Theoretical and Applied Genetics

, Volume 91, Issue 5, pp 744–746 | Cite as

A comparison of male and female recombination frequency in wheat using RFLP maps of homoeologous group 6 and 7 chromosomes

  • G. Wang
  • V. Hyne
  • S. Chao
  • M. D. Gale
  • Y. Henry
  • J. De Buyser
  • J. W. Snape
Article

Abstract

A novel approach was used to compare male and female recombination rates in wheat. Doubled haploid lines were developed from an F1 using two distinct approaches: the anther-culture technique and the Hordeum bulbosum system, from which sets of lines were developed from “male” and “female” meioses, respectively. The genotype of the lines was established at RFLP and isozyme markers polymorphic on chromosomes of homoeologous groups 6 and 7, and “male” and “female” linkage maps were calculated using this information. The markers in one segment of chromosome 6B exhibited disturbed segregation frequencies in the anther-culture population. The “male” and “female” maps differed significantly in recombination frequency between some markers on two chromosomes, and these were consistent in direction within chromosomes and inconsistent in direction between chromosomes. In two of the four chromosomes studied the “male” map was much longer than the “female” map. These results suggest that significant differences may exist in male and female recombination frequencies in bread wheat which are specific to certain chromosomal segments but are inconsistent in direction between chromosomes. Other factors, such as environmental influences, may also be important in creating differences.

Key words

Wheat Anther culture Doubled haploid lines Recombination frequency Sex difference in recombination 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. Wang
    • 1
  • V. Hyne
    • 1
  • S. Chao
    • 2
  • M. D. Gale
    • 1
  • Y. Henry
    • 3
  • J. De Buyser
    • 3
  • J. W. Snape
    • 1
  1. 1.John Innes Centre, Norwich Research ParkColneyUK
  2. 2.Department of AgronomyUniversity of MissouriColumbiaUSA
  3. 3.Laboratoire de Biologie du Developpement des PlantesUniversite Paris XIOrsayFrance

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