Theoretical and Applied Genetics

, Volume 91, Issue 2, pp 361–366 | Cite as

A gene encoding a truncated large subunit of Rubisco is transcribed and salt-inducible in rice

  • J.-S. Zhang
  • J. Gu
  • F.-H. Liu
  • S.-Y. Chen


Using the rice salt-tolerant mutant 20 as material, a cDNA library was constructed and two salt-inducible clones, SIR5.5 and SIR8.1, were isolated by differential screening. Homology analysis revealed that the two clones together constituted a chimeric rbcL which encoded a truncated large subunit of Rubisco with 337 amino-acids, plus 64 amino-acids of unknown origin. The expressions of both the normal and the chimeric locus appeared to be developmentally regulated and salt-inducible in shoots of the salt-tolerant mutant 20 and its original variety 77–170. In roots, their expressions were salt-inducible in the salt-tolerant mutant 20 whereas no, or only premature, forms were present in the salt-treated original variety 77–170. Higher concentrations of salt reduced the expressions of both normal rbcL and the chimeric locus. ABA showed no effect on their expression.

Key words

Oryza sativa Rice salt-tolerant mutant Chimeric rbcL Rubisco Salt stress 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • J.-S. Zhang
    • 1
  • J. Gu
    • 1
  • F.-H. Liu
    • 1
  • S.-Y. Chen
    • 1
  1. 1.Plant Biotechnology Laboratory, Institute of Genetics, Academia SinicaBeijingPeoples Republic of China

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