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Theoretical and Applied Genetics

, Volume 93, Issue 8, pp 1282–1290 | Cite as

Length polymorphism and homologies of microsatellites in several Cucurbitaceae species

  • N. Katzir
  • Y. Danin-Poleg
  • G. Tzuri
  • Z. Karchi
  • U. Lavi
  • P. B. Cregan
Article

Abstract

The objectives of this research were to assess (1) the degree of Simple Sequence Repeats (SSR) DNA length polymorphism in melon (Cucumis melo L.) and other species within the Cucurbitaceae family and (2) the possibility of utilizing SSRs flanking primers from single species to other genera or species of Cucurbitaceae. Five melon (CT/GA) n SSRs were isolated from a genomic library. Two cucumber (Cucumis sativus L.) SSRs were detected through a search of DNA sequence databases, one contained a (CT)8 repeat, the other a (AT)13 repeat. The seven SSRs were used to test a diverse sample of Cucurbitaceae, including 8 melon, 11 cucumber, 5 squash, 1 pumpkin, and 3 watermelon genotypes. Five of the seven SSRs detected length polymorphism among the 8 melon genotypes. PCR amplification revealed between three and five length variants (alleles) for each SSR locus, with gene diversity values ranging from 0.53 to 0.75. Codominant segregation of the alleles among F2 progeny was demonstrated for each of the five SSR loci. Four of the seven SSRs detected polymorphism among the 11 cucumber genotypes, with gene diversity values ranging between 0.18 and 0.64. Primers specific to SSRs of C. melo and C. sativus also amplified DNA extracted from genotypes belonging to other genera of the Cucurbitaceae family.

Key words

Simple Sequence Repeats (SSR) Microsatellites DNA markers Cucumis melo L. Cucurbitaceae Melon 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • N. Katzir
    • 1
  • Y. Danin-Poleg
    • 1
  • G. Tzuri
    • 1
  • Z. Karchi
    • 1
  • U. Lavi
    • 2
  • P. B. Cregan
    • 3
  1. 1.Department of Vegetable CropsAgricultural Research Organization, Northern Research CenterHaifaIsrael
  2. 2.Institute of Horticulture, Agricultural Research Organization, The Volcani CenterBet DaganIsrael
  3. 3.USDA-ARS, BARC-WestBeltsvilleUSA

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