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Theoretical and Applied Genetics

, Volume 91, Issue 2, pp 212–217 | Cite as

Cytological studies of African cultivated rice, Oryza glaberrima

  • N. Ohmido
  • K. Fukui
Article

Abstract

African cultivated rice, Oryza glaberrima Steud., was cytologically characterized by using both karyotype analysis and molecular cytology. The somatic chromosomes resemble those of Asian cultivated rice, Oryza sauva L., in general morphology, although some minor differences were noted. Multicolor fluorescence in situ hybridization (McFISH) with chromosomes detected one 45s (17s-5.8s-25s) ribosomal RNA gene locus (45s rDNA) and one 5s ribosomal RNA gene locus (5s rDNA) in the chromosome complement. The 45s rDNA and 5s rDNA loci were physically mapped to the distal end of the short arm of chromosome 9 and to the proximal region of the short arm of chromosome 11 respectively, as in O. sativa. Based on the cytological observations and the physical map of the rDNA loci, the chromosomal organization of O.glaberrima and O. sativa seems to be very similar.

Key words

Oryza glaberrima Karyotype analysis Ribosomal RNA genes Image analysis Multicolor in situ hybridization 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • N. Ohmido
    • 1
  • K. Fukui
    • 1
  1. 1.Hokuriku National Agricultural Experiment StationJoetsuJapan

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