Cell and Tissue Research

, Volume 250, Issue 3, pp 681–687 | Cite as

Postnatal differentiation of the gametogenic and endocrine functions of the testis in the tree-shrew (Tupaia belangeri)

  • P. M. Collins
  • J. Pudney
  • W. N. Tsang


Testicular development was studied in Tupaia belangeri (tree-shrew) from birth to sexual maturity. At birth the seminiferous cords contained peripheral supporting cells and centrally located gonocytes. Large foetal Leydig cells were prominent in the interstitium. The mitotic index of the gonocytes was low at birth and rose to peak levels at Day 20, following the regression of the foetal generation of Leydig cells, and during the nadir in circulating testosterone concentrations. Mitotic activity returned to low levels at Day 30 in association with the reappearance of differentiated Leydig cells and the first signs of increased androgenesis. The negative temporal relationship between mitogenesis and androgenic function suggests that the proliferation of the gonocytes does not require, and may be inhibited by, high titres of androgens. Post-mitotic development of the gonocytes occurred during a period of rising testosterone levels, and the first appearance of spermatogonia coincided with peak testosterone levels. This indicates that androgens may be specifically involved in the initiation of spermatogenesis. Spermatogenesis progressed to completion during a phase of declining testosterone levels. The precise temporal correlations established during post-natal development suggest that the tree-shrew is a suitable animal model for studies on the endocrine control of the initiation of spermatogenesis in primates.

Key words

Testis Spermatogenesis Androgens Puberty Tree-shrew (Tupaia belangeri


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  1. Abbott DH, Hearn JP (1978) Physical, hormonal and behavioural aspects of sexual development in the marmoset monkey, Callithrix jacchus. J Reprod Fertil 53:155–166Google Scholar
  2. Beaumont HM, Mandl AM (1963) A quantitative study of primordial germ cells in the male rat. J Embryol Exp Morph 11:715–740Google Scholar
  3. Clermont Y, Harvey SC (1967) Effects of hormones on spermatogenesis in the rat. Ciba Fdn Colloq Endocr 16:173–196Google Scholar
  4. Clermont Y, Perey B (1957) Quantitative study of the cell population of the seminiferous tubules in immature rats. Am J Anat 100:241–267Google Scholar
  5. Collins PM, Tsang WN (1987) Growth and reproductive development in the male tree-shrew (Tupaia belangeri) from birth to sexual maturity. Biol Repro (in press)Google Scholar
  6. Collins PM, Tsang WN, Metzger JM (1984) Influence of stress on adrenocortical function in the male tree shrew (Tupaia belangeri). Gen Comp Endocrinol 55:450–457Google Scholar
  7. Copeland KC, Kuehl TJ, Reyes P, Castracane VC (1981) The baboon as a model for puberty: growth, testis size, plasma testosterone, and somatomedin-C. Pediatr Res 15:1547AGoogle Scholar
  8. Courot M, Reviers MH, Ortavant R (1970) Spermatogenesis. In: Johnson AD, Gomes WR, Vandemark NL (eds) The Testis, vol. 1. Development, Anatomy, and Physiology. Academic Press, New York, pp 339–432Google Scholar
  9. Franchi LL, Mandl AM (1964) The ultrastructure of germ cells in foetal and neonatal male rats. J Embryol Exp Morph 12:289–308Google Scholar
  10. Gondos B, Byskov AG (1981) Germ cell kinetics in the neonatal rabbit testis. Cell Tissue Res 215:143–151Google Scholar
  11. Gondos B, Renston RH, Conner LA (1973) Ultrastructure of germ cells and Sertoli cells in the postnatal rabbit testis. Am J Anat 136:427–440Google Scholar
  12. Huckins C, Clermont Y (1968) Evolution of gonocytes in the rat testis during late embryonic and early postnatal life. Arch Anat Histol Embryol 51:343–354Google Scholar
  13. Lee VMK, Burger HG (1983) Pituitary testicular axis during pubertal development. Monogr Endocrinol 25:44–70Google Scholar
  14. Lostroh AJ (1969) Regulation by FSH and ICSH(LH) of reproductive function in the immature male rat. Endocrinology 85:438–445Google Scholar
  15. Martin DE, Swenson RB, Collins DC (1977) Correlation of serum testosterone levels with age in male chimpanzees. Steroids 29:471–481Google Scholar
  16. Noumura T, Weisz J, Lloyd CW (1966) In vitro conversion of 7-3H-progesterone to androgens by the rat testis during the second half of fetal life. Endocrinology 78:245–253Google Scholar
  17. Resko JA, Feder HH, Goy RW (1968) Androgen concentrations in plasma and testis of developing rats. J Endocr 40:485–491Google Scholar
  18. Roosen-Runge EC, Leik J (1968) Gonocyte degeneration in the postnatal male rat. Am J Anat 122:275–300Google Scholar
  19. Sapsford CS (1962) Changes in the cells of the sex cords and seminiferous tubules during the development of the testis of the rat and mouse. Aust J Zool 10:178–192Google Scholar
  20. Steinberger E, Ficher M (1968) Conversion of progesterone to testosterone by testicular tissue at different stages of maturation. Steroids 11:351–368Google Scholar
  21. Steinberger E, Steinberger A (1974) Hormonal control of testicular function in mammals. In: Geiger SR (ed), Handbook of Physiology, Endocrinology IV. Waverly Press, Inc., Baltimore, pp 325–345Google Scholar
  22. Steiner RA, Bremner WJ (1981) Endocrine correlates of sexual development in the male monkey. Macaca fascicularis. Endocrinology 109:914–919Google Scholar
  23. Tsang WN, Collins PM (1985) Techniques for hand-rearing treeshrews (Tupaia belangeri) from birth. Zool Biol 4:23–31Google Scholar
  24. Vilar O (1970) Histology of the human testis from neonatal period to adolescence. In: Rosenberg E, Paulsen A (eds), The Human Testis Plenum Press, New York, pp 95–111Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • P. M. Collins
    • 1
  • J. Pudney
    • 2
  • W. N. Tsang
    • 1
  1. 1.Department of Biological SciencesUniversity of CaliforniaSanta Barbara
  2. 2.Biological Science CenterBoston UniversityBostonUSA

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