Theoretical and Applied Genetics

, Volume 90, Issue 7–8, pp 907–913

A chromosome 5-specific repetitive DNA sequence in rice (Oryza sativa L)

  • Z. X. Wang
  • N. Kurata
  • S. Saji
  • Y. Katayose
  • Y. Minobe
Article

Abstract

Repetitive DNA sequences in the rice genome comprise more than half of the nuclear DNA. The isolation and characterization of these repetitive DNA sequences should lead to a better understanding of rice chromosome structure and genome organization. We report here the characterization and chromosome localization of a chromosome 5-specific repetitive DNA sequence. This repetitive DNA sequence was estimated to have at least 900 copies. DNA sequence analysis of three genomic clones which contain the repeat unit indicated that the DNA sequences have two sub-repeat units of 37 bp and 19 bp, connected by 30-to 90-bp short sequences with high similarity. RFLP mapping and physical mapping by fluorescence in situ hybridization (FISH) indicated that almost all copies of the repetitive DNA sequence are located in the centromeric heterochromatic region of the long arm of chromosome 5. The strategy for cloning such repetitive DNA sequences and their uses in rice genome research are discussed.

Key words

Rice Repetitive DNA sequence RFLP mapping Fluorescence in situ hybridization(FISH) Physical map 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Z. X. Wang
    • 1
  • N. Kurata
    • 1
  • S. Saji
    • 1
  • Y. Katayose
    • 1
  • Y. Minobe
    • 1
  1. 1.Rice Genome Research ProgramNational Institute of Agrobiological Resources /STAFF (Society for Techno-innovation of Agriculture, Forestry and Fisheries) InstituteIbarakiJapan

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