Theoretical and Applied Genetics

, Volume 91, Issue 8, pp 1253–1256 | Cite as

Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method

  • S. Dumolin
  • B. Demesure
  • R. J. Petit


The restriction patterns of two chloroplast fragments and one mitochondrial DNA fragment, amplified by PCR with universal primers, were studied to determine the mode of inheritance of these organelles in 143 progeny of five intraspecific crosses in pedunculate oak (Quercus robur L.). The results indicate that both genomes are maternally inherited, an observation which agrees with the commonly observed pattern of inheritance in angiosperms. They confirm that both chloroplast DNA and mitochondrial DNA can be used as a source of seed-specific markers for the study of the geographic structure of oaks. This is the first report of organelle inheritance within the Fagaceae, an important and widespread tree family.

Key words

Insertion-deletion Maternal inheritance Non coding DNA Quercus robur Universal primers 


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  1. Bobolenz K, Nothnagel T, Metzlaff M (1990) Paternal inheritance of plastids in the genus Daucus. Mol Gen Genet 220:489–491Google Scholar
  2. Byrne M, Moran F, Tibbits WN (1993) Restriction map and maternal inheritance of chloroplast DNA in Eucalyptus nitens. J Hered 84:218–220Google Scholar
  3. Cruzan MB, Arnold ML, Carney SE, Wollenberg KR (1993) CpDNA inheritance in interspecific crosses and evolutionary inference in Louisiana Irises. Am J Bot 80:344–350Google Scholar
  4. Demesure B, Sodzi N, Petit RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol 4:129–131PubMedGoogle Scholar
  5. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  6. Erlich HA, Arnheim N (1992) Genetic analysis using the polymerase chain reaction. Annu Rev Genet 26:479–506Google Scholar
  7. Faure S, Noyer J-L, Carreel F, Horry J-P, Bakry F, Lanaud C (1994) Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata). Curr Genet 25:265–269Google Scholar
  8. Gyllensten U, Wharton D, Josefsson A, Wilson AC (1991) Paternal inheritance of mitochondrial DNA in mice. Nature 352:255–257Google Scholar
  9. Luro F (1993) Utilisation des marqueurs moléculaires pour la cartographie du génome et les études génétiques chez les agrumes. Thesis, University of Bordeaux II, FranceGoogle Scholar
  10. Masoud SA, Johnson LB, Sorensen EL (1990) High transmission of paternal plastid DNA in alfalfa plants demonstrated by restriction fragment polymorphic analysis. Theor Appl Genet 79:49–55Google Scholar
  11. Mejnartowicz M (1991) Inheritance of chloroplast DNA in Populus. Theor Appl Genet 82:477–480Google Scholar
  12. Metzlaff M, Börner T, Hagemann R (1981) Variations of chloroplast DNAs in the genus Pelargonium and their biparental inheritance. Theor Appl Genet 60:37–41Google Scholar
  13. Milligan BG (1992) Is organelle DNA strictly maternally inherited? Power analysis of a binomial distribution. Am J Bot 79:1325–1328Google Scholar
  14. Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial DNA are paternally inherited in Sequoia sempervirens. Proc Natl Acad Sci USA 86:9347–9349Google Scholar
  15. Palmer JD (1992) Comparison of chloroplast and mitochondrial genome evolution in plants. In: Herrmann R (ed) Cell organelles. Springer Verlag, Vienna Austria, pp 99–133Google Scholar
  16. Petit RJ, Kremer A, Wagner DB (1993a) Finite island model for organelle and nuclear genes in plants. Heredity 71:630–641Google Scholar
  17. Petit RJ, Kremer A, Wagner DB (1993b) Geographic structure of chloroplast DNA polymorphisms in European oaks. Theor Appl Genet 87:122–128Google Scholar
  18. Radetzky R (1990) Analysis of mitochondrial DNA and its inheritance in Populus. Curr Genet 18:429–434Google Scholar
  19. Rajora OP, Dancik BP (1992) Chloroplast inheritance in Populus. Theor Appl Genet 84:280–285Google Scholar
  20. Reboud X, Zeyl C (1994) Organelle inheritance in plants. Heredity 72:132–140Google Scholar
  21. Rushton BS (1993) Natural hybridization within the genus Quercus L. Ann Sci For, Suppl 1:73–90Google Scholar
  22. Shore JS, McQueen KL, Little SH (1994) Inheritance of plastid DNA in the Turnera ulmifolia complex (Turneraceae). Am J Bot 81:1636–1639Google Scholar
  23. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109Google Scholar
  24. Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM, Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc Natl Acad Sci USA 84:2097–2100PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. Dumolin
    • 1
  • B. Demesure
    • 1
  • R. J. Petit
    • 1
  1. 1.INRA, Laboratoire de génétique et d'amélioration des arbres forestiersGazinet CedexFrance

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