Pons, O. & Chaouche, K. Theoret. Appl. Genetics (1995) 91: 122. doi:10.1007/BF00220868
Nei's analysis of diversity at a diploid locus is extended to a population subdivided into a large number of subpopulations. The diversities and the heterozygotes frequency are defined with respect to the total population and unbiasedly estimated in a two-stage random cluster sampling. The fixation indices FIS, FIT andFST are derived, then inter- and intra-population variances of the estimated parameters are studied. We show that there is a unique sample size per population which yields the best accuracy in estimatingFST and FIS, respectively, at a given locus. These results are illustrated with an analysis of DNA diversity in a forest tree and compared to those obtained under the Hardy-Weinberg assumption.
Diversity Fixation indices Diploid locus Variance Optimal design