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Theoretical and Applied Genetics

, Volume 93, Issue 3, pp 421–425 | Cite as

RFLP mapping in barely of a dominant gene conferring resistance to scald (Rhynchosporium secalis)

  • A. Graner
  • A. Tekauz
Article

Abstract

A progeny consisting of 52 anther-derived doubled haploid barley lines from a F1 between the winter cultivars ‘Igri’ (susceptible) and ‘Triton’ (resistant) was tested for resistance to Rhynchosporium secalis. A dominant gene was detected and tagged by a series of cosegregating RFLP markers located in the proximal portion of the long arm of chromosome 3, close to the centromere. One of the cosegregating RFLP markers, cMWG680, was converted into a codominant sequence tagged site marker. Polymerase chain reaction analysis with this marker of a series of accessions carrying known resistance genes provided evidence that scald resistance in cv ‘Triton’ is due to the presence of the Rh gene.

Key words

Distored segregation Doubled haploids Hordeum vulgare STS marker PCR 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • A. Graner
    • 1
  • A. Tekauz
    • 2
  1. 1.Institute for Resistance GeneticsGrünbachGermany
  2. 2.Research Centre, Agriculture and Agri-Food CanadaWinnipegCanada

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