Theoretical and Applied Genetics

, Volume 92, Issue 2, pp 170–172 | Cite as

Intergeneric somatic hybrid plantlets between Dianthus barbatus and Gypsophila paniculata obtained by electrofusion

  • M. Nakano
  • Y. Hoshino
  • M. Mii
Article
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Abstract

Hypocotyl-derived protoplasts of Dianthus barbatus that had been pretreated with iodoacetamide were fused electrically with cell suspension culture-derived protoplasts of Gypsophila paniculata that could divide to form callus but could not regenerate shoots under the culture conditions used in this study. Electrofusion-derived calli which produced shoots were selected as putative somatic hybrids, and plantlets were subsequently regenerated from 2 of these selected calli. These plantlets, which in vitro produced flowers precociously, were identified as intergeneric somatic hybrids by nuclear ribosomal DNA analysis. Normal plants have not been established up to the present.

Key words

Dianthus barbatus Electrofusion Gypsophila paniculata Intergeneric somatic hybridization Plantlet regeneration 
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Nakano
    • 1
  • Y. Hoshino
    • 2
  • M. Mii
    • 2
  1. 1.Graduate School of Science and Technology, Niigata UniversityNiigataJapan
  2. 2.Faculty of HorticultureChiba UniversityChibaJapan

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