Theoretical and Applied Genetics

, Volume 92, Issue 1, pp 83–86 | Cite as

Association of RFLP markers with loci conferring broad-based resistance to the soybean cyst nematode (Heterodera glycines)

  • R. A. Vierling
  • J. Faghihi
  • V. R. Ferris
  • J. M. Ferris


Soybean cyst nematode (SCN) is a major soybean yield-limiting pest. The present study was conducted to map broad-based SCN resistance loci from the cultivar ‘Hartwig’. Two-hundred F2∶3 lines derived from the cross ‘Williams 82’ x ‘Hartwig’ were screened with a fourth-generation SCN inbred and 56 polymorphic molecular markers. Allele states and phenotypes were analyzed using stepwise regression and the model selection was made at P ⩽ 0.01. Four unlinked RFLP markers (A006, A567, A487, A112) were associated with SCN resistance and the partial coefficient of determinations (R2) were 91%, 1%, 1%, and 1%. We have mapped a new, major SCN resistance locus (A006) and three minor loci (A567, A487, A112). This complete mapping will accelerate the transfer of broad-based resistance without linkage drag and aid in the determination of relationships among various SCN-resistant germplasm sources.

Key words

Resistance genes Soybean cyst nematode Hartwig RFLP mapping PI 437654 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • R. A. Vierling
    • 1
  • J. Faghihi
    • 2
  • V. R. Ferris
    • 2
  • J. M. Ferris
    • 2
  1. 1.Indiana Crop Improvement Association and Department of AgronomyPurdue UniversityWest LafayetteUSA
  2. 2.Department of EntomologyPurdue UniversityWest LafayetteUSA

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