5-HT-like immunoreactivity in the central nervous system of the crayfish, Pacifastacus leniusculus
An immunocytochemical technique with the use of three different antibodies raised against serotonin was applied to localize the immunoreactive neurons in the central nervous system of the crayfish, Pacifastacus leniusculus. Immunoreactive neurons were found in three optic ganglia (medulla externa, interna and terminalis). They appeared in three layers of the medulla externa and interna. The medulla terminalis displayed three prominent groups of immunoreactive perikarya and mainly marginal immunoreactive fibres. Immunoreactive areas of the brain comprised the protocerebral bridge, central body, paracentral lobes and two loci in the anterior portion of the protocerebrum, i.e., the terminal areas for immunoreactive fibres from the optic centres. The olfactory lobes showed a specific immunoreactive pattern. In addition, diffusely and sparsely distributed immunoreactive fibres were found throughout the brain. The immunoreactive neurons are largely localized in the same areas of the central nervous system as the catecholaminergic neurons although some distinct differences occur.
Key wordsSerotonin Immunoreactive neurons Central nervous system Crayfish
Unable to display preview. Download preview PDF.
- Aramant R, Elofsson R (1976) Monoaminergic neurons in the nervous system of crustaceans. Cell Tissue Res 170:231–251Google Scholar
- Björklund A, Falck B, Owman C (1972) Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines. In: Methods of investigative and diagnostic endocrinology (ed Berson SA), Vol 1 The thyroid and biogenic amines (eds Rall JE, Kopin IJ) North-Holland, Amsterdam, pp 318–368Google Scholar
- Elofsson R, Klemm N (1972) Monoamine-containing neurons in the optic ganglia of crustaceans and insects. Z Zellforsch mikrosk Anat 133:475–499Google Scholar
- Elofsson R, Kauri T, Nielsen S-O, Strömberg J-O (1966) Localization of monoaminergic neurons in the central nervous system of Astacus astacus Linné (Crustacea). Z Zellfosch mikrosk Anat 74:464–473Google Scholar
- Elofsson R, Nässel DR, Myhrberg H (1977) A catecholaminergic neuron connecting the first two optic neuropiles (lamina ganglionaris and medulla externa) of the crayfish Pacifastacus leniusculus. Cell Tissue Res 182:287–297Google Scholar
- Elofsson R, Laxmyr L, Rosengren E, Hansson C (1982) Identification and quantitative measurements of biogenic amines and dopa in the central nervous system and haemolymph of the crayfish Pacifastacus leniusculus (Crustacea). Comp Biochem Physiol 71C: 195–201Google Scholar
- Falck B (1962) Observations on the possibilities of the cellular localization of monoamines by a fluorescence method. Acta Physiol Scand 56 Suppl 197:1–25Google Scholar
- Myhrberg H, Elofsson R, Aramant R, Klemm N, Laxmyr L (1979) Selective uptake of exogenous catecholamines into nerve fibres in crustaceans. A fluorescence histochemical investigation. Comp Biochem Physiol 62C:141–150Google Scholar
- Steinbusch HWM, Verhofstad AAJ, Joosten HWJ (1978) Localization of serotonin in the central nervous system by immunohistochemistry: description of a specific and sensitive technique and some applications. Neuroscience 3:811–819Google Scholar
- Sternberger LA (1974) Immunocytochemistry. Prentice-Hall Inc, New JerseyGoogle Scholar
- Strausfeld NJ, Nässel DR (1980) Neuroarchitecture of brain regions that subserve the compound eyes of Crustacea and Insects. In: (ed Autrum H) Handbook of sensory physiology, Vol 7/6B Comparative physiology and evolution of vision in invertebrates. Springer Verlag, Berlin Heidelberg New York, pp 1–132Google Scholar