Theoretical and Applied Genetics

, Volume 86, Issue 6, pp 705–712 | Cite as

A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

  • A. Kleinhofs
  • A. Kilian
  • M. A. Saghai Maroof
  • R. M. Biyashev
  • P. Hayes
  • F. Q. Chen
  • N. Lapitan
  • A. Fenwick
  • T. K. Blake
  • V. Kanazin
  • E. Ananiev
  • L. Dahleen
  • D. Kudrna
  • J. Bollinger
  • S. J. Knapp
  • B. Liu
  • M. Sorrells
  • M. Heun
  • J. D. Franckowiak
  • D. Hoffman
  • R. Skadsen
  • B. J. Steffenson
Article

Abstract

A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

Key words

RFLP Mapping Barley Genome Centromeres 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Kleinhofs
    • 1
    • 2
  • A. Kilian
    • 1
  • M. A. Saghai Maroof
    • 3
  • R. M. Biyashev
    • 3
  • P. Hayes
    • 4
  • F. Q. Chen
    • 4
  • N. Lapitan
    • 5
  • A. Fenwick
    • 5
  • T. K. Blake
    • 6
  • V. Kanazin
    • 6
  • E. Ananiev
    • 6
  • L. Dahleen
    • 7
  • D. Kudrna
    • 1
  • J. Bollinger
    • 1
  • S. J. Knapp
    • 4
  • B. Liu
    • 4
  • M. Sorrells
    • 8
  • M. Heun
    • 8
  • J. D. Franckowiak
    • 9
  • D. Hoffman
    • 10
  • R. Skadsen
    • 11
  • B. J. Steffenson
    • 12
  1. 1.Department of Crop and Soil SciencesWashington State UniversityPullmanUSA
  2. 2.Department of Genetics and Cell BiologyWashington State UniversityPullmanUSA
  3. 3.Department of Crop and Soil Environmental SciencesVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  4. 4.Crop Sciences DepartmentOregon State UniversityCorvalisUSA
  5. 5.Department of AgronomyColorado State UniversityFort CollinsUSA
  6. 6.Department of Plant and Soil SciencesMontana State UniversityBozemanUSA
  7. 7.USDA-ARS, Northern Crop Science LaboratoryFargoUSA
  8. 8.Department of Plant BreedingCornell UniversityIthacaUSA
  9. 9.Department of Crop and Weed SciencesNorth Dakota State UniversityFargoUSA
  10. 10.USDA-ARS, National Small Grains Germplasm Research FacilityAberdeenUSA
  11. 11.Cereal Crops Research UnitMadisonUSA
  12. 12.Department of Plant PathologyNorth Dakota State UniversityFargoUSA
  13. 13.Department of GeneticsSilesian UniversityKatowicePoland
  14. 14.Department of Plant PathologyNorth Carolina State UniversityRaleighUSA

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