Cell and Tissue Research

, Volume 169, Issue 3, pp 395–403 | Cite as

Fine structure of taste buds in the barbel of the catfish, Ictalurus punctatus

  • Nicola Grover-Johnson
  • Albert I. Farbman
Article

Summary

Taste buds occur in the epithelium of the catfish barbel along its entire length. Two major cell types, light and dark cells, occupy the upper two-thirds of the taste bud. A third cell type, the basal cell, lies on the basal lamina and is essentially separated from the light and dark cells by a plexus of unmyelinated nerve fibers. The dark cells have branching processes, both apically and basally whereas the light cells have a single apical process and many basal processes. The apical processes of dark cells contain secretory granules, while the apical processes of light cells contain an abundant agranular endoplasmic reticulum. Light cell nuclei contain bundles of 10 nm filaments, often arranged in the shape of a cup or ring, but nucleoli are rarely seen. It is suggested that this morphology indicates a low degree of RNA synthesis by light cells. The basal cells contain large numbers of vesicles, about 60 nm in diameter, which are sometimes seen in clumps in relation to an adjacent nerve fiber in a configuration resembling a synapse. Curiously, although basal cells present a large surface to the basal lamina, there are no hemidesmosomes. This suggests that the basal cell does not originate from the epidermis.

Key words

Taste buds Fishes Electron microscopy Nucleolus 

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References

  1. Crisp, M., Lowe, G.A., Laverack, M.S.: On the ultrastructure and permeability of taste buds of the marine teleost, Ciliata mustela. Tiss. and Cell 7, 191–202 (1975)Google Scholar
  2. Desgranges, M.J.: Sur l'existence de plusieurs types de cellules sensorielles dans les bourgeons du gout des barbillons du Poisson-chat. C. R. Acad. Sc. (Paris) 261, 1095–1106 (1965)Google Scholar
  3. Desgranges, M.J.: Sur la double innervation des cellules sensorielles des bourgeons du gout des barbillons du Poisson-chat. C. R. Acad. Sci. (Paris) Ser. D 263, 1103–1106 (1966)Google Scholar
  4. Desgranges, M.J.: Sur les bourgeons du gout du Poisson-chat Ictalurus melas: Ultrastructures des cellules basales. C. R. Acad. Sci. (Paris) Ser. D 272, 1814–1817 (1972)Google Scholar
  5. Farbman, A.I.: Fine structure of the taste bud. J. Ultrastruct. Res. 12, 328–350 (1965)Google Scholar
  6. Farbman, A.I.: Structure of chemoreceptors. In: Symposium on Foods; Physiology and Chemistry of Flavors. (H.W. Schultz, E.A. Day, L.M. Libbey, eds.) pp. 25–51. Avi Publishing Co. 1967Google Scholar
  7. Farbman, A.I., Yonkers, J.D.: Fine structure of the taste bud in the mud puppy, Necturus maculosus. Amer. J. Anat. 131, 353–370 (1971)Google Scholar
  8. Gray, E.G., Watkins, K.C.: Electron microscopy of taste buds in the rat. Z. Zellforsch. 66, 583–595 (1965)Google Scholar
  9. Heidenhain, M.: Über die Sinnesfelder und die Geschmacksknospen der Papilla foliata des Kaninchens. Arch. mikr. Anat. 85, 365–479 (1914)Google Scholar
  10. Hirata, V.: Fine structure of terminal buds on the barbels of some fishes. Arch. Histol. Jap. 26, 507–523 (1966)Google Scholar
  11. Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137a (1965)Google Scholar
  12. Lane, N.J.: Intranuclear fibrillar bodies in actinomycin D-treated oocytes. J. Cell Biol. 40, 286–291 (1969)Google Scholar
  13. Nemetschek-Gansler, H., Ferner, H.: Über die Ultrastruktur der Geschmacksknospen. Z. Zellforsch. 63, 155–178 (1964)Google Scholar
  14. Olmsted, J.M.D.: The results of cutting the seventh cranial nerve in Ameiurus nebulosus (Leseur). J. exp. Zool. 31, 369–401 (1920)Google Scholar
  15. Rajbanshi, V.K., Tewari, H.B.: Structure of the taste bud of Saccobranchus fossilis. Z. Biol. 116, 22–28 (1968)Google Scholar
  16. Reutter, K.: Die Geschmacksknospen des Zwergwelses Ameiurus nebulosus (Leseur). Morphologische und histochemische Untersuchungen. Z. Zellforsch. 120, 280–308 (1971)Google Scholar
  17. Scalzi, H.A.: The cytoarchitecture of gustatory receptors from the rabbit foliate papillae. Z. Zellforsch. 80, 413–435 (1967)Google Scholar
  18. Stensaas, L.J.: The fine structure of fungiform papillae and epithelium of the tongue of a South American toad, Calyptocephalella gayi. Amer. J. Anat. 131, 443–462 (1971)Google Scholar
  19. Uga, S., Hama, K.: Electronmicroscopic studies on the synaptic region of the taste organ of carps and frogs. J. Electron Micros. 16, 269–277 (1967)Google Scholar
  20. Welsch, U., Storch, V.: Die Feinstruktur der Geschmacksknospen von Welsen [Clarias batrachus (L.) und Kryptopteros bicirrhis (Cuvier et Valenciennes)]. Z. Zellforsch. 100, 552–559 (1969)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Nicola Grover-Johnson
    • 1
  • Albert I. Farbman
    • 1
  1. 1.Department of AnatomyNorthwestern UniversityChicagoUSA

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