Theoretical and Applied Genetics

, Volume 92, Issue 7, pp 865–872 | Cite as

Inheritance and linkage relationships of isozyme and morphological loci in cucumber (Cucumis sativus L.)

  • V. Meglic
  • J. E. Staub
Article

Abstract

Twenty-one polymorphic and 17 monomorphic cucumber (Cucumis sativus L.) isozyme loci were identified in 15 enzyme systems. Seven of the polymorphic loci (Ak-2, Ak-3, Fdp-1, Fdp-2, Mpi-1, Pep-gl, and Skdh) had not been described previously. Segregation in F2 and BC families for isozyme and morphological loci demonstrated agreement with the expected 1∶2∶1 and 1∶1 segregation ratio (P<0.01). Nine morphological markers were found to be linked to isozyme loci and were integrated to form a map containing four linkage groups spanning 584 cM with a mean linkage distance of approximately 19 cM. Linkage groups (A to D) contain the following loci in genetic order: Apsl, Pep-la, B, Per, dm, Pgm, Mpi-1, Idh, Ar, Fdp-1, Ak-2, Pgd-1, Mpi-2 and gl; Blh, Mdh-2, Pep-gl, Pgd-2, Fdp-2, Ccu, Mdh-3, Ak-3, ll, de, F and Mdh-1, and Gr; Ccor, Gpi, and Skdh; DTu and ss. This study detected four new linkages between morphological markers (dm-psl, de-ll, ll-F, and de-F) and confirmed previously reported linkages, dm-Ar and Tu-ss. The isozyme/morphological map constructed in this study led to a more comprehensive understanding of the genetic relationships between several economically important traits.

Key words

Isozymes Genetic markers Disease resistance Genetic map 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abul-Hayja Z (1975) Multiple disease screening and genetics of resistance in cucumber. PhD thesis. University of Wisconsin, Madison, Wis.Google Scholar
  2. Abul-Hayja Z, Williams PH, Peterson CE (1975) Independence of scab and bacterial wilt resistance and ten seedling markers in cucumber. HortScience 10:423–424Google Scholar
  3. Allendorf FW, Mitchell N, Ryman N, Stahl G (1977) Isozyme loci in brown trout (Salmon trutta L.): detection and interpretation from population data. Hereditas 86:179–190Google Scholar
  4. Arús P (1983) Genetic purity of commercial seedlots. In: Tanksley SD, Orton TJ (eds.) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 415–424Google Scholar
  5. Bailey DC (1983) Isozymic variation and the plant breeder's rights. In: SD Tanksley, Orton TJ (eds). Isozymes in plant breeding and genetics. Elsevier, Amsterdam, pp 425–440Google Scholar
  6. Beckman JS, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping, and costs. Theor Appl Genet 67:35–43Google Scholar
  7. Birchler JA (1983) Allozymes in gene dosage studies. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 85–100Google Scholar
  8. Brewer GB (1970) An introduction to isozyme techniques. Academic Press, New YorkGoogle Scholar
  9. Brown AHD, Weir BS (1983) Measuring genetic variability in plant populations. In Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 219–239Google Scholar
  10. Clayton JW, Tretiak DN (1972) Amine-citrate buffers for pH control in starch gel electrophoresis. J Fish Res Board Can 29:1169–1172Google Scholar
  11. Crawford DJ (1983) Phylogenetic and systematic inferences from electrophoretic studies. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier. Amsterdam, pp 257–288Google Scholar
  12. Fanourakis NE, Simon PW (1987a) Analysis of genetic linkage in the cucumber. J Hered 78:238–242Google Scholar
  13. Frederick LR, Staub JE (1989) Combining ability analysis of fruit yield and quality in near-homozygous lines derived from cucumber. J Am Soc Hortic Sci 114:332–338Google Scholar
  14. Frei OM, Stuber CW, Goodman MM (1986) Yield manipulation from selection on allozyme genotypes in a composite of elite corn lines. Crop Sci 26:917–921Google Scholar
  15. Guse RA, Coors JG, Drolsom PN, Tracy WF (1988) Isozyme marker loci associated with cold tolerance and maturity in maize. Theor Appl Genet 76:398–404Google Scholar
  16. Kennard WC, Poetter K, Dijkhuizen A, Meglic V, Staub JE, Havey MJ (1994) Linkages among RFLP, RAPD, isozyme, diseaseresistance, and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet 89:42–48Google Scholar
  17. Knerr LD, Staub JE (1992) Inheritance and linkage relationships of isozyme loci in cucumber (Cucumis sativus L.). Theor Appl Genet 84:217–224Google Scholar
  18. Knerr LD, Staub JE, Holder DJ, May BP (1989) Genetic diversity in Cucumis sativus L. assessed by variation at 18 allozyme coding loci. Theor Appl Genet 78: 119–128Google Scholar
  19. Knerr LD, Meglic V, Syaub JE (1994) A fourth malate dehydrogenase (MDH) locus in cucumber. HortScience 30:118–119Google Scholar
  20. Lander ES, Botstein D (1986) Mapping complex genetic traits in humans: new method using a complex RFLP linkage map. Cold Spring Harbor Symp Quant Biol 51:49–62Google Scholar
  21. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  22. Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116: 331–337Google Scholar
  23. Lassner MW, Orton TJ (1983) Detection of somatic variation. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 209–218Google Scholar
  24. Lo Schiavo F, Giuliano G, Terzi M (1983) Identifying natural and parasexual hybrids. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 305–312Google Scholar
  25. Meglic V (1994) Inheritance and linkage relationship between biochemical and morphological loci in cucumber (Cucumis sativus L.). PhD thesis, University of Wisconsin-Madison, Wis.Google Scholar
  26. Nielsen G (1985) The use of isozymes as probes to identify and label plant varieties and cultivars. In: Rattazzi MC, Scandalios JG, Whitt GS (eds) Isozymes; current topics in biological and medical research, Vol 12. Alan R Liss, New York, pp 1–32Google Scholar
  27. O'Brien SJ (1990) Genetic maps: locus maps of complex genomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.Google Scholar
  28. Pierce LK, Wehner TC (1990) Review of genes and linkage groups in cucumber. HortScience 25:605–615PubMedGoogle Scholar
  29. Pierce LC, Brewbaker JL (1973) Applications of isozyme analysis in horticultural science. HortScience 8:17–22Google Scholar
  30. Ramachandran C, Seshadri VS (1986) Cytological analysis of the genome of cucumber (Cucumis sativus L.) and moskmelon (Cucumis melo L). Z Pflanzenzüchtung 96:25–38Google Scholar
  31. Richmond RC (1972) Enzyme variability in the Drosophila williston group. 3. Amounts of variability in the superspecies D. paulistorum. Genetics 70:87–112Google Scholar
  32. Rick CM, Fobes JF (1974) Association of an allozyme with nematode resistance. Rep Tomato Genet Coop 24:25Google Scholar
  33. Ridgway GJ, Sherburne SW, Lewis RD (1970) Polymorphism in the esterases of Atlantic herring. Trans Am Fish Soc 99:147–151Google Scholar
  34. Ritland K (1983) Estimation of mating systems. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 289–302Google Scholar
  35. Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyseus. I. Variation in the old-field mouse (Peromyseus polionotus). In: Studies in genetics. University of Texas Publication, AustinGoogle Scholar
  36. Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes: a compilation of recipes. Biochem Genet 4:297–320PubMedGoogle Scholar
  37. Smith SS, Luk S, Sobral B, Muhawish S, Peleman J, Zabeau JM (1994) Association among inbred lines of maize using RFLP and DNA amplification technologies (AFLP and AP-PCR), and correlation with pedigree, F1 yield and heterosis. Maize Genet Newsl 68:45Google Scholar
  38. Staub JE, Meglic V (1993) Molecular genetic markers and their relevance for cultivar discrimination: a case study in cucumber. HortTechnology 3: 291–299Google Scholar
  39. Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics: 823–839Google Scholar
  40. Tanksley SD, Rick CM, Vallejos CE (1984) Tight linkage between a nuclear male-sterile locus and an enzyme marker in tomato. Theor Appl Genet 68:109–113Google Scholar
  41. Vallejos CE, Tanksley SD (1983) Segregation of isozyme markers and cold tolerance in an interspecific backcross of tomato. Theor Appl Genet 66:241–247Google Scholar
  42. Weeden NF, Wendel JF (1989) Genetics of plant isozymes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides Press, Portland, Ore., pp 46–72Google Scholar
  43. Whelan EDP, Williams PH, Abul-Hayja A (1975) The inheritance of two induced cotyledon mutants of cucumber. HortScience 10:267–269Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • V. Meglic
    • 1
  • J. E. Staub
    • 1
  1. 1.USDA-ARS, Vegetable Crops Unit, Department of HorticultureUniversity of WisconsinMadisonUSA

Personalised recommendations