Cell and Tissue Research

, Volume 178, Issue 2, pp 199–219 | Cite as

Origin, destination and mapping of tritocerebral neurons of locust

  • Elisabeth Aubele
  • Nikolai Klemm


The connectivities of the tritocerebrum of locust (Locusta migratoria L., Schistocerca gregaria (Forsk.)) were studied histologically and by means of cobalt chloride infusion. Its neuropil consists partly of fibers which traverse the tritocerebrum and areas consisting of neuropilar agglomerizations (“glomeruli”). The following direct connections between the tritocerebrum and other regions were observed: connections to 1) dorsal and lateral brain regions (mushroom body, optic lobe), 2) the ventral nerve cord, 3) the stomatogastric nervous system (here the protocerebrum and the subesophageal ganglion are also involved in these connections), 4) the retro-cerebral glands (corpora cardiaca, corpora allata), and 5) muscles of the foregut.

Key words

Locust brain Tritocerebrum Frontal ganglion Neurohistology Cobalt infusion 


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  1. Achtelig, M.: Über die Anatomie des Kopfes von Raphidia flavipes Stein und die Verwandschafts-beziehungen der Raphidiidae zu den Megalopteren. Zool. Jb. Anat. 84, 249–312 (1967)Google Scholar
  2. Albrecht, F.O.: The anatomy of the migratory locust. London: Athlone 1953Google Scholar
  3. Bordas, L.: Contribution à l'étude du système nerveux sympathique intestinal ou stomatogastrique des Orthoptères. Bull. Sci. France et Belgique 33, 458–482 (1900)Google Scholar
  4. Bullock, T.H., Horridge, G.A.: Structure and function in the nervous system of invertebrates. Vol. I and II. San Francisco and London: Freeman 1965Google Scholar
  5. Cassier, P., Fain-Maurel, M.-A.: Contribution à l'étude infrastructural du système neurosécréteur retrocérébrale chez Locusta migratoria migratorioides (R. et F.). Le transit des neurosécrétions. Z. Zellforsch. 111, 483–492 (1970)Google Scholar
  6. Clarke, K.U., Langley, P.A.: Studies on the initiation of growth and moulting in Locusta migratoria migratorioides R. & F. -IV. The relationship between the stomatogastric nervous system and neurosecretion. J. Insect Physiol. 9, 423–430 (1963)Google Scholar
  7. Cooter, R.J.: Ocellus and ocellar nerves of Periplaneta americana L. (Orthoptera: Dictioptera). Int. J. Insect Morphol. embryol. 4, 273–288 (1975)Google Scholar
  8. Davey, K.G.: The nervous pathway involved in the release by feeding of a pharmacologically active factor from the corpus cardiacum of Periplaneta. J. Insect Physiol. 8, 579–583 (1962)Google Scholar
  9. Girardie, A.: Neurosécrétions cérébrales chez les Acridiens. Bull. Soc. Zool. France 95, 783–802 (1970)Google Scholar
  10. Girardie, J.: Recherche en microscopie photonique et électronique des éléments neurosécréteurs tritocérébraux de Locusta migratoria (Insecte Orthoptère). Arch. anat. micr. Morph. exp. 64, 223–246 (1976)Google Scholar
  11. Goodman, C.: Anatomy of ocellar interneurones: constancy and variability. J. comp. Physiol. 95, 185–201 (1974)Google Scholar
  12. Goodman, C., Williams, J.L.D.: in pressGoogle Scholar
  13. Goodman, L.J., Patterson, J.A., Mobbs, P.G.: The projection of ocellar neurons within the brain of the locust, Schistocerca gregaria. Cell Tiss. Res. 137, 467–492 (1975)Google Scholar
  14. Hanström, B.: Vergleichende Anatomie des Nervensystems der wirbellosen Tiere. Berlin: Springer 1928Google Scholar
  15. Hanström, B.: Inkretorische Organe, Sinnesorgane und Nervensystem des Kopfes einiger niederer Insektenordnungen. Kungl. Svenska Vetensk. Akad. Handl., Ser. 3B, 4–266 (1940)Google Scholar
  16. Highnam, K.C.: The histology of the neurosecretory system of the adult female desert locust, Schistocerca gregaria. Quart. J. micr. Sci. 102, 27–38 (1961)Google Scholar
  17. Honegger, H.-W., Schürmann, F.W.: Cobalt sulphide staining of optic fibres in the brain of the cricket, Gryllus campestris. Cell Tiss. Res. 159, 213–225 (1975)Google Scholar
  18. Jawlowski, H.: On the origin of corpora pedunculata and the structure of the tuberculum opticum (Insecta). Acta anat. (Basel) 53, 346–359 (1963)Google Scholar
  19. Klemm, N.: Die Morphologie des Kopfes von Ryacophila Pict. (Trichoptera). Zool. Jb. Anat. 83, 1–51 (1966)Google Scholar
  20. Klemm, N.: Monoaminhaltige Strukturen im Zentralnervensystem der Trichoptera (Insecta). Teil I. Z. Zellforsch. 92, 487–502 (1968)Google Scholar
  21. Klemm, N.: Monoaminhaltige Strukturen im Zentralnervensystem der Trichoptera (Insecta). Teil II. Z. Zellforsch. 117, 537–558 (1971)Google Scholar
  22. Klemm, N.: Vergleichend-histochemische Untersuchungen über die Verteilung monoamin-haltiger Strukturen im Oberschlundganglion von Angehörigen verschiedener Insektenordnungen. Ent. germ. 1, 21–49 (1974)Google Scholar
  23. Klemm, N.: Histochemistry of putative transmitter substances in the insect brain. Progr. Neurobiol. 7, 99–169 (1976)Google Scholar
  24. Klemm, N., Axelsson, S.: Determination of dopamine, noradrenaline, 5-hydroxytryptamine in the cerebral ganglion of the desert locust, Schistocerca gregaria Forsk. (Insecta, Orthoptera). Brain Res. 57, 289–298 (1973)Google Scholar
  25. Klemm, N., Falck, B.: Monoamines in the pars intercerebralis-corpus cardiacum-complex in locust. (In press)Google Scholar
  26. Maddrell, S.H.P.: Neurosecretion in insects. In: Insect and physiology (J.W.L. Beament and J.E. Treherne, eds.), pp. 103–118. Edinburgh: Oliver and Boyd 1967Google Scholar
  27. Mason, C.A.: New features of the brain-retrocerebral neuroendocrine complex of the locust Schistocerca vaga (Scudder). Z. Zellforsch. 141, 19–32 (1973)Google Scholar
  28. Maynard, D.M.: Organization of neuropil. Amer. Zoologist 2, 79–96 (1962)Google Scholar
  29. Miller, T.A.: Neurosecretion and the control of visceral organs in insects. Ann. Rev. Entomol. 20, 133–149 (1975)Google Scholar
  30. Möhl, B.: The control of foregut movements by the stomatogastric nervous system in the European house cricket Acheta domesticus L. J. comp. Physiol. 80, 1–28 (1972)Google Scholar
  31. Orlov, J.: Die Innervation des Darmes der Insekten (Larven von Lamellicorniern). Z. wiss. Zool. 122, 425–502 (1924)Google Scholar
  32. Orlov, J.: Über den histologischen Bau der Ganglien des Mundmagennervensystems der Insekten. Z. mikr.-anat. Forsch. 2, 35–110 (1925)Google Scholar
  33. Orlov, J.: Über die Innervation des Schlundes bei Arthropoda. Z. mikr.-anat. Forsch. 20, 98–106 (1930)Google Scholar
  34. O'Shea, M., Rowell, C.H.F., Williams, J.L.D.: The anatomy of a locust visual interneurone; the descending contralateral movement detector. J. exp. Biol. 60, 1–12 (1974)Google Scholar
  35. Penzlin, H.: Zur Rolle des Frontalganglions bei Larven der Schabe Periplaneta americana. J. Insect Physiol. 17, 559–573 (1971)Google Scholar
  36. Plotnikova, S.I.: Vegetative elements in the supraoesophageal ganglion in insects. Zh. evol. biochim. fiziol. 1, 307–310 (1965) [Russian]Google Scholar
  37. Plotnikova, S. I.: The structure of sympathetic nervous system of insects. Symposion on Neurobiology of Invertebrates, 59–68, Budapest 1967Google Scholar
  38. Plotnikova, S.I.: The structure of sympathetic centres in insect nervous system. Tr. vses. entomol. o.-va 53, 36–59 (1969) [Russian]Google Scholar
  39. Plotnikova, S.I., Govyrin, V.A.: Distribution of catecholamine-containing nerve elements in some representatives of Protostomia and Coelenterata. Arch. anat. gistol. embriol. 50, 79–87 (1966) [Russian]Google Scholar
  40. Politoff, A., Pappas, G.D., Bennett, M.V.L.: Cobalt ions cross an electric synapse if cytoplasmic concentration is low. Brain Res. 76, 343–346 (1974)Google Scholar
  41. Robertson, H.A.: The innervation of the salivary gland of the moth, Manduca sexta. Cell Tiss. Res. 148, 237–245 (1974)Google Scholar
  42. Romeis, B.: Mikroskopische Technik. München-Wien: Oldenbourg 1968Google Scholar
  43. Rowell, C.H.F.: Cited in addendum in Strausfeld and Obermayer (1976)Google Scholar
  44. Rowell, C.H.F., Dorey, A.E.: The number and size of axons in the thoracic connectives of the desert locust, Schistocerca gregaria Forsk. Z. Zellforsch. 83, 288–294 (1967)Google Scholar
  45. Scharrer, B.: Neurohumors and neurohormones: definitions and terminology. J. Neuro.-Visceral Rel., Suppl. 9, 1–20 (1969)Google Scholar
  46. Strausfeld, N.J.: Atlas of an insect brain. Berlin-Heidelberg-New York: Springer 1976Google Scholar
  47. Strausfeld, N.J., Obermayer, M.: Resolution of intraneuronal and transsynaptic migration of cobalt in the insect visual and central nervous systems. J. comp. Physiol. 110, 1–12 (1976)Google Scholar
  48. Strausfeld, N.J. et al.: In pressGoogle Scholar
  49. Strong, L.: On the occurrence of neuroglandular axones within the sympathetic nervous system of the locust, Locusta migratoria migratorioides. J. roy. micr. Soc. 86, 141–149 (1966)Google Scholar
  50. Suzuki, H.: Antennal movements induced by odour and central projection of the antennal neurones in the honey-bee. J. Insect Physiol. 21, 831–847 (1975)Google Scholar
  51. Tyrer, N.M., Altman, J.S.: Motor and sensory flight neurones in a locust demonstrated using cobalt chloride. J. comp. Neurol. 157, 117–138 (1974)Google Scholar
  52. Tyrer, N.M., Bell, E.M.: The intensification of profiles of cobalt-injected neurones in sectional material. Brain Res. 73, 151–155 (1974)Google Scholar
  53. Weiss, M.J.: Neuronal connections and the function of the corpora pedunculata in the brain of the American cockroach, Periplaneta americana (L.). J. Morph. 142, 21–69 (1974)Google Scholar
  54. Willey, R.B.: The morphology of the stomodeal nervous system in Periplaneta americana (L.) and other Blattaria. J. Morph. 108, 219–261 (1961)Google Scholar
  55. Zawarzin, A.A.: Quelques données sur la structure du système nerveuse intestinal des insectes. Rev. Zool. Rüsse 1, 161–180 (1916)Google Scholar
  56. Zawarzin, A.A.: Der Parallelismus der Strukturen als ein Grundprinzip der Morphologie. Z. wiss. Zool. 124, 118–218 (1925)Google Scholar
  57. Zawarzin, A.A.: Über die morphologische Bedeutung des autonomen Nervensystems. Arch. biol. nauk 45, 17–35 (1937) [Russian]Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Elisabeth Aubele
    • 1
  • Nikolai Klemm
    • 1
  1. 1.Zoologisches Institut III, Universität WürzburgFederal Republic of Germany

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