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Human Genetics

, Volume 92, Issue 2, pp 183–188 | Cite as

Affinity differences for vitamin D metabolites associated with the genetic isoforms of the human serum carrier protein (DBP)

  • J. Arnaud
  • J. Constans
Original Investigations

Abstract

Human vitamin D binding protein (DBP) displays considerable polymorphism with 120 described alleles. Among these, three alleles are frequently observed, Gc 1F (pI 4.94–4.84), Gc 1S (pI 4.95–4.85) and Gc 2 (pI 5.1). Differences between these genetic forms of the protein in affinity for vitamin D metabolites have been detected by electrophoretic methods. The constant affinity (Ka) values determined in this study confirm these differences. The affinities of six rare variants were also examine. Those of the DBP genetic forms to the vitamin D derivatives 25-OH-D3 and 1,25-(OH)2-D3 seem to be related to the isoelectric point of the proteins: a high affinity corresponding to a low isoelectric point. The Gc 1A9 and 1A11 mutants were associated with higher affinity for the vitamin D derivatives and the Gc 1C1 and 1C21 mutants were deficient.

Keywords

Isoelectric Point Rare Variant Constant Affinity Genetic Form Electrophoretic Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • J. Arnaud
    • 1
  • J. Constans
    • 2
  1. 1.CRPG — CNRS, CHU PurpanToulouseFrance
  2. 2.CRTS Laboratoire d'Hemostase, CHU PurpanToulouseFrance

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