Theoretical and Applied Genetics

, Volume 92, Issue 7, pp 817–826 | Cite as

Comparative mapping in F2∶3 and F6∶7 generations of quantitative trait loci for grain yield and yield components in maize

  • D. F. Austin
  • M. Lee
Article

Abstract

This study was conducted to compare maize quantitative trait loci (QTL) detection for grain yield and yield components in F2∶3 and F6∶7 recombinant inbred (RI) lines from the same population. One hundred and eighty-six F6∶7 RIs from a Mo17×H99 population were grown in a replicated field experiment and analyzed at 101 loci detected by restriction fragment length polymorphisms (RFLPs). Single-factor analysis of variance was conducted for each locus-trait combination to identify QTL. For grain yield, 6 QTL were detected accounting for 22% of the phenotypic variation. A total of 63 QTL were identified for the seven grain yield components with alleles from both parents contributing to increased trait values. Several genetic regions were associated with more than one trait, indicating possible linked and/or pleiotropic effects. In a comparison with 150 F2∶3 lines from the same population, the same genetic regions and parental effects were detected across generations despite being evaluated under diverse environmental conditions. Some of the QTL detected in the F2∶3 seem to be dissected into multiple, linked QTL in the F6∶7 generation, indicating better genetic resolution for QTL detection with RIs. Also, genetic effects at QTL are smaller in the F6∶7 generation for all traits.

Key words

Zea mays RFLPs Plant breeding Genetics Recombination 

Abbreviations

RFLPs

Restriction fragment length polymorphisms

QTL

quantitative trait loci

RIs

recombinant inbreds

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • D. F. Austin
    • 1
  • M. Lee
    • 1
  1. 1.Department of AgronomyIowa State UniversityAmesUSA

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