes and 120 RFLPs in the F1 progeny of a cross between almond cultivars ‘Ferragnes’ and ‘Tuono’. RFLPs were detected using 57 genomic and 43 cDNA almond clones. Eight of the cDNA probes corresponded to known genes (extensin, prunin (2), α-tubulin, endopolygalacturonase, oleosin, actin depolymerizing factor and phosphoglyceromutase). Single-copy clones were found more frequently in the cDNA (65%) than in the genomic libraries (26%). Two maps were elaborated, one with the 93 loci heterozygous in ‘Ferragnes’ and another with the 69 loci heterozygous in ‘Tuono’. Thirty-five loci were heterozygous in both parents and were used as bridges between both maps. Most of the segregations (91%) were of the 1∶1 or 1∶1∶1∶1 types, and data were analyzed as if they derived from two backcross populations. Eight linkage groups covering 393 cM in ‘Ferragnes’ and 394 in ‘Tuono’ were found for each map. None of the loci examined in either map was found to be unlinked. Distorted segregation ratios were mainly concentrated in two linkage groups of the ‘Ferragnes’ map.

Key words

Almond Prunus amygdalus Isozymes RFLPs Genetic maps 

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References

  1. Ahn S, Tanskley SD (1993) Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci 90:7980–7984PubMedGoogle Scholar
  2. Arulsekar S, Parfitt DE, Kester DE (1986) Comparison of isozyme variability in peach and almond cultivars. J Hered 77:272–274Google Scholar
  3. Arús P, Olarte C, Romero M, Vargas F (1994a) Linkage analysis of ten isozyme genes in F1 segregating almond progenies. J Am Soc Hortic Sci 119:339–344Google Scholar
  4. Arús P, Messeguer R, Viruel MA, Tobutt K, Dirlewanger E, Santi F, Quarta R, Ritter E (1994b) The European Prunus mapping project. Progress in the almond linkage map. Euphytica 77:97–100Google Scholar
  5. Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898Google Scholar
  6. Burr B, Burr RA, Thompson KH, Albertsen MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526Google Scholar
  7. Byrne DH (1990) Isozyme varibility in four diploid stone fruits compared with other woody perennial plants. J Hered 81:68–71Google Scholar
  8. Cerezo M, Socias i Company R, Arús P (1989) Identification of almond cultivars by pollen isoenzymes. J Am Soc Hortic Sci 114:164–169Google Scholar
  9. Chaparro JX, Durham RE, Moore GA, Sherman WB (1987) Use of isozyme techniques to identify peach x ‘Nonpareil’ almond hybrids. HortScience 22:300–302Google Scholar
  10. Chaparro JX, Werner DJ, O'Malley D, Sederoff RR (1994) Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach. Theor Appl Genet 87:805–815Google Scholar
  11. Dickson EE, Arumuganathan K, Kresovich S, Doyle JJ (1992) Nuclear DNA content variation within the rosaceae. Am J Bot 79:1081–1086Google Scholar
  12. Eldredge L, Ballard R, Baird WV, Abbott A, Morgens P, Callahan A, Scorza R, Monet R (1992) Application of RFLP analysis to genetic linkage mapping in peaches. HortScience 27:160–163Google Scholar
  13. Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1992) The extensin from Prunus amygdalus. Plant Physiol 100:1603–1604Google Scholar
  14. Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1995) Molecular characterization of cDNAs corresponding to genes expressed during almond (Prunus amygdalus Batsch) embryo development. Plant Mol Biol 27:205–210Google Scholar
  15. Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57Google Scholar
  16. Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudotestcross: mapping strategy and RAPD markers. Genetics 137:1121–1137PubMedGoogle Scholar
  17. Hauagge R, Rester DE, Asay RA (1987) Isozyme variation among California almond cultivars: I. Inheritance. J Am Soc Hortic Sci 112:687–693Google Scholar
  18. Hemmat M, Weeden NF, Manganaris AG, Lawson DM (1994) Molecular marker linkage map for apple. J Hered 85:4–14PubMedGoogle Scholar
  19. Jackson JE, Clarke GR (1991) Gene flow in an almond orchard. Theor Appl Genet 82:169–173Google Scholar
  20. Kester DE, Asay R (1975) Almonds. In: Janick J, Moore JW (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, La., pp 387–419Google Scholar
  21. Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  22. Lee E, Speir J, Gray J, Brady CJ (1990) Homologies to the tomato endopolygalacturonase gen in the peach genome. Plant Cell Environ 13:513–521Google Scholar
  23. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0, 3rd edn. Whitehead Institute Technical Report, Cambridge, Mass.Google Scholar
  24. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829Google Scholar
  25. Messeguer R, Viruel MA, de Vicente MC, Dettori MT, Quarta R (1994) RFLPs. In: Messeguer R (ed). Methods of molecular marker analysis in Prunus. IRTA Technical Report, Barcelona, pp 26–38Google Scholar
  26. Reiter RS, Williams JGR, Feldman RA, Rafalski JA, Tingey SV, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant imbred lines and random amplified polymorphic DNAs. Proc Natl Acad Sci USA 89:1477–1481Google Scholar
  27. Ritter E, Gebhardt C, Salamini F (1990) Estimation of recombination frequencies and construction of RFLPs map in plants from crosses between heterozygous parents. Genetics 125:645–654PubMedGoogle Scholar
  28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.Google Scholar
  29. Sax K (1933) The origin of the Pomoideae. J Am Soc Hortic Sci 30:147–150Google Scholar
  30. Spiegel-Roy P (1986) Domestication of fruit trees. In: Barigozzi C (ed) The origin and domestication of cultivated plants. Elsevier, Amsterdam Oxford New York Tokyo, pp 201–212Google Scholar
  31. Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744CrossRefGoogle Scholar
  32. Stöcker M, Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1993) A highly conserved α-tubulin sequence from Prunus amygdalus. Plant Mol Biol 22:913–916Google Scholar
  33. Tanksley SD, Miller J, Paterson A, Bernatzky R (1987) Molecular mapping of plant chromosomes. In: Gustafson JP, Apels RA (eds) Chromosome structure in function. Plenum Press, New York, pp 157–173Google Scholar
  34. Tanksley SD, Young ND, Patterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264Google Scholar
  35. Torres AM (1990) Isozyme analysis of tree fruits. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Chapman and Hall, London, pp 192–205Google Scholar
  36. Vowden C, Ridout M (1994) LINREM. A program for genetic linkage analysis. Technical report. Horticultural Research International, East Mailing, EnglandGoogle Scholar
  37. Watkins R (1976) Cherry, plum, peach, apricot and almond. In: Simmonds NW (ed) Evolution of crop plants. Longman, London New York, pp 242–247Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. A. Viruel
    • 1
  • R. Messeguer
    • 1
  • M. C. de Vicente
    • 1
  • J. Garcia-Mas
    • 2
  • P. Puigdomènech
    • 2
  • F. Vargas
    • 3
  • P. Arús
    • 1
  1. 1.Departament de Genètica VegetalInstitut de Recerca i Tecnologia Agroalimentàries (IRTA)Cabrils (Barcelona)Spain
  2. 2.Consejo Superior de Investigaciones Cientifícas (CSIC), Departament de Genètica MolecularCentre d'Investigació i Desenvolupament de BarcelonaBarcelonaSpain
  3. 3.Departament d'Arboricultura MediterràniaInstitut de Recerca i Tecnologia Agroalimentàries (IRTA)Reus (Tarragona)Spain

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