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Cell and Tissue Research

, Volume 158, Issue 4, pp 497–507 | Cite as

Prolactin and growth hormone cells in the human hypophysis: A study with immunoenzyme histochemistry and differential staining

  • N. S. Halmi
  • J. A. Parsons
  • S. L. Erlandsen
  • Theresa Duello
Article

Summary

Growth hormone and prolactin cells were immunostained in human hypophyses with antibody against rat growth hormone or prolactin and the peroxidase-antiperoxidase complex. Growth hormone cells were round and, in normal pituitaries, arranged in sizable groups. Prolactin cells occurred singly and were less numerous; they were often extensively branched. Only a few prolactin cells stained with carmoisine. Incubation of the antibody with an excess of the appropriate antigen greatly diminished or abolished immunostaining; absorption of anti-prolactin with growth hormone often enhanced it.

Prolactin cells were somewhat hypertrophied and hyperplastic in a neonate. Many of them stained with carmoisine.

An even greater hypertrophy and hyperplasia of these cells (which pushed apart the growth hormone cells) was found in a lactating woman. Immunostained giant prolactin cells were also observed. Staining of the prolactin cells with carmoisine was extensive. Upon prolonged exposure to anti-growth hormone antibody, ACTH/MSH cells also showed immunostaining which was abolished by absorption of the antiserum with growth hormone but not with synthetic 1–24ACTH.

Growth hormone cells evidently correspond to the α acidophils of Romeis, prolactin cells in lactation to his η cells; the relation of his ɛ cells to the pleomorphic “resting” prolactin cells is not clear.

Key words

Hypophysis Man Prolactin Growth hormone Immunostaining 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • N. S. Halmi
    • 1
  • J. A. Parsons
    • 1
  • S. L. Erlandsen
    • 1
  • Theresa Duello
    • 1
  1. 1.Department of AnatomyUniversity of IowaIowa CityUSA

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