Advertisement

Molecular and Cellular Biochemistry

, Volume 73, Issue 2, pp 141–155 | Cite as

Ontogeny of adenosine 3′,5′-monophosphate metabolism in guinea pig cerebral cortex

I. Development of responses to histamine, norepinephrine and adenosine
  • R. F. Shonk
  • T. W. Rall
Original Articles

Abstract

The effects of norepinephrine, histamine and adenosine, singly or in combinations, on the accumulation of adenosine 3′,5′-monophosphate were examined in slices of cerebral cortex from strain 2 guinea pigs at 40 to 68 days of gestation. The response to histamine was 2-fold at 40 days, increased to 19-fold at 55 days and declined there after toward the adult value of 4-fold. The response to adenosine was first apparent at 44 days and developed rapidly to a maximum of about 40-fold at 55 days. The response to norepinephrine remained at about 2-fold throughout the entire period. Synergistic responses to combinations of pairs of agents all became visible at 42 days and the degree of synergism was maximal by 47 to 48 days of gestation. The pharmacological characteristics of responses in fetal tissue resembled those in adult tissue in that the effects of norepinephrine in the presence of either adenosine or histamine were mediated principally by α-adrenergic receptors and the responses to histamine were more effectively inhibited by H1 and H2 antagonists in the presence and absence of adenosine, respectively.

Keywords

cyclic AMP fetal guinea pig brain adenosine histamine norepinephrine ontogeny 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Daly JW: Cyclic Nucleotides in the Nervous System. Plenum Press, New York, 1977, pp 104–130.Google Scholar
  2. 2.
    Dismukes K, Rogers M, Daly JW: Cyclic adenosine 3′,5′-monophosphate formation in guinea pig brain slices: Effect of H1- and H2-histaminergic agonists. J Neurochem 26:785–790, 1976.Google Scholar
  3. 3.
    Dobbing J, Sands J: Growth and development of the brain and spinal cord of the guinea pig. Brain Res 17:115–123, 1970.Google Scholar
  4. 4.
    Flexner LB: Enzymatic and functional paterns of the developing mammalian brain. In: Waelsh H (Ed). Biochemistry of the Developing Nervous System. Academic press, New York, 1955, pp 281–295.Google Scholar
  5. 5.
    Gilman AG: A protein binding assay for adenosine 3′,5′-cyclic monophosphate. Proc Natl Acad Sci USA 67:305–312, 1970.Google Scholar
  6. 6.
    Hill SJ, Daum P, Young JM: Affinities of histamine H1-antagonists in guinea pig brain: similarity of values determined from [3H] mepyramine binding and from inhibition of a functional response. J Neurochem 37:1357–1360, 1981.Google Scholar
  7. 7.
    Huang M, Shimizu H, Daly JW: Regulation of adenosine cyclic 3′,5′-monophosphate formation in cerebral cortical slices: Interaction among norepinephrine, histamine and serotonin. Mol Pharmacol 7:155–162, 1971.Google Scholar
  8. 8.
    Jones DG, Dittmer MM, Reading LC: Synaptogenesis in guinea pig cerebral cortex: A glutaraldehyde-PTA study. Brain Res 70:245–259, 1974.Google Scholar
  9. 9.
    Jones DJ, McKenna LF: Alpha adrenergic receptor mediated formation of cyclic AMP in rat spinal cord. J Cyclic Nucleotide Res 6:133–141, 1980.Google Scholar
  10. 10.
    Kakiuchi S, Rall TW: The influence of chemical agents on the accumulation of adenosine 3′,5′-phosphate in slices of rabbit cerebellum. Mol Pharmacol 4:367–378, 1968.Google Scholar
  11. 11.
    Kakiuchi S, Rall TW, McIlwain H: The effect of electrical stimulation upon the accumulation of adenosine 3′,5′-phosphate in isolated cerebral tissue. J Neurochem 16:485–491, 1969.Google Scholar
  12. 12.
    Lohmann SM, Ueda T, Greengard P: Ontogeny of synaptic phosphoproteins in brain. Proc Natl Acad Sci USA 75:4037–4041, 1978.Google Scholar
  13. 13.
    Lowry OH, Rosebrough NJ, Farr AL, Randall L: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951.PubMedGoogle Scholar
  14. 14.
    McCarthy KD, de Vellis J: Alpha-adrenergic receptor modulation of beta-adrenergic, adenosine and prostaglandin E1 increased adenosine 3′:5′-cyclic monophosphate levels in primary cultures of glia. J Cyclic Nucleotide Res 4:15–26, 1978.Google Scholar
  15. 15.
    Palacios JM, Garbarg M, Barbin G, Schwartz JC: Pharmacological characterization of histamine receptors mediating the stimulation of cyclic AMP accumulation in slices from guinea-pig hippocampus. Mol Pharmacol 14:971–982, 1978.Google Scholar
  16. 16.
    Perkins JP, Moore MM: Characterization of the adrenergic receptors mediating a rise in cyclic 3′,5′-adenosine monophosphate in rat cerebral cortex. J Pharmacol Exp Ther 185:371–378, 1973.Google Scholar
  17. 17.
    Perkins JP, Moore MM: Regulation of the adenosine cyclic 3′,5′-monophosphate content of rat cerebral cortex: Ontogenetic development of the responsiveness to catecholamines and adenosine. Mol Pharmacol 9:774–782, 1973.Google Scholar
  18. 18.
    Peters VB, Flexner LB: Biochemical and physiological differentiation during morphogenesis. VIII. Quantitative morphologic studies on the developing cerebral cortex of the fetal guinea pig. Am J Anat 86:133–162, 1950.Google Scholar
  19. 19.
    Premont J, Perez M, Bochaert J: Adenosine-sensitive adenylate cyclase in rat striatal homogenates and its relationship to dopamine- and Ca++-sensitive adenylate cyclase. Mol Pharmacol 13:662–670, 1977.Google Scholar
  20. 20.
    Rall TW: Regulation of cyclic adenosine monophosphate accumulation in brain tissue: Interaction of adenosine with other agonists. In: Baer HP, Drummond GI (eds). Physiological and Regulatory Functions of Adenosine and Adenine Nucleotides. Raven Press, New York, 1979, pp 217–227.Google Scholar
  21. 21.
    Rall TW, Lehne RA: Evidence for cross-linking of cyclic AMP to constituents of brain tissue by aldehyde fixatives: Potential utility in histochemical procedures. J Cyclic Nucleotide Res 8:243–265, 1982.Google Scholar
  22. 22.
    Rogers M, Dismukes K, Daly JW: Histamine-elicited accumulations of cyclic adenosine 3′,5′-monophosphate in guinea pig brain slices: Effect of H1- and H2-antagonists. J Neurochem 25:531–534, 1975.Google Scholar
  23. 23.
    Sano M, Kitajima S: Ontogeny of calmodulin and calmodulin-dependent adenylate cyclase in rat brain. Dev Brain Res 7:215–220, 1983.Google Scholar
  24. 24.
    Sano M, Kitajima S, Mizutani A: Activation of adenylate cyclase by forskolin in rat brain and testis. Arch Biochem Biophys 220:333–339, 1983.Google Scholar
  25. 25.
    Sattin A, Rall TW: The effect of adenosine and adenine nucleotides on the cyclic adenosine 3′,5′-phosphate content of guinea pig cerebral cortical slices. Mol Pharmacol 6:13–23, 1970.Google Scholar
  26. 26.
    Sattin A, Rall TW, Zanella J Jr: Regulation of cyclic adenosine 3′,5′-monophosphate levels in guinea pig cerebral cortex by interaction of alpha-adrenergic and adenosine receptor activity. J Pharmacol Exp Ther 192:22–32, 1975.Google Scholar
  27. 27.
    Schmidt MJ, Robison GA: The effect of norepinephrine on cyclic AMP levels in discrete regions of the developing rabbit brain. Life Sci 10(1):459–464, 1971.Google Scholar
  28. 28.
    Schultz J: Cyclic adenosine 3′,5′-monophosphate in guinea pig cerebral cortical slices: Possible regulation of phosphodiesterase activity by cyclic adenosine 3′,5′-monophosphate and calcium ions. J Neurochem 24:495–501, 1975.Google Scholar
  29. 29.
    Schultz J, Daly JW: Adenosine 3′,5′-monophosphate in guinea pig cerebral cortical slices: Effects of α- and β-adrenergic agents, histamine, serotonin and adenosine. J Neurochem 21:573–579, 1973.Google Scholar
  30. 30.
    Strada SJ, Uzunov P, Weiss B: Ontogenetic development of a phosphodiesterase activator and the multiple forms of cyclic AMP phosphodiesterase of rat brain. J Neurochem 23:1097–1104, 1974.Google Scholar
  31. 31.
    Weiss B: Ontogenetic development of adenyl cyclase and phosphodiesterase in rat brain. J Neurochem 18:469–477, 1971.Google Scholar
  32. 32.
    Zanella J Jr, Rall TW: Evaluation of electrical pulses and elevated levels of potassium ions as stimulants of adenosine 3′,5′-monophosphate (cyclic AMP) accumulation in guinea pig brain. J Pharmacol Exp Ther 186:241–252, 1973.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • R. F. Shonk
    • 1
    • 2
  • T. W. Rall
    • 1
    • 2
  1. 1.Department of PharmacologyCase Western Reserve UniversityCleveland
  2. 2.Department of PharmacologyUniversity of VirginiaCharlottesvilleUSA

Personalised recommendations