Theoretical and Applied Genetics

, Volume 91, Issue 2, pp 212–217 | Cite as

Cytological studies of African cultivated rice, Oryza glaberrima

  • N. Ohmido
  • K. Fukui


African cultivated rice, Oryza glaberrima Steud., was cytologically characterized by using both karyotype analysis and molecular cytology. The somatic chromosomes resemble those of Asian cultivated rice, Oryza sauva L., in general morphology, although some minor differences were noted. Multicolor fluorescence in situ hybridization (McFISH) with chromosomes detected one 45s (17s-5.8s-25s) ribosomal RNA gene locus (45s rDNA) and one 5s ribosomal RNA gene locus (5s rDNA) in the chromosome complement. The 45s rDNA and 5s rDNA loci were physically mapped to the distal end of the short arm of chromosome 9 and to the proximal region of the short arm of chromosome 11 respectively, as in O. sativa. Based on the cytological observations and the physical map of the rDNA loci, the chromosomal organization of O.glaberrima and O. sativa seems to be very similar.

Key words

Oryza glaberrima Karyotype analysis Ribosomal RNA genes Image analysis Multicolor in situ hybridization 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Clayton WD (1968) Studies in Gramineae. XVII. West african wild rice. Kew Bull 21:485–488Google Scholar
  2. Fukui K (1984) In situ hybridization. In: Uchmiya H, Tanaka K, Sugiura A (eds) Manual for plant genetic engineering. Kodansha Scientific, Tokyo, pp 165–168Google Scholar
  3. Fukui K (1986) Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet 72:27–32Google Scholar
  4. Fukui K (1988) Analysis and utility of chromosome information by using the chromosome image analyzing system, CHIAS. Bull Natl Inst Agrobiol Resour 4:153–175Google Scholar
  5. Fukui K, Iijima K (1991) Somatic chromosome map of rice by imaging methods. Theor Appl Genet 81:589–596Google Scholar
  6. Fukui K, Iijima K (1992) Manual on rice chromosomes. (2nd edn.) Misc Pub Natl Inst Agrobiol Resour 4:1–25Google Scholar
  7. Fukui K, Mukai Y (1988) Condensation pattern as a new image parameter for identification of small chromosomes in plants. Jpn J Genet 63:359–366Google Scholar
  8. Fukui K, Minezawa M, Kamisugi Y, Ohmido N, Ishikawa M, Yanagisawa T, Fujishita M, Sakai F (1992) Microdissection of plant chromosomes by argonion laser beam. Theor Appl Genet 84:787–791Google Scholar
  9. Fukui K, Kamisugi Y, Sakai F (1994a) Physical mapping of 5s rDNA loci by direct-cloned biotinylated probes in barley chromosomes. Genome 37:105–111Google Scholar
  10. Fukui K, Ohmido N, Khush GS (1994b) Variability in rDNA loci in genus Oryza detected through fluorescence in situ hybridization. Theor Appl Genet 87:893–899Google Scholar
  11. Hu CH (1960) Karyological studies in haploid rice plants. IV. Chromosome morphology and intragenome pairing in haploid plants of Oryza glaberrima Steud., as compared with those in O. sativa L. Cytologia 25:437–449Google Scholar
  12. Iijima K, Fukui F (1991) Clarification of the conditions for the image analysis of plant chromosomes. Bull Natl Inst Agrobiol Resour 6:1–58Google Scholar
  13. Iijima K, Kakeda K, Fukui K (1991) Identification and characterization of somatic rice chromosomes by imaging methods. Theor Appl Genet 81:597–605Google Scholar
  14. Ishiki K (1992) Cytogenetical studies on African rice, Oryza glaberrima Steud. 3. Primary trisomics produced by pollinating autotriploid with diploid. Euphytica 55:7–13Google Scholar
  15. Kamisugi Y, Fukui K (1990) Automatic karyotyping of plant chromosomes by imaging techniques. Biotechniques 8:290–295Google Scholar
  16. Kamisugi Y, Fukui K (1994) Fluorescent in situ hybridization using genes by direct cloning. In: Tanaka R (ed) Proc 2nd Sino-Japanese Symp Plant Chromosomes. International Academic Publishers, Beijing pp, 103–109Google Scholar
  17. Khush GS, Kinoshita T (1991) Rice karyotype, marker genes, and linkage groups. In: Khush GS, Toennieson GH (eds) Rice biotechnology. CAB International, Oxon, England, pp 83–108Google Scholar
  18. Kinoshita T (1991) Report of the committee on gene symbolization, nomenclature and linkage groups. Rice Genet Newslett 8:2–37Google Scholar
  19. Montogomery KT, Leblanc JM, Tsai P, Mcninch JS, Ward DC, Jong PJ, Kucherlapati R, Krauter KS (1993) Characterization of two chromosome-12 cosmid libraries and development of STSs from cosmids mapped by FISH. Genomics 17:682–693Google Scholar
  20. Morinaga T (1964) Cytogenetical investigations on Oryza species. In: IRRI (ed) Rice genetics and cytogenetics. Elsevier, Amsterdam, pp 91–110Google Scholar
  21. Morinaga T, Kuriyama, H (1957) Cytogenetical studies on Oryza sativa L. IX. The F1 hybrid of O. sativa L. and O. glaberrima Steud. Jpn J Breed 7:57–65Google Scholar
  22. Morishima H, Hinata K, Oka HI (1962) Comparison between two cultivated rice species, Oryza sativa L. and O. glaberrima Steud. Jpn J Breed 12:153–165Google Scholar
  23. Morishima H, Hinata K, Oka HI (1963) Comparison of models of evolution of cultivated forms from two wild rice species, Oryza breviligulata and O. perennis. Evolution 17:170–181Google Scholar
  24. Nakayama S, Fukui K (1994) Analysis and utility of chromosome information 64. Standardization of condensation pattern (CP) of plant chromosomes by image method. Abstracts 3rd Annu Meeting Bioimaging Soc, pp 54–55Google Scholar
  25. Ohmido N, Fukui K (1995) In situ hybridization method. In: National Agriculture Research Center (ed) The manual of rice breeding (in press)Google Scholar
  26. Ohtsubo H, Umeda M, Ohtsubo E (1991) Organization of DNA sequences highly repeated in tandem in rice genomes. Jpn J Genet 66:241–254Google Scholar
  27. Oka HI (1974) Experimental studies on the origin of cultivated rice. Genetics 78:475–486Google Scholar
  28. Oka HI (1988) Function and genetic bases of reproductive barriers. In: Oka HI (ed) Origin of cultivated rice. Japan Scientific Societies Press, Tokyo, pp 181–209Google Scholar
  29. Sano Y (1979) Gene markers obtained in Oryza glaberrima. Annu Rep Natl Inst Genet Jpn 29:97–98Google Scholar
  30. Sano Y (1985) Cytoplasm substitution of an Indica strain of Oryza sativa and O. glaberrima. Rice Genet Newslett 2:54Google Scholar
  31. Takaiwa F, Oono K, Sugiura M (1984) The complete nucleotide sequence of a rice 17s rRNA gene. Nucleic Acids Res 12:5441–5448Google Scholar
  32. Yabuno T (1977) Genetic studies on the interspecific cytoplasm substitution lines of japonica varieties of Oryza sativa L. and O. glaberrima Steud. Euphytica 26:451–463Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • N. Ohmido
    • 1
  • K. Fukui
    • 1
  1. 1.Hokuriku National Agricultural Experiment StationJoetsuJapan

Personalised recommendations