Theoretical and Applied Genetics

, Volume 90, Issue 2, pp 182–188 | Cite as

Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in Indica/Japonica hybrids of rice (O. sativa L.)

  • S. Yanagihara
  • S. R. McCouch
  • K. Ishikawa
  • Y. Ogi
  • K. Maruyama
  • H. Ikehashi
Article

Abstract

RFLP analysis was conducted on a population derived from a three-way cross to determine the location of the hybrid sterility locus, S-5, in relation to mapped molecular markers and to identify markers that would be useful for selection in breeding. S-5 is of interest to rice breeders because it is associated with spikelet sterility of F1 hybrids in Indica/Japonica crosses. Identification of an S-5 allele which confers fertility in Indica/Japonica hybrids when introgressed into either the Indica or the Japonica parent has been reported. Varieties carrying this S-5n allele are known as “wide compatibility varieties (WCV)”. Our data suggests that RFLP marker RG213 on chromosome 6 is closely linked to the S-5 locus and can be efficiently used to identify wide compatibility (WC) lines. RG213 is a single-copy genomic clone that detects three bands of different molecular weights in DNA from Japonica (‘Akihikari’) and Indica (‘IR36’) varieties and WC line (‘Nekken 2’). We demonstrate that the three alleles detected by this marker could be used to trace the inheritance of the “wide compatible” phenotype in breeders' material.

Key words

Wide compatibility Oryza sativa RFLP markers Hybrid sterility 

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References

  1. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparation: Version II. Plant Mol Biol Rep 1:19–21Google Scholar
  2. Ikehashi H (1991) Genetics of hybrid sterility in wide hybridization in rice (O. sativa L.). In: Bajaj YPS (ed) Rice. (Biotechnology in agriculture and forestry, vol 14). Springer, Berlin Heidelberg New York, pp (113–127)Google Scholar
  3. Ikehashi H, Araki H (1984) Varietal screening for compatibility types revealed in F1 fertility of distant crosses in rice. Jpn J Breed 34:304–312Google Scholar
  4. Ikehashi H, Araki H (1986) Genetics of F1, sterility in remote crosses of rice. In: Rice genetics. IRRI, Manila, Philippines, pp 119–130Google Scholar
  5. Ikehashi H, Araki H (1988) Multiple alleles controlling F1, sterility in remote crosses of rice. Jpn J Breed 38:283–291Google Scholar
  6. Ikehashi H, Araki H, Yanagihara S (1991) Screening and analysis of wide compatibility loci in wide cross of rice. In: Rice genetics. IRRI, Manila, Philippines, pp 33–43Google Scholar
  7. Ishikawa R, Morishima H, Mori K, Kinoshita T (1989) Chromosomal analysis of isozyme loci and the allelic expression at cellular level in rice. J Fac Agric Hokkaido Univ 1:85–98Google Scholar
  8. Kato S (1930) On the affinity of cultivated varieties of rice plants, Oryza sativa L. J Dep Agric Kyushu Imp Univ 2:241–276Google Scholar
  9. Kinoshita T (1993) Report of the committee on gene symbolization, nomenclature and linkage groups. RGN 10:7–39Google Scholar
  10. Kitamura E (1962) Genetic studies on sterility observed in hybrids between distantly related varieties of rice, Oryza sativa. Rep Chugoku Exp Sta A 8:141–205Google Scholar
  11. Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural population. Genomics 1:174–181PubMedGoogle Scholar
  12. Leidl B, Anderson NO (1993) Reproductive barriers: identification, uses and circumvention. Plant Breed Rev 11:11–154Google Scholar
  13. Lincoln S, Daily M, Lander E (1992) Mapping genes controlling quantitative traits with MAPMAKER/QTL 11. Whitehead Institute Technical Rep, 2nd ednGoogle Scholar
  14. Liu A, Li H, Zhang Q, Jiang X, Shi S, Yang G (1992) Mapping a wide compatibility gene of rice in relation to RFLP markers. J Huazhong Agric Univ 11:213–219Google Scholar
  15. Mackill M, Salam A, Wang ZY, Tanksley SD (1993) A major photoperiod sensitivity gene tagged with RFLP and isozyme markers in rice. Theor Appl Genet 85:536–540Google Scholar
  16. Manly KF (1993) A Macintosh program for storage and analysis of experimental genetic mapping data. Mammalian Genome 4:303–313Google Scholar
  17. Morinaga T (1954) Classification of rice varieties on the basis of affinity. In: Rep 5th Meet Int Rice Comm Working Party Rice Breed. MAF, Japan, 1–14Google Scholar
  18. Nakagahra M (1977) Genetic analysis for esterase isozymes in rice cultivars. Jpn J Breed 27:141–148Google Scholar
  19. Oka HI (1953) The mechanisms of sterility in the intervarietal hybrids. Phylogenetic differentiation of cultivated rice. XII. Jpn J Breed 2:217–224Google Scholar
  20. Oka HI (1974) Analysis of genes controlling F1 sterility in rice by the use of isogenic lines. Genetics 77:521–534Google Scholar
  21. Panaud O, Magapantay G, McCouch SR (1993) A protocol for nonradioactive DNA labelling and detection in the analysis of rice and tomato using single-copy probes. Plant Mol Biol Rep 11:54–59Google Scholar
  22. Tai TH, Tanksley SD (1991) A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol Biol Rep 8:297–303Google Scholar
  23. Tanksley S, Causse M, Fulton T, Ahn N, Wang Z, Wu K, Xiao J, Ronald P, Yu Z, Second G, McCouch SR (1992) A high density molecular map of the rice genome. Rice Genet Newsl 9:111–115Google Scholar
  24. Terao H, Mizushima U (1939) Some consideration on the classification of Oryza sativa L. into subspecies, so-called Japonica and Indica. Jpn J Bot 10:213–258Google Scholar
  25. Wan J, Yanagihara S, Kato H, Ikehashi H (1993a) Multiple alleles at a new locus causing hybrid sterility between a Korean indica variety and a Javanica variety in rice (Oryza sativa L.). Jpn J Breed 43:507–516Google Scholar
  26. Wan J, Yanagihara S, Kato H, Ikehashi H (1993b) A new locus for hybrid sterility in remote cross of cultivated rice (O. sativa L.) III S-9(t). Jpn J Breed 43[Suppl 1]:148Google Scholar
  27. Yanagihara S, Ikehashi H, Araki H, Maruyama K (1992a) Difference in recombination frequency between Chromogen (C) and Waxy (wx) genes in Japonica and Indica rice (Oryza sativa L.) by using Japonica lines with a wide compatibility (S-5 n). Jpn J Breed 42:15–21Google Scholar
  28. Yanagihara S, Kato H, Ikehashi H (1992b) A new locus for multiple alleles causing hybrid sterility between an aus variety and javanica varieties in rice (Oryza sativa L.). Jpn J Breed 42:793–810Google Scholar
  29. Yu ZM, Mackill DJ, Borman JM, Tanksley SD (1992) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476Google Scholar
  30. Zheng K, Shen P, Quian H, Wang J (1992) Tagging genes for wide compatibility in rice via linkage to RFLP markers. Chinese Rice Sci 6:145–150Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. Yanagihara
    • 1
  • S. R. McCouch
    • 1
  • K. Ishikawa
    • 2
  • Y. Ogi
    • 3
  • K. Maruyama
    • 4
  • H. Ikehashi
    • 5
  1. 1.Department of Plant Breeding and BiometryCornell UniversityIthacaUSA
  2. 2.Faculty of HorticultureChiba UniversityChibaJapan
  3. 3.Kyoto prefectural tea industry Research InstituteKyotoJapan
  4. 4.Agriculture, Forestry and Fisheries's Research Council, SecretariatTokyoJapan
  5. 5.Faculty of AgricultureKyoto UniversityKyotoJapan

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