Cell and Tissue Research

, Volume 232, Issue 1, pp 201–219 | Cite as

Ultrastructural studies on the thymus gland after the administration of phenylhydrazine to bank voles (Clethrionomys glareolus)

  • Marion D. Kendall
  • N. M. Blackett
Article
Accepted:

Summary

The thymus glands of wild and laboratory reared bank voles (Clethrionomys glareolus) were examined for ultrastructural changes before and after the administration of low (20 mg/kg) and high (60 mg/ kg) doses of phenylhydrazine (PhZ) given intraperitoneally on days 0, 1 and 3 of a dosing regime.

Erythrocytes developed in focal aggregations in the subcapsular and outer cortical zones. There was a marked depletion of the subcapsular cortex following drug treatment that was reversed with time. A large number of granulocytes were visible in the cortex after PhZ, and mast cells developed in situ.

Key words

Thymus Phenylhydrazine Anaemia Thymic erythropoiesis Mast cells 
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References

  1. Abe K, Ito T (1970) Fine structure of small lymphocytes in the thymus of the mouse: Qualitative and quantitative analysis by electron microscopy. Z Zellforsch mikrosk Anat 110:321–335Google Scholar
  2. Abe K, Ito T (1972) A new stereological method for determination of the size of spherical objects in electron microscopy: Its application to small lymphocytes of the mouse thymus. Arch Histol Jpn 34:203–214Google Scholar
  3. Abe K, Sasaki K, Ito T (1973) Comparative ultrastructure and cytometric analysis of small lymphocytes in haemopoietic organs of neonatal mice. J Anat 115:393–406Google Scholar
  4. Bacchus S, Kendall MD (1975) Histological changes associated with enlargement and regression of the thymus glands of the red-billed quelea Quelea quelea L. (Ploceidae: weaverbirds). Phil Trans R Soc Lond (Biol) 273:65–78Google Scholar
  5. Brecher G, Stohlman F (1961) Reticulocyte size and erythropoietic stimulation. Proc Soc Exp Biol Med 107:887–891Google Scholar
  6. Burnet FM (1965) Mast cells in the thymus of NZB mice. J Pathol Bact 89:271–284Google Scholar
  7. Cline MJ, Golde DW (1979) Cellular interactions in haematopoiesis. Nature (London) 277:177–181Google Scholar
  8. Dacie JV, Lewis SM (1970) Practical haematology. 4th Edn. Churchill: LondonGoogle Scholar
  9. Dappen GE, Crouse DA, Anderson RW, Jordan RK, Robinson JH, Sharp JG (1981) Morphological assessment of immunologically relevant cells in the thymus. Proc 7th Int Conf Lymphatic Tissues and Germinal Centres in Immune Reactions. Plenum Press: New YorkGoogle Scholar
  10. Ewijk W, van Verzijden JHM, Kwast ThH van der, Luijex-Meijer SWM (1974) Reconstitution of the thymus dependant area in the spleen of lethally irradiated mice. A light and electron microscopical study of the T-cell microenvironment. Cell Tissue Res 149:43–60Google Scholar
  11. Goldstein G (1966) Mast cells in the human thymus. Aust J Exp Biol Med Sci 44:593–596Google Scholar
  12. Grasso JA (1973) Erythropoiesis in the newt Triturus cristatus Laur. 1. Identification of the “erythroid precursor cell”. J Cell Sci 12:463–489Google Scholar
  13. Hanna IRA, Tarbutt RG, Lamerton LF (1969) Shortening of the cell-cycle time of erythroid precursors in response to anaemia. Br J Haematol 16:381–387Google Scholar
  14. Harris PF, Kugler JH (1971) Unusual mononuclear cells in guinea pig peripheral blood during anaemia. J Anat 108:1–12Google Scholar
  15. Hodgson GS, Bradley TR, Telfer PA (1972) Haemopietic stem cells in experimental haemolytic anaemic. Cell Tissue Kinet 5:283–288Google Scholar
  16. Höhn EO (1947) Seasonal cyclical changes in the thymus of the mallard. J Exp Biol 24:184–191Google Scholar
  17. Hoshino T (1963) Electron microscope studies of the epithelial reticular cells of the mouse thymus. Z Zellforsch mikrosk Anat 59:513–529Google Scholar
  18. Hoshino T, Kukita A, Sato S (1970) Cells containing Birbeck granules (Langerhans cell granules) in the human thymus. J Electron Micros 19:271–276Google Scholar
  19. Ishizaka T, Okudaira H, Mauser LE, Ishizaka K (1976) Development of rat mast cells in vitro. I. Differentiation of mast cells from thymus cells. J Immunol 116:747–754Google Scholar
  20. Jarplid B (1974) Dark reticular cells in the thymus of mice. Acta Radiol (Ther) (Stockh) 13:319–328Google Scholar
  21. Jordan RK, Robinson JH (1981) T-lymphocyte differentiation. In: Kendall MD (ed) The thymus. Anat Soc Symp 1:151–177 Academic Press, LondonGoogle Scholar
  22. Kendall MD (1975) Sizes and numbers of nuclei in the cortex of thymus glands of red-billed weavers Quelea quelea. Cell Tissue Res 164:233–249Google Scholar
  23. Kendall MD (1980) The occurrence of erythropoiesis in the thymus glands of the bank vole Clethrionomys glareolus. Cell Tissue Res 212:307–314Google Scholar
  24. Kendall MD (1981) The cells of the thymus. In: Kendall MD (ed) The thymus. Anat Soc Symp 1:63–83 Academic Press, LondonGoogle Scholar
  25. Kendall MD, Frazier JA (1979) Ultrastructural studies on erythropoiesis in avian thymus glands. I. Description of cell types. Cell Tissue Res 199:37–61Google Scholar
  26. Kendall MD, Twigg GI (1981) The weight of the thymus gland in a population of wild bank voles, Clethrionomys glareolus, from Wicken Fen, Cambridgeshire. J Zool 194:323–339Google Scholar
  27. Mandel T (1968) Ultrastructure of epithelial cells in the medulla of the guinea-pig thymus. Aust J Exp Biol Med Sci 46:735–767Google Scholar
  28. Micklem HS, Anderson N, Ure J, Jones HP (1976) Long-term immunoglobulin G production by transplanted thymus cells. Eur J Immunol 6:425–429Google Scholar
  29. Quesenberry P, Levitt L (1979) Haemopoietic stem cells. New Engl J Med 301:755–760; 819–823; 868–872Google Scholar
  30. Rappay G, Bukulya B, Báscy E (1971) Fine structure, distribution and function of the rat thymus reticular cells in the perinatal life. Acta Biol Acad Sci Hung 22:187–196Google Scholar
  31. Rencricca NJ, Rizzoli V, Howard D, Duffy P, Stohlman F Jr (1970) Stem cell migration and proliferation during severe anaemia. Blood 36:764–771Google Scholar
  32. Riches AC, Cork MJ, Ali AM, Cullan GM, Crouse DA, Anderson RW, Sharp JG (1981) Humoral factors regulating haemopoietic stem cell proliferation. In: Kendall MD (ed) The thymus gland. Anat Soc Symp 1:203–207 Academic Press, LondonGoogle Scholar
  33. Sharp JG, Crouse DA (1981) The role of the thymus in haematopoiesis. In: Baum J, Ledney GD, Bekkum DW van (eds) Experimental haematology today 1980. Karger, BaselGoogle Scholar
  34. Singh J (1981) The ultrastructure of epithelial reticular cells. In: Kendall MD (ed) The thymus. Anat Soc Symp 1:133–150Google Scholar
  35. Stohlman F Jr, Beland A, Howard D (1963) Mechanism of macrocytic response to erythropoietin. J Clin Invest 42:984–985PubMedGoogle Scholar
  36. Tarbutt RG (1969) Cell population kinetics of the erythroid system in the rat: the response to protracted anaemia and to continuous y-irradiation. Br J Haematol 16:9–24Google Scholar
  37. Thomas DB, Sharp JG, Briscoe V (1977) The morphological characterization of the haematopoietic stem cell (CFU-S). J Anat 124:21–30Google Scholar
  38. Thomas N, Maclean N (1974) The blood as an erythropoietic organ in anaemic Xenopus. Experientia (Basel) 30:1083–1085Google Scholar
  39. Veerman AJP (1974) On the interdigitating cells in the thymus-dependent area of the rat spleen: a relation between the mononuclear phagocyte system and T-lymphocytes. Cell Tissue Res 148:247–257Google Scholar
  40. Ward P, Kendall MD (1975) Morphological changes in the thymus of young and adult redbilled queleas Quelea quelea (Aves). Philos Trans R Soc Lond (Biol) 273:55–64Google Scholar
  41. Williams AF (1972) The nature of immature avian erythrocytes in severe anaemia induced by phenylhydrazine. J Cell Sci 11:771–776Google Scholar
  42. Wright EG, Lord BI (1977) Regulation of CFU-S proliferation by locally produced endogenous factors. Biomedicine 27:215–218Google Scholar
  43. Yoffey JM (1981) Stem cells and the thymus with special reference to cellular migration streams. In: Kendall MD (ed) The thymus. Anat Soc Symp 1:185–202. Academic Press, LondonGoogle Scholar

Copyright information

© Springer-Verlag GmbH & Co. KG 1983

Authors and Affiliations

  • Marion D. Kendall
    • 1
  • N. M. Blackett
    • 2
  1. 1.Department of AnatomySt. Thomas's Hospital Medical SchoolLondonUK
  2. 2.Institute of Cancer Research, Chester Beatty Research InstituteLondonUK

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