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Cell and Tissue Research

, Volume 253, Issue 1, pp 189–198 | Cite as

Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain

  • Rae Silver
  • P. Witkovsky
  • P. Horvath
  • V. Alones
  • C. J. Barnstable
  • M. N. Lehman
Article

Summary

Cerebrospinal fluid-contacting (CSF) cells in both the septal and the tuberal areas in the brain of the ring dove are labeled by RET-P1, a monoclonal antibody to opsin that reacts with inner and outer segment membranes of rod photoreceptors in a variety of vertebrates. Immunoblot analysis of proteins from diverse brain regions, however, revealed bands of anti-RET-P1 immunoreactivity that did not correspond to opsin. Binding of RET-P1 to opsin-containing membranes, was not inhibited by membranes rich in muscarinic and β-adrenergic receptor proteins (red blood cells, heart, lung) taken from doves. RET-P1-immunoreactive CSF-contacting cells emit a dendritic process that penetrates the ependyma and ends in a knob-like terminal suspended in the ventricle. These cells also possess other processes that penetrate more or less deeply into the neuropil. Additionally, a band of labeled fibers occurs in the external layer of the median eminence. A double-label technique demonstrated that RET-P1-positive cells coexpress VIP-like immunoreactivity. VIP-positive cells in other brain areas are not RET-P1-positive.

Key words

Cerebrospinal fluid-contacting neurons Monoclonal antibodies Opsin VIP Extraocular photoreceptors Ring dove Coturnix coturnix Anas platyrhynchos 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Rae Silver
    • 1
  • P. Witkovsky
    • 2
  • P. Horvath
    • 1
  • V. Alones
    • 2
  • C. J. Barnstable
    • 3
  • M. N. Lehman
    • 4
  1. 1.Department of PsychologyBarnard College of Columbia UniversityNew YorkUSA
  2. 2.Department of OphthalmologyNew York UniversityNew YorkUSA
  3. 3.Rockefeller UniversityNew YorkUSA
  4. 4.Department of Anatomy and Cell BiologyUniversity of CincinnatiCincinnatiUSA

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