Cell and Tissue Research

, Volume 209, Issue 1, pp 161–166 | Cite as

Thyrotropes in the pituitary are target cells for 1,25 dihydroxy vitamin D3

  • Madhabananda Sar
  • Walter E. Stumpf
  • Hector F. DeLuca
Article

Summary

In the anterior pituitary of the rat, target cells of 1,25 (OH)2 vitamin D3 are identified as those that secrete thyroid stimulating hormone by means of a combined technique of thaw-mount autoradiography and immunohistochemistry. The results for the first time provide evidence that suggests a central effect of 1,25 (OH)2 vitamin D3 on the modulation of thyrotropin secretion in a manner similar to that of other steroid hormones at the level of the pituitary.

Key words

1,25 (OH)2 vitamin D3 Thyrotropes Autoradiography Immunohistochemistry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chertow B, Baylink BD, Wergedal J, Su M, Norman A (1975) Decrease in serum immunoreactive parathyroid hormone in rats and in parathyroid hormone secretion in vitro 1,25-dihydroxycholecalciferol. J Clin Invest 56:668–678Google Scholar
  2. DeLuca HF (1977) Vitamin D metabolism. Clin Endocrinol 7:Suppl 1 S-17 SGoogle Scholar
  3. Frolik CA, DeLuca HF (1973) The stimulation of 1,25-dihydroxycholecalciferol metabolism in vitamin D-deficient rats by 1,25-dihydroxycholecalciferol treatment. J Clin Invest 52:543–548Google Scholar
  4. Garabedian M, Holick MF, DeLuca HF, Boyle IT (1972) Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. Proc Natl Acad Sci (Wash.) 69:1673–1676Google Scholar
  5. Glanzmann E (1934) Endokrine Drüsen und Rachitis. Zeitschrift für Vitaminforschung. 3:167–195Google Scholar
  6. Haussler MR, Rasmussen H (1972) Metabolism of vitamin D3 in chick. J Biol Chem 247:2328–2335Google Scholar
  7. Henry H, Norman A (1975) Studies in the mechanism of action of calciferol VII localization of 1,25-dihydroxy-vitamin D3 in chick parathyroid gland. Biochem Biophys Res Commun 62:781–788Google Scholar
  8. Henry H, Norman A, Taylor AN, Hartenbower DL, Couburn JW (1976) Biological activity 24,25 dihydroxycholecalciferol in chicks and rats. J Nutr 106:724–734Google Scholar
  9. Holick M, Schnoes HK, DeLuca HF, Suda T, Cousins RJ (1971) Isolation and identification of 1,25-dihydroxycholecalciferol. A metabolite of vitamin D active in the intestine. Biochemistry 10:2799–2804Google Scholar
  10. Nitzesco II, Bratiano S (1936) Les effects des fortes doses de vitamin D sur la structure histologique des glandes à sécretion interne. Comptes Rendus Hebdomadaires des Séances et Memoires de la Societé de Biologie 1:1533–1535Google Scholar
  11. Pavlovitch H, Presle V, Balsan S (1977) Decreased bone sensitivity of thyroidectomized rats to the calcaemic effect of 1,25 dihydroxycholecalciferol. Acta Endocrinol 84:774–779Google Scholar
  12. Raisz LG, Trammel CL, Holick MF, DeLuca HF (1972) 1,25-dihydroxycholecalciferol: A potent stimulator of bone resorption in tissue culture. Science 175:768–769Google Scholar
  13. Reynolds JJ, Holick MF, DeLuca HF (1973) The role of vitamin D metabolites in bone resorption. Cell Tissue Res 12:295–301Google Scholar
  14. Sar M, Stumpf WE (1979) Simultaneous localization of steroid and peptide hormones in rat pituitary by combined thaw-mount autoradiography and immunohistochemistry: Localization of dihydrotestosterone in gonadotropes, thyrotropes and pituicytes. Cell Tissue Res 203:1–7Google Scholar
  15. Stumpf WE, Sar M (1975) Autoradiographic techniques for localizing steroid hormones. In: BW O'Malley, and JG Hardman (eds) Methods in Enzymology, Vol 36, pp 135–156. Academic Press, New YorkGoogle Scholar
  16. Stumpf WE, Sar M (1976) Autoradiographic localization of estrogen, androgen, progestin and glucocorticosteroid in “target tissues” and “nontarget tissues”. In: JR Pasqualini (ed) Receptors and mechanism of action of steroid hormones, Part 1, pp 41–84. Marcel Dekker, New YorkGoogle Scholar
  17. Stumpf WE, Sar M, Reid FA, Tanaka Y, DeLuca HF (1979) Target cells for 1,25-dihydroxy-vitamin D3 in intestinal tract, stomach, kidney, skin, pituitary and parathyroid. Science 206:1188–1190Google Scholar
  18. Suda T, DeLuca HF, Tanaka Y (1970) Biological activity of 25-hydroxyergocalciferol in rats. J Nutr 100:1049–1052Google Scholar
  19. Wasserman RH, Corradino RA, Fullmer CS, Taylor AN (1974) Some aspects of vitamin D action: Calcium absorption and the vitamin D-dependent calcium binding protein. Vitamins and Hormones 32:299–324Google Scholar
  20. Wecksler WR, Henry HL, Norman AW (1977) The subcellular localization of 1, 25-dihydroxy vitamin D3 in the parathyroid glands of chicken. In: AW Norman, K Schaefer, JW Coburn, HF DeLuca, D Fraser, HG Grigolett, DV Herrath (eds) Vitamin D. Biochemical and Clinical Aspects Related to Calcium Metabolism, pp 219–229. Walter De Gruyter, Berlin-New YorkGoogle Scholar
  21. Zisman E, Lotz M, Jenkins ME, Bartter FC (1969) Studies in pseudohypoparathyroidism. Two new cases with a probably selective deficiency of thyrotropin. Am J Med 46:469–471Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Madhabananda Sar
    • 1
  • Walter E. Stumpf
    • 1
  • Hector F. DeLuca
    • 1
    • 2
  1. 1.Departments of Anatomy and PharmacologyUniversity of North CarolinaChapel HillUSA
  2. 2.Department of BiochemistryUniversity of WisconsinMadisonUSA
  3. 3.Department of AnatomyUniversity of North CarolinaChapel HillUSA

Personalised recommendations