Theoretical and Applied Genetics

, Volume 92, Issue 7, pp 817–826 | Cite as

Comparative mapping in F2∶3 and F6∶7 generations of quantitative trait loci for grain yield and yield components in maize

  • D. F. Austin
  • M. Lee


This study was conducted to compare maize quantitative trait loci (QTL) detection for grain yield and yield components in F2∶3 and F6∶7 recombinant inbred (RI) lines from the same population. One hundred and eighty-six F6∶7 RIs from a Mo17×H99 population were grown in a replicated field experiment and analyzed at 101 loci detected by restriction fragment length polymorphisms (RFLPs). Single-factor analysis of variance was conducted for each locus-trait combination to identify QTL. For grain yield, 6 QTL were detected accounting for 22% of the phenotypic variation. A total of 63 QTL were identified for the seven grain yield components with alleles from both parents contributing to increased trait values. Several genetic regions were associated with more than one trait, indicating possible linked and/or pleiotropic effects. In a comparison with 150 F2∶3 lines from the same population, the same genetic regions and parental effects were detected across generations despite being evaluated under diverse environmental conditions. Some of the QTL detected in the F2∶3 seem to be dissected into multiple, linked QTL in the F6∶7 generation, indicating better genetic resolution for QTL detection with RIs. Also, genetic effects at QTL are smaller in the F6∶7 generation for all traits.

Key words

Zea mays RFLPs Plant breeding Genetics Recombination 



Restriction fragment length polymorphisms


quantitative trait loci


recombinant inbreds


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abler BS, Edwards MD, Stuber CW (1991) Isoenzymatic identification of quantitative trait loci in crosses of elite maize inbreds. Crop Sci 31:267–274Google Scholar
  2. Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459Google Scholar
  3. Austin DF (1995) Genetic analysis of quantitative trait loci with recombinant inbreds in maize. M.S. thesis, Iowa State University, Ames, IAGoogle Scholar
  4. Beavis WD, Grant D, Albertsen M, Fincher R (1991) Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic loci. Theor Appl Genet 83: 141–145PubMedGoogle Scholar
  5. Bubeck DM, Goodman MM, Beavis W D, Grant D (1993) Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci 33:838–847Google Scholar
  6. Burr B, Burr FA, Thompson KH, Albertsen MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526PubMedGoogle Scholar
  7. Cochran WG, Cox GM (1957) Experimental designs. John Wiley & Sons, New YorkGoogle Scholar
  8. Coe EH, Hoisington DA, Neuffer MG (1990) Linkage map of corn (maize). In: S.J. O'Brien (eds) Genetic maps, 5th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp 639–667Google Scholar
  9. Cowen NM (1988) The use of replicated progenies in marker-based mapping of QTLs. Theor Appl Genet 75:857–862Google Scholar
  10. Doebley J, Stec A (1991) Genetic analysis of the morphological differences between maize and teosinte. Genetics 129:285–295PubMedGoogle Scholar
  11. Doebley J, Stec A, Wendel J, Edwards M (1990) Genetic and morphological analysis of a maize-teosinte F2 population: implications for the origin of maize. Proc Natl Acad Sci USA 87:9888–9892Google Scholar
  12. Edwards MD, Page NJ (1994) Evaluation of marker-assisted selection through computer simulation. Theor Appl Genet 88:376–382Google Scholar
  13. Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125PubMedGoogle Scholar
  14. Edwards MD, Helentjaris T, Wright S, Stuber CW (1992) Molecular-marker-facilitated investigations of quantitative trait loci in maize. 4. Analysis based on genome saturation with isozyme and restriction fragment length polymorphism markers. Theor Appl Genet 83:765–774Google Scholar
  15. Freymark PJ, Lee M, Woodman WL, Martinson CA (1993) Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize (Zea mays L.). Theor Appl Genet 87:537–544Google Scholar
  16. Frova C, Gorla MS (1993) Quantitative expression of maize HSPs: genetic dissection and association with thermotolerance. Theor Appl Genet 86:213–220Google Scholar
  17. Gimelfarb A, Lande R (1994) Simulation of marker-assisted selection in hybrid populations. Genet Res 63:39–47Google Scholar
  18. Goldman IL, Rocheford TR, Dudley JW (1994) Molecular markers associated with maize kernel oil concentration in an Illinois high protein Illinois low protein cross. Crop Sci 34:908–915Google Scholar
  19. Haldane JBS (1919) The combination of linkage values and the calculation of distance between the loci of linked factors. J Genet 8:299–309Google Scholar
  20. Haldane JBS, Waddington CH (1931) Inbreeding and linkage. Genetics 16:357–374Google Scholar
  21. Hallauer AR, Miranda IB (1988) Quantitative genetics in maize breeding, 2nd edn. Iowa State University Press, Ames, IowaGoogle Scholar
  22. Knapp SJ, Bridges WC (1990) Using molecular markers to estimate quantitative trait locus parameters; power and genetic variances for unreplicated and replicated progeny. Genetics 126:769–777PubMedGoogle Scholar
  23. Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199PubMedGoogle Scholar
  24. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg L (1987) MAPMAKER: an interactive computer package for constructing primary linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  25. Matz EC, Burr FA, Burr B (1994) Molecular map based on TXCM and COXTX recombinant inbred families. Maize Genet Coop Newsl 68:198–208Google Scholar
  26. Ottaviano E, Gorla MS, Pé E, Frova C (1991) Molecular markers (RFLPs and HSPs) for the genetic dissection of thermotolerance in maize. Theor Appl Genet 81:713–719Google Scholar
  27. Pereira MG, Lee M (1995) Identification of genomic regions affecting plant height in sorghum and maize. Theor Appl Genet 90: 380–388Google Scholar
  28. Reiter RS, Coors IG, Sussman MR, Gableman WH (1991) Genetic analysis of tolerance to low-phosphorus stress in maize using restriction fragment length polymorphisms. Theor Appl Genet 82:561–568Google Scholar
  29. Sari-Gorla M, Pé ME, Mulcahy L, Ottaviano E (1992) Genetic dissection of pollen competitive ability in maize. Heredity 69:423–430Google Scholar
  30. SAS Institute Inc. (1987) SAS/STAT Guide for Personal Computers, Version 6 Edition, SAS Institute Inc. Cary, N.C.Google Scholar
  31. Schön CC, Lee M, Melchinger AE, Guthrie WD, Woodman WW (1993) Mapping and characterization of quantitative trait loci affecting resistance against second generation European corn borer in maize with the aid of RFLPs. Heredity 70:648–659Google Scholar
  32. Stuber CW, Edwards MD, Wendel IF (1987) Molecular-marker-facilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27:639–648Google Scholar
  33. Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839PubMedGoogle Scholar
  34. Veldboom LR, Lee M (1994) Molecular-marker facilitated studies of morphological traits in maize. II: Determination of QTLs for grain yield and yield components. Theor Appl Genet 88:7–16Google Scholar
  35. Veldboom LR, Lee M, Woodman WL (1994) Molecular marker-facilitated studies in an elite maize population: I. Linkage analysis and determination of QTL for morphological traits. Theor Appl Genet 88:7–16Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • D. F. Austin
    • 1
  • M. Lee
    • 1
  1. 1.Department of AgronomyIowa State UniversityAmesUSA

Personalised recommendations