Assignment of the human CRABP-II gene to chromosome 1q21 by nonisotopic in situ hybridization
- Cite this article as:
- Elder, J.T., Åström, A., Pettersson, U. et al. Hum Genet (1992) 89: 487. doi:10.1007/BF00219171
- 24 Downloads
Two highly conserved forms of cellular retinoic acid binding protein (CRABP-I and CRABP-II) have been described, and one, CRABP-II, is highly expressed in human skin. We have utilized a 10-kb fragment containing the human CRABP-II (hCRABP-II) gene (isolated from a human genomic library) to localize hCRABP-II to human chromosome 1 band q21 by fluorescence in situ hybridization. Localization to 1q was confirmed by hybridization of a hCRABP-II cDNA clone against a human-mouse hybrid cell line containing a t(1;6)(q21;q13) translocation chromosome. The hCRABP-II gene is therefore localized to a band known to contain several other genes that are expressed in the context of epidermal differentiation, including profilaggrin, loricrin, involucrin, and calcyclin.