Theoretical and Applied Genetics

, Volume 92, Issue 3–4, pp 448–454 | Cite as

Mapping of the K+/Na+ discrimination locus Kna1 in wheat

  • J. Dubcovsky
  • G. Santa María
  • E. Epstein
  • M.-C. Luo
  • Jan Dvořák


In saline environments, bread wheat, Triticum aestivum L. (genomes AABBDD), accumulates less Na+ and more K+ in expanding and young leaves than durum wheat, T. turgidum L. (genomes AABB). Higher K+/Na+ ratios in leaves of bread wheat correlate with its higher salt tolerance. Chromosome 4D from bread wheat was shown in previous work to play an important role in the control of this trait and was recombined with chromosome 4B in the absence of the Ph1 locus. A population of plants disomic for 4D/4B recombined chromosomes in the genetic background of T. turgidum was developed to investigate the genetic control of K+/Na+ discrimination by chromosome 4D. Evidence was obtained that the trait is controlled by a single locus, designated Kna1, in the long arm of chromosome 4D. In the present work, K+/Na+ discrimination was determined for additional families with 4D/4B chromosomes. The concentrations of Na+ and K+/Na+ ratios in the youngest leaf blades clustered in two nonoverlapping classes, and all recombinant families could be unequivocally assigned to Kna1 and kna1 classes. The Kna1 locus scored this way was mapped on a short region in the 4DL arm and was completely linked to Xwg199, Xabc305, Xbcd.402, Xpsr567, and Xpsr375; it was also mapped as a quantitative trait. The results of the QTL analysis, based on the K+/Na+ ratios in the young leaves of greenhousegrown plants and flag leaves of field-grown plants, agreed with the position of Knal determined as a qualitative trait. Several aspects of gene introgression by manipulation of the Ph1 locus are discussed.

Key words

Wheat Salt tolerance Homoeologous recombination QTL RFLP Genetic marker 


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  1. Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043Google Scholar
  2. Close TJ, Kortt AA, Chandler PM (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13:95–108PubMedGoogle Scholar
  3. Colmer TD, Epstein E, Dvořak J (1995) Differential solute regulation in leaf blades of various ages in salt-sensitive wheat and a salt-tolerant wheat x Lophopyrum elongatum (Host) A. Löve, amphiploid. Plant Physiol 108:1715–1724Google Scholar
  4. Devos KM, Dubcovsky J, Dvořák J, Chinoy CN, Gale MD (1995) Structural evolution of wheat chromosomes 4A, 5A, and 7B and its impact on recombination. Theor Appl Genet 91:282–288Google Scholar
  5. Dubcovsky J, Galvez AF, Dvořák J (1994) Comparison of the genetic organization of the early salt stress response gene system in salt-tolerant Lophopyrum elongatum and salt-sensitive wheat. Theor Appl Genet 87:957–964Google Scholar
  6. Dvořák J, Chen K-C (1984) Distribution of nonstructural variation between wheat cultivars along chromosome arm 6Bp: evidence from the linkage map and physical map of the arm. Genetics 106:325–333Google Scholar
  7. Dvořák J, Gorham J (1992) Methodology of gene transfer by homoeologous recombination into Triticum turgidum: transfer of K+/Na+ discrimination from T. aestivum. Genome 35:639–646Google Scholar
  8. Dvořák J, McGuire PE, Cassidy B (1988) Apparent sources of the A genomes of wheats inferred from the polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30:680–689Google Scholar
  9. Dvořák J, Noaman MM, Goyal S, Gorham J (1994) Enhancement of the salt tolerance of Triticum turgidum L. by the Kna1 locus transferred from the Triticum aestivum L. chromosome 4D by homoeologous recombination. Theor Appl Genet 87:872–877Google Scholar
  10. Dvořák J, Dubcovsky J, Luo M-C, Devos KM, Gale MD (1995) Differentiation between wheat homoeologous chromosomes 4B and 4D. Genome 38:1139–1147Google Scholar
  11. Epstein E (1994) The anomaly of silicon in plant biology. Proc Natl Acad Sci USA 91:11–17Google Scholar
  12. Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat. Genome 34:362–374Google Scholar
  13. Giorgi B, Cuozzo L (1980) Homoeologous pairing in a Ph mutant of tetraploid wheat crossed with rye. Cereal Res Commun 8:485–490Google Scholar
  14. Gorham J, Hardy C, Wyn Jones RG, Joppa LR, Law CN (1987) Chromosome location of a K/Na discrimination character in the D genome of wheat. Theor Appl Genet 74:584–588Google Scholar
  15. Graner A, Jahoor A, Schondelmeier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrman RG (1991 a) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256Google Scholar
  16. Huang Z-Z, Yan X, Jalil A, Norlyn JD, Epstein E (1992) Short-term experiments on ion transport by seedlings and excised roots: technique and validity. Plant Physiol 100:1914–1920Google Scholar
  17. Jena KK, Khush GS, Kodiert G (1992) RFLP analysis of rice (Oryz sauva L.) introgression lines. Theor Appl Genet 84:608–616Google Scholar
  18. Joppa LR, Williams ND (1988) Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat. Genome 30:222–228Google Scholar
  19. Katayama T (1965) Cytogenetic studies of the genus Oryza: 1. Chromosome pairing of the inter-specific hybrid O. saliva x O. officinalis under different temperature conditions. Jpn J Genet 40:307–313Google Scholar
  20. Khursheed B, Rogers JC (1988) Barley alpha-amylase genes. Quantitative comparison of steady-state mRNA levels from individual members of the two different families expressed in aleurone cells. J Biol Chem 263:18953–18960Google Scholar
  21. Kleinhofs A, Kilian A, Saghai MA, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrels M, Heun M, Franckowiak JD, Huffman D, Skadsen R, Steffenson BJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712Google Scholar
  22. Kosambi DD (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  23. Lander ES, Green P, Abrahamson J, Barlow A, Daly M, Lincoln SE, Newburg L (1987) MAPMAKER: an integrated computer package for construction of primary linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  24. Lincoln SE, Daly M, Lander ES (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, 3rd edn, Cambridge, Mass.Google Scholar
  25. Lukaszewski AJ, Curtis CA (1993) Physical distribution of recombination in B-genome chromosomes of tetraploid wheat. Theor Appl Genet 84:121–127Google Scholar
  26. Omielan J, Epstein E, Dvořák J (1991) Salt tolerance and ionic relations of wheat as affected by individual chromosomes of salttolerant Lophopymm elongatum. Genome 34:961–974Google Scholar
  27. Sears ER (1981) Transfer of alien genetic material to wheat. In: Evans LT, Peacock WJ (eds) Wheat science - today and tomorrow. Cambridge University Press, London, pp 75–89Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. Dubcovsky
    • 1
  • G. Santa María
    • 2
  • E. Epstein
    • 2
  • M.-C. Luo
    • 1
  • Jan Dvořák
    • 1
  1. 1.Department of Agronomy and Range ScienceUniversity of CaliforniaDavisUSA
  2. 2.Department of Land, Air and Water ResourcesUniversity of CaliforniaDavisUSA

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