Theoretical and Applied Genetics

, Volume 93, Issue 8, pp 1202–1210

PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp.

  • M. Hill
  • H. Witsenboer
  • M. Zabeau
  • P. Vos
  • R. Kesseli
  • R. Michelmore
Article
  • 209 Downloads

Abstract

AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp. Genetic distances based on AFLP data were estimated for 44 morphologically diverse lines of cultivated L. sativa and 13 accessions of the wild species L. serriola, L. saligna, L. virosa, L. perennis, and L. indica. The same genotypes were analyzed as in a previous study that had utilized RFLP markers. The phenetic tree based on AFLP data was consistent with known taxonomic relationships and similar to a tree developed with RFLP data. The genetic distance matrices derived from AFLP and RFLP data were compared using least squares regression analysis and, for the cultivar data, by principal component analysis. There was also a positive linear relationship between distance estimates based on AFLP data and kinship coefficients calculated from pedigree data. AFLPs represent reliable PCR-based markers for studies of genetic relationships at a variety of taxonomic levels.

Key words

AFLP marker Lactuca Lettuce Phylogenetic analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Hill
    • 1
  • H. Witsenboer
    • 2
  • M. Zabeau
    • 3
  • P. Vos
    • 3
  • R. Kesseli
    • 1
  • R. Michelmore
    • 2
  1. 1.Department of BiologyUniversity of MassachusettsBostonUSA
  2. 2.Department of Vegetable CropsUniversity of CaliforniaDavisUSA
  3. 3.Keygene BVAE, WageningenThe Netherlands

Personalised recommendations